Enhanced ammonia detoxification to urea in hepatocytes transduced with human aquaporin‐8 gene

2021 ◽  
Author(s):  
Alejo M. Capiglioni ◽  
Gabriela L. Muller ◽  
Julieta Marrone ◽  
Maria de Lujan Alvarez ◽  
Raúl A. Marinelli
Keyword(s):  
Ammonia Detoxification

scholarly journals Ammonia detoxification via ureagenesis in rat hepatocytes involves mitochondrial aquaporin-8 channels

Hepatology ◽  
2013 ◽  
Vol 57 (5) ◽  
pp. 2061-2071 ◽  
Author(s):  
Leandro R. Soria ◽  
Julieta Marrone ◽  
Giuseppe Calamita ◽  
Raúl A. Marinelli
Keyword(s):  
Rat Hepatocytes ◽  
Ammonia Detoxification

scholarly journals The effect of rewarming media composition on the ammonia detoxification ability of cold preserved rat hepatocytes

2002 ◽  
Vol 1 (3) ◽  
pp. 140-146 ◽  
Author(s):  
Sebastián Dante Calligaris ◽  
Edgardo Elvio Guibert ◽  
Joaquín Valentín Rodríguez
Keyword(s):  
Rat Hepatocytes ◽  
Media Composition ◽  
Ammonia Detoxification

Incorporation of 15N-Ammonia into Serotonin in Cotyledons of Maturing Walnuts

1983 ◽  
Vol 38 (11-12) ◽  
pp. 1057-1058 ◽  
Author(s):  
Wolfgang Große ◽  
Francesc Artigas
Keyword(s):  
Juglans Regia ◽  
Preparative Hplc ◽  
Ammonia Detoxification ◽  
N Incorporation

Cotyledons isolated from maturing seeds of Juglans regia incorporate supplied [15N]-ammonia into serotonin (5-HT). A 15N incorporation of about 9% into the indolic nitrogen of 5-HT is detectable by gas chromatographymass spectrometry following isolation of 5-HT by preparative HPLC. This 15N incorporation supports the hypothesis that biosynthesis and accum ulation of 5-HT play a role in ammonia detoxification in maturing walnut seeds

Comprehensive characterization of ureagenesis in the spf ash mouse, a model of human ornithine transcarbamylase deficiency, reveals age‐dependency of ammonia detoxification

2019 ◽  
Vol 42 (6) ◽  
pp. 1064-1076 ◽  
Author(s):  
Gabriella Allegri ◽  
Sereina Deplazes ◽  
Nicole Rimann ◽  
Benjamin Causton ◽  
Tanja Scherer ◽  
...  
Keyword(s):  
Ornithine Transcarbamylase ◽  
Ornithine Transcarbamylase Deficiency ◽  
Age Dependency ◽  
Ammonia Detoxification ◽  
Comprehensive Characterization

scholarly journals Hepatic glutamine synthetase augmentation enhances ammonia detoxification

2019 ◽  
Vol 42 (6) ◽  
pp. 1128-1135 ◽  
Author(s):  
Leandro R. Soria ◽  
Matthew Nitzahn ◽  
Angela De Angelis ◽  
Suhail Khoja ◽  
Sergio Attanasio ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Ammonia Detoxification

Encapsulation of glutamine synthetase in mouse erythrocytes: a new procedure for ammonia detoxification

2008 ◽  
Vol 86 (6) ◽  
pp. 469-476 ◽  
Author(s):  
Elena A. Kosenko ◽  
Natalia I. Venediktova ◽  
Andrey A. Kudryavtsev ◽  
Fazoil I. Ataullakhanov ◽  
Yury G. Kaminsky ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Glutamine Synthetase ◽  
Synthetase Activity ◽  
Glutamine Synthetase Activity ◽  
Cell Recovery ◽  
Ammonia Detoxification ◽  
Neurological Alterations

There are a number of pathological situations in which ammonia levels increase leading to hyperammonemia, which may cause neurological alterations and can lead to coma and death. Currently, there are no efficient treatments allowing rapid and sustained decrease of ammonia levels in these situations. A way to increase ammonia detoxification would be to increase its incorporation in glutamine by glutamine synthetase. The aim of this work was to develop a procedure to encapsulate glutamine synthetase in mouse erythrocytes and to assess whether administration of these erythrocytes containing glutamine synthetase (GS) reduce ammonia levels in hyperammonemic mice. The procedure developed allowed the encapsulation of 3 ± 0.25 IU of GS / mL of erythrocytes with a 70% cell recovery. Most metabolites, including ATP, remained unaltered in glutamine synthetase-loaded erythrocytes (named ammocytes by us) compared with native erythrocytes. The glutamine synthetase-loaded ammocytes injected in mice survived and retained essentially all of their glutamine synthetase activity for at least 48 h in vivo. Injection of these ammocytes into hyperammonemic mice reduced ammonia levels in the blood by about 50%. The results reported indicate that ammocytes are able to keep their integrity, normal energy metabolism, the inserted glutamine synthetase activity, and can be useful to reduce ammonia levels in hyperammonemic situations.

scholarly journals Glutamine Synthetase in Muscle Is Required for Glutamine Production during Fasting and Extrahepatic Ammonia Detoxification

2010 ◽  
Vol 285 (13) ◽  
pp. 9516-9524 ◽  
Author(s):  
Youji He ◽  
Theodorus B. M. Hakvoort ◽  
S. Eleonore Köhler ◽  
Jacqueline L. M. Vermeulen ◽  
D. Rudi de Waart ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Ammonia Detoxification ◽  
Glutamine Production

Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells

2002 ◽  
Vol 283 (6) ◽  
pp. G1264-G1275 ◽  
Author(s):  
Eiji Nakamura ◽  
Susan J. Hagen
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Ammonium Chloride ◽  
Gastric Epithelial Cells ◽  
Cytotoxic Factor ◽  
Ammonia Detoxification ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
Detoxification Pathways

Ammonia is a cytotoxic factor produced during Helicobacter pylori infection that may reduce the survival of surface epithelial cells. Here we examine whether ammonia kills cells and whether l-glutamine (l-Gln) protects against cell death by stimulating ammonia detoxification pathways. Cell viability and vacuolation were quantified in rat gastric epithelial (RGM1) cells incubated with ammonium chloride at pH 7.4 in the presence or absence of l-Gln. Incubation of RGM1 cells with ammonium chloride caused a dose-dependent increase in cell death and vacuolation, which were both inhibited byl-Gln. We show that RGM1 cells metabolize ammonia to urea via arginase, a process that is stimulated by l-Gln and results in reduced ammonia cytotoxicity. l-Gln also inhibits the uptake and facilitates the extrusion of ammonia from cells. Blockade of glutamine synthetase did not reduce the survival of RGM1 cells, demonstrating that the conversion ofl-glutamate and ammonia to l-Gln is not involved in ammonia detoxification. Thus our data support a role forl-Gln and arginase in protection against ammonia-induced cell death in gastric epithelial cells.

Stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells improves its ammonia detoxification

2012 ◽  
Vol 113 (2) ◽  
pp. 518-527 ◽  
Author(s):  
Nanhong Tang ◽  
Yan Wang ◽  
Xiaoqian Wang ◽  
Liangyi Zhou ◽  
Feiyuan Zhang ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Ornithine Transcarbamylase ◽  
Ammonia Detoxification ◽  
Arginase I

Medium-chain triglycerides supplement therapy with a low-carbohydrate formula can supply energy and enhance ammonia detoxification in the hepatocytes of patients with adult–onset type II citrullinemia

2018 ◽  
Vol 41 (5) ◽  
pp. 777-784 ◽  
Author(s):  
Kiyoshi Hayasaka ◽  
Chikahiko Numakura ◽  
Mitsunori Yamakawa ◽  
Tetsuo Mitsui ◽  
Hisayoshi Watanabe ◽  
...  
Keyword(s):  
Type Ii ◽  
Adult Onset ◽  
Medium Chain Triglycerides ◽  
Medium Chain ◽  
Onset Type ◽  
Ammonia Detoxification ◽  
Low Carbohydrate ◽  
Supplement Therapy
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