Back Cover: Dynamic Imaging of Ostwald Ripening by Environmental Scanning Transmission Electron Microscopy (ChemCatChem 22/2015)

Progress is reported in analytical in situ environmental scanning transmission electron microscopy (ESTEM) for visualizing and analysing in real-time dynamic gas–solid catalyst reactions at the single-atom level under controlled reaction conditions of gas environment and temperature. The recent development of the ESTEM advances the capability of the established ETEM with the detection of fundamental single atoms, and the associated atomic structure of selected solid-state heterogeneous catalysts, in catalytic reactions in their working state. The new data provide improved understanding of dynamic atomic processes and reaction mechanisms, in activity and deactivation, at the fundamental level; and in the chemistry underpinning important technological processes. The benefits of atomic resolution-E(S)TEM to science and technology include new knowledge leading to improved technological processes, reductions in energy requirements and better management of environmental waste. This article is part of a discussion meeting issue ‘Dynamic in situ microscopy relating structure and function’.

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Site Dependent Oxidation and Reduction Mechanisms in Nanoparticles Investigated via Environmental Scanning Transmission Electron Microscopy

Microscopy and Microanalysis ◽

10.1017/s1431927619007736 ◽

2019 ◽

Vol 25 (S2) ◽

pp. 1400-1401

Author(s):

Alec P. LaGrow ◽

David C. Lloyd ◽

Edward D. Boyes ◽

Pratibha L. Gai

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Scanning Transmission Electron Microscopy ◽

Environmental Scanning ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Reduction Mechanisms ◽

Scanning Transmission

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Visualizing the Cu/Cu2O Interface Transition in Nanoparticles with Environmental Scanning Transmission Electron Microscopy

Journal of the American Chemical Society ◽

10.1021/jacs.6b08842 ◽

2016 ◽

Vol 139 (1) ◽

pp. 179-185 ◽

Cited By ~ 65

Author(s):

Alec P. LaGrow ◽

Michael R. Ward ◽

David C. Lloyd ◽

Pratibha L. Gai ◽

Edward D. Boyes

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Scanning Transmission Electron Microscopy ◽

Environmental Scanning ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Scanning Transmission

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The Effect of Electron Beam Irradiation in Environmental Scanning Transmission Electron Microscopy of Whole Cells in Liquid

Microscopy and Microanalysis ◽

10.1017/s1431927616000763 ◽

2016 ◽

Vol 22 (3) ◽

pp. 656-665 ◽

Cited By ~ 21

Author(s):

Justus Hermannsdörfer ◽

Verena Tinnemann ◽

Diana B. Peckys ◽

Niels de Jonge

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Electron Beam ◽

Scanning Transmission Electron Microscopy ◽

Environmental Scanning ◽

Beam Radiation ◽

Whole Cells ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Scanning Transmission

AbstractWhole cells can be studied in their native liquid environment using electron microscopy, and unique information about the locations and stoichiometry of individual membrane proteins can be obtained from many cells thus taking cell heterogeneity into account. Of key importance for the further development of this microscopy technology is knowledge about the effect of electron beam radiation on the samples under investigation. We used environmental scanning electron microscopy (ESEM) with scanning transmission electron microscopy (STEM) detection to examine the effect of radiation for whole fixed COS7 fibroblasts in liquid. The main observation was the localization of nanoparticle labels attached to epidermal growth factor receptors (EGFRs). It was found that the relative distances between the labels remained mostly unchanged (<1.5%) for electron doses ranging from the undamaged native state at 10 e−/Å2 toward 103 e−/Å2. This dose range was sufficient to determine the EGFR locations with nanometer resolution and to distinguish between monomers and dimers. Various different forms of radiation damage became visible at higher doses, including severe dislocation, and the dissolution of labels.

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Scanning Transmission Electron Microscopy of Polyethylene Crystals

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600001069 ◽

1980 ◽

Vol 38 ◽

pp. 242-245

Author(s):

F. Khoury ◽

L. H. Bolz

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Crystallization Temperature ◽

Scanning Transmission Electron Microscopy ◽

Growth Habit ◽

Lateral Growth ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Scanning Transmission ◽

Growth Habits

The lateral growth habits and non-planar conformations of polyethylene crystals grown from dilute solutions (<0.1% wt./vol.) are known to vary depending on the crystallization temperature.1-3 With the notable exception of a study by Keith2, most previous studies have been limited to crystals grown at <95°C. The trend in the change of the lateral growth habit of the crystals with increasing crystallization temperature (other factors remaining equal, i.e. polymer mol. wt. and concentration, solvent) is illustrated in Fig.l. The lateral growth faces in the lozenge shaped type of crystal (Fig.la) which is formed at lower temperatures are {110}. Crystals formed at higher temperatures exhibit 'truncated' profiles (Figs. lb,c) and are bound laterally by (110) and (200} growth faces. In addition, the shape of the latter crystals is all the more truncated (Fig.lc), and hence all the more elongated parallel to the b-axis, the higher the crystallization temperature.

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