Cole‐Cole modeling of real‐time capacitance data for estimation of cell physiological properties in recombinant Escherichia coli cultivation

2021 ◽  
Author(s):  
Nivedhitha Swaminathan ◽  
Priyanka Priyanka ◽  
Anurag S. Rathore ◽  
Senthilkumar Sivaparakasam ◽  
Senthilmurugan Subbiah
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Recombinant Escherichia Coli ◽  
Physiological Properties

Real-Time Detection on the Expression of Soluble Protein and Inclusion Body in the Recombinant Escherichia coli with Laser Tweezers Raman Spectroscopy

2014 ◽  
Vol 41 (12) ◽  
pp. 1215003
Author(s):  
黄庶识 Huang Shushi ◽  
卢明倩 Lu Mingqian ◽  
李冰 Li Bing ◽  
周冰 Zhou Bing ◽  
陈丽梅 Chen Limei
Keyword(s):  
Escherichia Coli ◽  
Raman Spectroscopy ◽  
Real Time ◽  
Inclusion Body ◽  
Soluble Protein ◽  
Recombinant Escherichia Coli ◽  
Laser Tweezers ◽  
Real Time Detection

scholarly journals Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing Escherichia coli (STEC)

2020 ◽  
Vol 8 (11) ◽  
pp. 1801
Author(s):  
Michael Bording-Jorgensen ◽  
Brendon D. Parsons ◽  
Gillian A.M. Tarr ◽  
Binal Shah-Gandhi ◽  
Colin Lloyd ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Gene Detection ◽  
Culture Positivity ◽  
Hands On ◽  
Culture Independent ◽  
Chromogenic Agar ◽  
Stool Samples

Shiga toxin-producing Escherichia coli (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both stx genes’ amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which stx gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3–5 days until a sample is negative by culture.

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