Developing Monoclonal Antibodies for Immunohistochemistry

The experiences of a laboratory which pioneered the application of monoclonal antibodies to diagnostic histochemistry is described. This was achieved in four key steps: (1) Monoclonal antibodies were successfully produced to replace the difficult-to-produce and limited polyclonal antibodies available for immunohistochemistry. (2) Monoclonal antibodies were produced to improve the immunoenzymatic detection of bound antibodies, using immunoperoxidase or alkaline phosphatase, increasing sensitivity and allowing the use of two chromogens when applied together. The availability of a reliable alkaline phosphatase-based detection allowed the detection of antigens in tissues with high endogenous peroxidase. (3) Methodologies were developed to unmask antigens not detected in routinely processed paraffin-embedded tissue. (4) Synthetic peptides were used as immunising antigens for the direct production of specific molecules of diagnostic interest. This was expanded to include recombinant proteins. Many reacted with fixed tissue and recognised homologous molecules in other species. In addition to these developments, the laboratory promoted the collaboration and training of researchers to spread the expertise of monoclonal production for diagnosis.

Implementation of Innovative Manufacturing Technologies in Foundries for Large-Volume Components

The development of new manufacturing technologies opens up new perspectives for the production of propellers (diameter < 5 m), especially since the use of the established sand casting process as a technology is only partially competitive in today’s market. Therefore, different applications of generative manufacturing methods for the implementation into the production process were investigated. One approach is the mould production using additive manufacturing processes. Investigations showed that especially for large components with high wall thicknesses available systems and processes for sand casting mould production are cost-intensive and conditionally suitable. With our development of a large-format FDM printer, however, the direct production of large-format positive moulds for, for example, yacht propellers up to 4 m in diameter is possible. Due to the comparatively low accuracy requirements for the mould, the focus is on the durability of the drive system and the rigidity of this FDM printer. Equipped with simple linear technology in portal design and cubic design of the frame structure with rigid heated print bed, the aim is to achieve maximum material extrusion via the print head. The production of plastic models not only facilitates handling during the moulding process, but also allows considerable time and cost savings to be made during the running process. A further step in our development is the direct production of the components using WAAM. A possible concept for robot-supported build-up welding for the production of new innovative propeller geometries is presented using the example of a hollow turbine blade for a tidal power plant.

Enhanced Catalytic Hydrogen Peroxide Production from Hydroxylamine Oxidation on Modified Activated Carbon Fibers: The Role of Surface Chemistry

Herein, direct production of hydrogen peroxide (H2O2) through hydroxylamine (NH2OH) oxidation by molecular oxygen was greatly enhanced over modified activated carbon fiber (ACF) catalysts. We revealed that the higher content of pyrrolic/pyridone nitrogen (N5) and carboxyl-anhydride oxygen could effectively promote the higher selectivity and yield of H2O2. By changing the volume ratio of the concentrated H2SO4 and HNO3, the content of N5 and surface oxygen containing groups on ACF were selectively tuned. The ACF catalyst with the highest N5 content and abundant carboxyl-anhydride oxygen containing groups was demonstrated to have the highest activity toward catalytic H2O2 production, enabling the selectivity of H2O2 over 99.3% and the concentration of H2O2 reaching 123 mmol/L. The crucial effects of nitrogen species were expounded by the correlation of the selectivity of H2O2 with the content of N5 from X-ray photoelectron spectroscopy (XPS). The possible reaction pathway over ACF catalysts promoted by N5 was also shown.

Heterologous expression of a polyketide synthase ACRTS2 in Aspregillus oryzae produces host selective ACR-toxins: Co-production of minor metabolites

Previously, we succeeded to produce the core structure of the host-selective ACR-toxin (1) on brown leaf spot on rough lemon when the polyketide synthase ACRTS2 gene was heterologously expressed in Aspergillus oryzae (AO). To confirm the production of 1 in AO, the detection limit and suppressing decarboxylation were improved, and these efforts led us to conclude the direct production of 1 instead of its decarboxylation product. During this examination, minor ACR-toxin-related metabolites were found. Their structure determination enabled us to propose a decarboxylation mechanism and novel branching route forming byproducts from the coupling of the dihydropyrone moiety of 1 with the acetaldehyde and kojic acid abundant in AO. The involvement of putative cyclase ACRTS3 in the chain release of linear polyketide was excluded by the co-expression analysis of ACRTS2 and ACRTS3. Taken together, we concluded the production of 1 in AO is solely responsible for ACRTS2.

Metal Sulfide Precipitation: Recent Breakthroughs and Future Outlooks

The interest in metal sulfide precipitation has recently increased given its capacity to efficiently recover several metals and metalloids from different aqueous sources, including wastewaters and hydrometallurgical solutions. This article reviews recent studies about metal sulfide precipitation, considering that the most relevant review article on the topic was published in 2010. Thus, our review emphasizes and focuses on the overall process and its main unit operations. This study follows the flow diagram definition, discussing the recent progress in the application of this process on different aqueous matrices to recover/remove diverse metals/metalloids from them, in addition to kinetic reaction and reactor types, different sulfide sources, precipitate behavior, improvements in solid–liquid separation, and future perspectives. The features included in this review are: operational conditions in terms of pH and Eh to perform a selective recovery of different metals contained in an aqueous source, the aggregation/colloidal behavior of precipitates, new materials for controlling sulfide release, and novel solid–liquid separation processes based on membrane filtration. It is therefore relevant that the direct production of nanoparticles (Nps) from this method could potentially become a future research approach with important implications on unit operations, which could possibly expand to several applications.

Influence of Veneer Density on Plywood Thickness and Some Mechanical Properties

It has been common knowledge that as the density of wood increases, the mechanical properties also improve. In turn, the density of wood depends on many factors, including the wood moisture content, location and cross-section in the trunk, the type of treatment and the parameters of technological processes. There is a great deal of research reported in the scientific literature on the effect of solid wood density on mechanical properties for different wood species as well as for structural timber. However, no research data can be found related investigation of the influence of veneer density on the properties of the birch plywood. In the present study, researching the properties of 7-ply birch plywood (thickness 9 mm), it was concluded that as the density of veneers increases, the bending properties of plywood in the direction of wood fibers (covered veneers) increases. When determining the plywood gluing quality, similar tendencies have been observed. For plywood with a lower density in all veneer plies the gluing quality (tensile-shear test) for perpendicular wood fiber veneers increases in the direction from the symmetry axis or middle veneer to the plywood outer plies, which can be explained by the fact that the outer plies become denser at the time of the hot pressing process. The results of the study will allow birch plywood manufacturers in direct production, sort veneers by density, to produce plywood with very predictable gluing quality, plywood thickness and mechanical properties in bending.

Bottomonium production and polarization in the NRQCD with $$k_T$$-factorization. III: $$\Upsilon (1S)$$ and $$\chi _b(1P)$$ mesons

The $$\Upsilon (1S)$$ Υ ( 1 S ) meson production and polarization at high energies is studied in the framework of the $$k_T$$ k T -factorization approach. Our consideration is based on the non-relativistic QCD formalism for a bound states formation and off-shell production amplitudes for hard partonic subprocesses. The direct production mechanism, feed-down contributions from radiative $$\chi _b(mP)$$ χ b ( m P ) decays and contributions from $$\Upsilon (3S)$$ Υ ( 3 S ) and $$\Upsilon (2S)$$ Υ ( 2 S ) decays are taken into account. The transverse momentum dependent (TMD) gluon densities in a proton were derived from the Ciafaloni–Catani–Fiorani–Marchesini evolution equation and the Kimber-Martin–Ryskin prescription. Treating the non-perturbative color octet transitions in terms of multipole radiation theory, we extract the corresponding non-perturbative matrix elements for $$\Upsilon (1S)$$ Υ ( 1 S ) and $$\chi _b(1P)$$ χ b ( 1 P ) mesons from a combined fit to transverse momenta distributions measured at various LHC experiments. Then we apply the extracted values to investigate the polarization parameters $$\lambda _\theta $$ λ θ , $$\lambda _\phi $$ λ ϕ and $$\lambda _{\theta \phi }$$ λ θ ϕ , which determine the $$\Upsilon (1S)$$ Υ ( 1 S ) spin density matrix. Our predictions have a reasonably good agreement with the currently available Tevatron and LHC data within the theoretical and experimental uncertainties.

A Study on Drivers of Water Consumption in China From a Complex Network Perspective

Water consumption has been one of the most important topics in the field of environment and economy. Even though the driving factors of water consumption have been well studied, it is still a daunting task to reveal the influence of the status of provinces in the entire supply chain. By combining the multi-regional input-output (MRIO) model and complex network theory, an inter-provincial virtual water transfer (V WT) network was constructed to analyze the overall structural characteristics of the network model and identify the structural roles of each province. The constructed inter-provincial V WT network exhibited the characteristics of a small-world network, that is, virtual water can be easily transferred from one province to another. Moreover, network analysis revealed that provinces with different positions in the V WT network played discrepant structural roles. Panel regression analysis was further used to quantify the impact of provincial structural roles on their water consumption. The results showed that water consumption in China largely depended on some structural role characteristics in the V WT network. Out-degree and out-strength characterizing the ability of direct exporting virtual water exerted significant positive influences, while in-closeness featuring the indirect virtual water importing rate had a significant negative effect on water usage. This indicated that adjusting the uneven provincial consumption structure, the direct production demand of downstream provinces and the indirect production activities in the supply chain would help reduce water consumption. Therefore, to come true the goal of water conservation in China, it would be necessary to improve the trade structure between direct and indirect exporters and importers in the entire supply chain.