SET domain-containing protein 5 is required for expression of primordial germ cell specification-associated genes in murine embryonic stem cells

2017 ◽  
Vol 35 (5) ◽  
pp. 247-253 ◽  
Author(s):  
Seung Eun Yu ◽  
Min Seong Kim ◽  
Su Hyung Park ◽  
Byong Chul Yoo ◽  
Kyung Hee Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Set Domain ◽  
Cell Specification ◽  
Murine Embryonic Stem Cells ◽  
Germ Cell Specification

scholarly journals Primordial Germ Cell Specification from Embryonic Stem Cells

PLoS ONE ◽  
2008 ◽  
Vol 3 (12) ◽  
pp. e4013 ◽  
Author(s):  
Wei Wei ◽  
Tingting Qing ◽  
Xin Ye ◽  
Haisong Liu ◽  
Donghui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Cell Specification ◽  
Germ Cell Specification

scholarly journals Resf1 supports embryonic stem cell self-renewal and effective germline entry

2021 ◽  
Author(s):  
Matus Vojtek ◽  
Ian Chambers
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Germ Line ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Cell Specification ◽  
Self Renewal ◽  
Downstream Target ◽  
Germ Cell Specification

Retroelement silencing factor 1 (Resf1) interacts with the key regulators of mouse embryonic stem cells (ESCs) Oct4 and Nanog, and its absence results in sterility of mice. However, the function of Resf1 in ESCs and germ line specification is poorly understood. In this study, we used Resf1 knockout cell lines to determine the requirements of RESF1 for ESCs self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells (PGCLCs). We found that deletion of Resf1 in ESCs cultured in serum and LIF reduces self-renewal potential whereas episomal expression of RESF1 has a modest positive effect on ESC self-renewal. In addition, RESF1 is not required for the capacity of NANOG and its downstream target ESRRB to drive self-renewal in the absence of LIF. However, Resf1 deletion reduces efficiency of PGCLC differentiation in vitro. These results identify Resf1 as a novel player in the regulation of pluripotent stem cells and germ cell specification.

210 DEFINING THE MINIMAL PROMOTER REGION OF BOVINE VASA HOMOLOGUE (Bvh) BY IN SILICO ANALYSIS

2013 ◽  
Vol 25 (1) ◽  
pp. 253
Author(s):  
L. F. Malaver-Ortega ◽  
H. Sumer ◽  
P. J. Verma
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Promoter Region ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Regulatory Elements ◽  
Upstream Region ◽  
Functional Level ◽  
Cell Specification ◽  
Germ Cell Specification

The DEAD box polypeptide 4, DDX4 or VASA, is a highly conserved gene that encodes a putative RNA helicase with the motif DEAD (Asp-Glu-Ala-Asp). Although little is known about its role in germ cell genesis, VASA is one of the earliest, specialised markers of primordial germ cell (PGC) specification. Furthermore, this process of specification has been recapitulated to some degree in vitro using embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) in mice and humans, using VASA expression as one of the criteria for differentiation and sorting of the differentiated cells. In order to establish a system for isolation and tracking of bovine iPSC undergoing germ cell specification, we analysed all regulatory elements in the 5-kb upstream region [RC 5083564–5088564 Bos taurus (Hereford) chromosome 20 genomic scaffold: NW_003104511.1] of the bovine VASA homologue (Bvh) locus, which is thought to be the putative promoter region of Bvh, and in the in vivo validated promoter regions, for the corresponding homologous genes in human and mouse. We performed the analysis using 2 different approaches: at the sequence level, by orthologous promoter alignment of transcription factor (TF) binding sites (TFBS) using DiAling®, and at the functional level, by functional unit analysis (complex model) using Frameworker® (Genomatix, Munich, Germany). The initial DiAling® analysis did not produce similarities between the 3 analysed species. In contrast, using the complex analysis of functional units, we identified 85 single elements common to all 3, and 795, 482, and 129 models composed of 2, 3, and 4 elements, respectively. The number of models was reduced to 3 [M1, M2, and M3 (P = 4.8 × 10–11)] by increasing the number of TF (each model composed of 6 different elements). As a result, members of SOX/SRY-sex/testis determining and related HMG box factor family related with germ cell specification, pluripotent-related factors such as members of the octamer binding protein family, and TFs common to numerous vertebrate genes such as homeobox transcription factors were identified (Table 1). Based on these results, we determined a region of approximate 0.6 kb upstream of the Bvh gene, which encloses a core of TFBS conserved at the functional level between species. We propose that this sequence is the best candidate for driving the expression of reporter genes under Bvh promoter control. Table 1.Six element models

scholarly journals Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry

2021 ◽  
Vol 4 (12) ◽  
pp. e202101190
Author(s):  
Matúš Vojtek ◽  
Ian Chambers
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Cell Specification ◽  
Self Renewal ◽  
Downstream Target ◽  
Germ Cell Specification ◽  
Positive Effect

Retroelement silencing factor 1 (RESF1) interacts with the key regulators of mouse embryonic stem cells (ESCs) OCT4 and NANOG, and its absence results in sterility of mice. However, the function of RESF1 in ESCs and germline specification is poorly understood. In this study, we used Resf1 knockout cell lines to determine the requirements of RESF1 for ESC self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells (PGCLCs). We found that deletion of Resf1 in ESCs cultured in serum and LIF reduces self-renewal potential, whereas episomal expression of RESF1 has a modest positive effect on ESC self-renewal. In addition, RESF1 is not required for the capacity of NANOG and its downstream target ESRRB to drive self-renewal in the absence of LIF. However, Resf1 deletion reduces the efficiency of PGCLC differentiation in vitro. These results identify Resf1 as a novel player in the regulation of pluripotent stem cells and germ cell specification.

scholarly journals Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells

2015 ◽  
Vol 16 (5) ◽  
pp. 517-532 ◽  
Author(s):  
Kazuki Kurimoto ◽  
Yukihiro Yabuta ◽  
Katsuhiko Hayashi ◽  
Hiroshi Ohta ◽  
Hiroshi Kiyonari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Chromatin Remodeling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cell Specification ◽  
Germ Cell Specification

Primordial germ cell differentiation of nuclear transfer embryonic stem cells using surface modified electroconductive scaffolds

2016 ◽  
Vol 53 (4) ◽  
pp. 371-380
Author(s):  
Tarlan Eslami-Arshaghi ◽  
Saeid Vakilian ◽  
Ehsan Seyedjafari ◽  
Abdolreza Ardeshirylajimi ◽  
Masoud Soleimani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Nuclear Transfer ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Germ Cell Differentiation ◽  
Surface Modified

scholarly journals Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition

Stem Cells ◽  
2014 ◽  
Vol 32 (10) ◽  
pp. 2668-2678 ◽  
Author(s):  
Tohru Kimura ◽  
Yoshiaki Kaga ◽  
Hiroshi Ohta ◽  
Mika Odamoto ◽  
Yoichi Sekita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Haploid mouse germ cell precursors from embryonic stem cells reveal Xist activation from a single X chromosome

2020 ◽  
Author(s):  
Eishi Aizawa ◽  
Corinne Kaufmann ◽  
Sarah Sting ◽  
Remo Freimann ◽  
Anton Wutz
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
X Chromosome ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Haploid Genome ◽  
Blocking Factors

SummaryMammalian haploid cells have applications for genetic screening and substituting gametic genomes. Here we characterize a culture system for obtaining haploid primordial germ cell-like cells (PCGLCs) from haploid mouse embryonic stem cells (ESCs). We find that a haploid genome is maintained in PGCLCs with a high frequency indicating a substantially lower rate of diploidization than somatic cells. Characterization of the differentiating haploid ESCs reveals that Xist is activated from the single X chromosome. This observation suggests that X chromosome inactivation is initiated in haploid cells consistent with a model where autosomal blocking factors set a threshold for X-linked activators. The germline segregates from the epiblast and differs from somatic lineages in gene expression and epigenetic mechanisms. The ability of primordial germ cells for repressing Xist might contribute to the maintenance of a haploid genome.

Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation

2010 ◽  
Vol 392 (3) ◽  
pp. 311-316 ◽  
Author(s):  
Noriko Yamano ◽  
Tohru Kimura ◽  
Shoko Watanabe-Kushima ◽  
Takashi Shinohara ◽  
Toru Nakano
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Akt Activation
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