Assessments of growth conditions on the production of cyanophycin by recombinant Escherichia coli strains expressing cyanophycin synthetase gene

2012 ◽  
Vol 28 (2) ◽  
pp. 358-363 ◽  
Author(s):  
Wen-Chi Tseng ◽  
Tsuei-Yun Fang ◽  
Chiung-Yu Cho ◽  
Po-Shao Chen ◽  
Ching-Shiang Tsai
Keyword(s):  
Escherichia Coli ◽  
Growth Conditions ◽  
Recombinant Escherichia Coli ◽  
Cyanophycin Synthetase

Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion

2000 ◽  
Vol 45 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Man Bock Gu ◽  
Jiho Min ◽  
Robert A LaRossa
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli

Development of a fermentation strategy to enhance the catalytic efficiency of recombinant Escherichia coli for l-2-aminobutyric acid production

3 Biotech ◽  
2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Jian-Miao Xu ◽  
Ming Wang ◽  
Yi-Hua Jin ◽  
Zhi-Qiang Liu ◽  
Yu-Guo Zheng
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Catalytic Efficiency ◽  
Recombinant Escherichia Coli ◽  
Aminobutyric Acid ◽  
Fermentation Strategy

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

d-Mannitol formation from d-glucose in a whole-cell biotransformation with recombinant Escherichia coli

2005 ◽  
Vol 69 (4) ◽  
pp. 397-403 ◽  
Author(s):  
Björn Kaup ◽  
Stephanie Bringer-Meyer ◽  
Hermann Sahm
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Biotransformation

Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol

2021 ◽  
Author(s):  
Mesfin Angaw Tesfay ◽  
Xin Wen ◽  
Yujie Liu ◽  
Huibin Lin ◽  
Linxu Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli

scholarly journals Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli

2021 ◽  
Author(s):  
Wenjun Li ◽  
Chengbo Ma ◽  
Baosheng Ge ◽  
Fan Zang ◽  
Song Qin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli
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