High-performance liquid chromatography method development and validation for simultaneous determination of five model compounds, antipyrine, metoprolol, ketoprofen, furosemide and phenol red, as a tool for the standardization of ratin situ intestinal permeability studies using timed wavelength detection

An isocratic Reversed-Phase High Performance Liquid Chromatography method has been developed for rapid and simultaneous separation and estimation of two antibiotics, namely, nitazoxanide and ofloxacin, in human plasma. Separation was carried out on Altima C8 (150 x 4.6 mm, 5µ) column using a mobile phase of 0.1% ortho phosphoric acid: acetonitrile (50:50, V/V) at 260 nm. The retention time of nitazoxanide and ofloxacin was noted to be 4.850 and 7.949 min, respectively. The average % recovery for nitazoxanide and ofloxacin were 98.012 % and 94.176 %, respectively and reproducibility was found to be satisfactory. The linearity was investigated in the concentration range of 0.02-2 µg/ml (r2=0.9996) for nitazoxanide and 0.008-0.8 µg/ml (r2=0.9998) for ofloxacin. The lower limits of quantification were 0.0196 µg/ml and 0.0079 µg/ml for nitazoxanide and ofloxacin, respectively, which reach the level of both drugs possibly found in human plasma. The proposed method can be applied for etermination of nitazoxanide and ofloxacin from dosage forms during pharmacokinetic study.

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Rapid resolution liquid chromatography method development and validation for simultaneous determination of homocysteine, vitamins B6, B9, and B12in human serum

Indian Journal of Pharmacology ◽

10.4103/0253-7613.108303 ◽

2013 ◽

Vol 45 (2) ◽

pp. 159 ◽

Cited By ~ 1

Author(s):

SiewHua Gan ◽

MunvarMiya Shaik

Keyword(s):

Liquid Chromatography ◽

Human Serum ◽

Simultaneous Determination ◽

Method Development ◽

Chromatography Method ◽

Rapid Resolution ◽

Method Development And Validation ◽

Liquid Chromatography Method ◽

Development And Validation

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Bioanalytical Method Development and Validation of Naproxen: Application to Bioequivalence Studies

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v8i2.8502 ◽

2017 ◽

Vol 8 (2) ◽

Author(s):

Senthil Rajan Dharmalingam ◽

Srinivasan Ramamurthy ◽

Sai Siddhardh ◽

M. D. Basheerudhin

Keyword(s):

Human Plasma ◽

Mobile Phase ◽

High Performance ◽

Method Development ◽

Diclofenac Sodium ◽

Pharmacokinetic Studies ◽

Chromatography Method ◽

Method Development And Validation ◽

Liquid Chromatography Method ◽

Development And Validation

A new selective and sensitive high-performance liquid chromatography method was developed for the quantification of Naproxen in human plasma using diclofenac sodium asinternal standard (IS). Chromatographic separation was achieved on aPhenomenex GEMINI C18 (150 x 4.6 mm, 5 mm) column. The mobile phase consists of a mixture of Acetonitrile: 0.5% Triethylamine buffer (50:50; v/v) and the pH of the mobile phase was adjusted to 3.5 by 85 % orthophosphoric acid. Flow rate of mobile phase was 1 mL/min.Detection was performed at 230nm. The calibration curve was linear over the concentration range from 10 to 120µg/mL. The detection (LOD) and quantification (LOQ) limits were 10 ng/mL and 25 ng/Ml respectively. The method was validated for accuracy, precision, specificity, robustness, and detection and quantification limits, in accordance with ICH guidelines.The developed method for the determination of Naproxen from human plasma has been found accurate, precise, selective, and suitable for the bioequivalence and pharmacokinetic studies.

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