Analysis of fermentation processes using flow microfluorometry: Amylase and protease activities inBacillis subtilis batch cultures

Maytansine, a novel ansa macrolide (1), has potent anti-tumor and antimitotic activity (2, 3). It blocks cell cycle traverse in mitosis with resultant accumulation of metaphase cells (4). Inhibition of brain tubulin polymerization in vitro by maytansine has also been reported (3). The C-mitotic effect of this drug is similar to that of the well known Vinca- alkaloids, vinblastine and vincristine. This study was carried out to examine the effects of maytansine on the cell cycle traverse and the fine struc- I ture of human lymphoblasts.Log-phase cultures of CCRF-CEM human lymphoblasts were exposed to maytansine concentrations from 10-6 M to 10-10 M for 18 hrs. Aliquots of cells were removed for cell cycle analysis by flow microfluorometry (FMF) (5) and also processed for transmission electron microscopy (TEM). FMF analysis of cells treated with 10-8 M maytansine showed a reduction in the number of G1 cells and a corresponding build-up of cells with G2/M DNA content.

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Mathematical modeling and parameters estimation of anaerobic fermentation processes

1999 European Control Conference (ECC) ◽

10.23919/ecc.1999.7099388 ◽

1999 ◽

Author(s):

Ivan Simeonov

Keyword(s):

Mathematical Modeling ◽

Anaerobic Fermentation ◽

Parameters Estimation ◽

Fermentation Processes

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0471 The effect of binding feed emzymes to spores of Bacillius subtlis and Bacillius coagulans on in vitro NDF digestibility in ruminal batch cultures

Journal of Animal Science ◽

10.2527/jam2016-0471 ◽

2016 ◽

Vol 94 (suppl_5) ◽

pp. 225-225

Author(s):

C. L. Rosser ◽

L. Jin ◽

K. A. Beauchemin ◽

M. Oba ◽

S. M. Cutting ◽

...

Keyword(s):

Batch Cultures

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The role of a metabolic intermediate in the biodegradation of inhibitory substrates

Water Science & Technology ◽

10.2166/wst.1997.0352 ◽

1997 ◽

Vol 36 (10) ◽

pp. 27-36 ◽

Cited By ~ 1

Author(s):

P. Mungkarndee ◽

S. M. Rao Bhamidimarri ◽

A. J. Mawson ◽

R. Chong

Keyword(s):

The Other ◽

Metabolic Product ◽

Dichlorophenoxyacetic Acid ◽

Mutual Inhibition ◽

Metabolic Intermediate ◽

Batch Cultures ◽

Ortho Cresol ◽

2,4 Dichlorophenoxyacetic Acid

Biodegradation of the mixed inhibitory substrates, 2,4-dichlorophenoxyacetic acid (2,4-D) and para-chloro-ortho-cresol (PCOC) was studied in aerobic batch cultures. Each substrate added beyond certain concentrations inhibited the degradation of the other. This mutual inhibition was found to be enhanced by 2,4-dichlorophenol (2,4-DCP) which is an intermediate metabolic product of 2,4-D. When 2,4-DCP accumulated to approximatelY 40 mg/l degradation of all compounds in the mixed 2,4-D and PCOC substrate system was completely inhibited. The degradation of 2,4-D and PCOC individually was also found to be inhibited by elevated concentrations of 2,4-DCP added externally, while PCOC inhibited the utilization of the intermediate.

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Activated carbon as a better habitat for water and wastewater treatment microorganisms

Water Science & Technology ◽

10.2166/wst.2000.0260 ◽

2000 ◽

Vol 42 (12) ◽

pp. 149-154 ◽

Cited By ~ 5

Author(s):

M. Okada ◽

H. Morinaga ◽

W. Nishijima

Keyword(s):

Batch Culture ◽

Bacterial Growth ◽

Membrane Filter ◽

Synthetic Medium ◽

Dialysis Tubing ◽

Water And Wastewater Treatment ◽

Batch Cultures ◽

K 12 ◽

Bacterial Yield ◽

Adsorption Desorption

Effects of PAC on bacterial activity were evaluated by sequencing batch cultures (20 hours each) of E.coli K-12 on synthetic medium containing glucose as a sole carbon source. Four suspended sequencing batch culture systems were operated; CP: cultures supplemented with PAC, CR: cultures with removal of metabolites by PAC at the end of each batch culture, CD: cultures supplemented with PAC in dialysis tubing to separate from E.coli, and CC: cultures without PAC (control). The supernatant of each batch culture was filtered through a membrane filter (0.2 μm) and was mixed with the same volume of fresh medium to be used as the medium for the next batch culture. The sequencing batch cultures were repeated three times for all the systems. The bacterial growth in CC was inhibited with the increase in the number of batch cultures. Although a significant amount of metabolites was accumulated in the 3rd batch culture of CC, little accumulation was noted in the 3rd batch culture of CP. No growth inhibition was noted in CP for all the batch cultures. The little differences in the bacterial yield and metabolite accumulation between CR and CD suggested that adsorption/desorption of metabolites with PAC did not play a major role in bacterial growth. PAC addition may partly stimulate the growth by the removal of growth inhibiting metabolites. However, the fact that CP showed higher yield than CR and CD indicated that the contact between bacteria and PAC plays a significant role in the growth of bacteria.

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The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19900854 ◽

1990 ◽

Vol 55 (3) ◽

pp. 854-866 ◽

Cited By ~ 5

Author(s):

Rodríguez V. Bravo ◽

Rubio F. Camacho ◽

Villasclaras S. Sánchez ◽

Vico M. Castro

Keyword(s):

Cell Growth ◽

Ethanol Production ◽

Yeast Extract ◽

Culture Medium ◽

Ethanolic Fermentation ◽

Pachysolen Tannophilus ◽

Significant Component ◽

Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

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