Integration of high‐throughput analytics and cell imaging enables direct early productivity and product quality assessment during Chinese Hamster ovary cell line development for a complex multi‐subunit vaccine antigen

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Xiangming Li ◽  
Yujian Zhang ◽  
Li Jing ◽  
Zongming Fu ◽  
Ou Ma ◽  
...  
Keyword(s):  
Quality Assessment ◽  
High Throughput ◽  
Chinese Hamster Ovary ◽  
Subunit Vaccine ◽  
Cell Imaging ◽  
Chinese Hamster ◽  
Vaccine Antigen ◽  
Ovary Cell ◽  
Cell Line Development ◽  
Ovary Cell Line

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

scholarly journals Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.

1981 ◽  
Vol 1 (12) ◽  
pp. 1069-1076 ◽  
Author(s):  
R J Kaufman ◽  
R T Schimke
Keyword(s):  
Dihydrofolate Reductase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Ovary Cells ◽  
Methotrexate Resistance ◽  
Gene Copies ◽  
Methotrexate Concentration ◽  
Ovary Cell Line

During stepwise increases in the methotrexate concentration in culture medium, we selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional to the number of dihydrofolate reductase gene copies (i.e., gene amplification). We studied the dihydrofolate reductase levels in individual cells that underwent the initial steps of methotrexate resistance by using the fluorescence-activated cell sorter technique. Such cells constituted a heterogeneous population with differing dihydrofolate reductase levels, and they characteristically lost the elevated enzyme levels when they were grown in the absence of methotrexate. The progeny of individual cells with high enzyme levels behaved differently and could lose all or variable numbers of the amplified genes.

Lipid Requirements of a Recombinant Chinese Hamster Ovary Cell Line (CHO)

2006 ◽  
pp. 205-207 ◽  
Author(s):  
Jeffrey T. Mcgrew ◽  
Cheryl L. Richards ◽  
Pauline Smidt ◽  
Bradley Dell ◽  
Virginia Price
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Ovary Cell Line
Sign in / Sign up

Export Citation Format

Share Document