Tangential Flow Filtration Facilitated Fractionation and PEGylation of Low and High Molecular Weight Polymerized Hemoglobins and Their Biophysical Properties

2021 ◽  
Author(s):  
Xiangming Gu ◽  
Chintan Savla ◽  
Andre F. Palmer
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Biophysical Properties ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Separation of natural organic colloids with a PALL tangential flow filtration system

2009 ◽  
Vol 9 (5) ◽  
pp. 583-590 ◽  
Author(s):  
Berit Schwalger ◽  
Alejandro Spitzy
Keyword(s):  
Molecular Weight ◽  
Chemical Properties ◽  
Low Carbon ◽  
Size Fractionation ◽  
Membrane Cleaning ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Filtration System ◽  
Physico Chemical ◽  
Flow Filtration

The applicability of a PALL tangential flow filtration (TFF) system for size fractionation of natural dissolved organic matter was investigated. The performance of polyethersulfone membranes with nominal molecular weight cut-off of 1 kDa, 5 kDa and 50 kDa was examined for isolation of low and high molecular weight compounds in fresh and estuarine waters with diverse physico-chemical properties. Detailed protocols for operating the TFF- system and for membrane cleaning are proposed. The ultrafiltration membranes can be efficiently cleaned to provide low carbon blanks (<0.09 mg/l). Standard colloid tests confirmed that the higher molecular weight compounds were isolated in the retentate and the lower molecular weight compounds remain in the permeate. Mass balance of fractionated natural samples showed good recoveries for dissolved organic carbon (DOC) (99±13% (1 kDa); 103±20% (5 kDa); 94±14% (50 kDa) (n=9). Moreover, high ionic strength or high DOC content did not enhance either fouling or contamination of the membrane. These findings demonstrate that the PALL TFF system is reliable for natural organic colloids fractionation in aquatic systems across both salinity and DOC gradients.

Concentration of live pico- and nanoplankton by means of tangential flow filtration

1989 ◽  
Vol 11 (6) ◽  
pp. 1213-1221 ◽  
Author(s):  
Klaus-Günther Barthel ◽  
Gerald Schneider ◽  
Rolf Gradinger ◽  
Jürgen Lenz
Keyword(s):  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

High performance tangential flow filtration

1997 ◽  
Vol 56 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Robert van Reis ◽  
Shishir Gadam ◽  
Leah N. Frautschy ◽  
Scott Orlando ◽  
Elizabeth M. Goodrich ◽  
...  
Keyword(s):  
High Performance ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Author response for "Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N‐1 perfusion seed culture"

2020 ◽  
Author(s):  
Daniel J Karst ◽  
Kevin Ramer ◽  
Erik H Hughes ◽  
Canping Jiang ◽  
Pieter J Jacobs ◽  
...  
Keyword(s):  
Author Response ◽  
Transmembrane Pressure ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Seed Culture ◽  
Flow Filtration

Tangential flow filtration of haptoglobin

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Ivan S. Pires ◽  
Andre F. Palmer
Keyword(s):  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Removal of RNA impurities by tangential flow filtration in an RNase-free plasmid DNA purification process

2003 ◽  
Vol 316 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Alex Eon-Duval ◽  
Robert H MacDuff ◽  
Carol A Fisher ◽  
Mark J Harris ◽  
Chris Brook
Keyword(s):  
Plasmid Dna ◽  
Dna Purification ◽  
Purification Process ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration
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