Expression of exo-inulinase gene fromAspergillus niger12 inE. colistrain Rosetta-gami B (DE3) and its characterization

2016 ◽  
Vol 32 (3) ◽  
pp. 629-637 ◽  
Author(s):  
Shreyas S. Yedahalli ◽  
Lars Rehmann ◽  
Amarjeet Bassi
Keyword(s):  

2014 ◽  
Vol 49 (11) ◽  
pp. 1867-1874 ◽  
Author(s):  
Hai-Xiang Zhou ◽  
Fang-Hua Xin ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi


2010 ◽  
Vol 12 (6) ◽  
pp. 510-517 ◽  
Author(s):  
Chun-Hai Zhao ◽  
Wei Cui ◽  
Xiao-Yan Liu ◽  
Zhen-Ming Chi ◽  
Catherine Madzak


2008 ◽  
Vol 136 ◽  
pp. S298 ◽  
Author(s):  
Jing Yu ◽  
Jiaxi Jiang ◽  
Zian Fang ◽  
Yuyang Li ◽  
Jianping Liu


2014 ◽  
Vol 57 (4) ◽  
pp. 337-347 ◽  
Author(s):  
Lin-Lin Zhang ◽  
Mei-Juan Tan ◽  
Guang-Lei Liu ◽  
Zhe Chi ◽  
Guang-Yuan Wang ◽  
...  


FEBS Letters ◽  
1991 ◽  
Vol 289 (1) ◽  
pp. 64-68 ◽  
Author(s):  
Olivier Laloux ◽  
Jean-Pol Cassart ◽  
Jean Delcour ◽  
Jozef Van Beeumen ◽  
Jean Vandenhaute


2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.



Gene ◽  
2012 ◽  
Vol 495 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Shinya Kuzuwa ◽  
Ken-ji Yokoi ◽  
Mitsuru Kondo ◽  
Hisashi Kimoto ◽  
Ayanori Yamakawa ◽  
...  


2006 ◽  
Vol 101 (3) ◽  
pp. 277-279 ◽  
Author(s):  
Satoshi Moriyama ◽  
Michio Muguruma ◽  
Kazuyoshi Ohta


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