ChemInform Abstract: First Bovine Serum Albumin-Promoted Synthesis of Enones, Cinnamic Acids and Coumarins in Ionic Liquid: An Insight into the Role of Protein Impurities in Porcine Pancreas Lipase for Olefinic Bond Formation.

ChemInform ◽  
2011 ◽  
Vol 42 (36) ◽  
pp. no-no
Author(s):  
Nandini Sharma ◽  
Upendra K. Sharma ◽  
Rajesh Kumar ◽  
Nidhi Katoch ◽  
Rakesh Kumar ◽  
...  
ChemCatChem ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 3050-3056 ◽  
Author(s):  
Yogesh Thopate ◽  
Richa Singh ◽  
Arun K. Sinha ◽  
Vikash Kumar ◽  
Mohammad Imran Siddiqi

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2993 ◽  
Author(s):  
José Guimarães ◽  
Raquel Giordano ◽  
Roberto Fernandez-Lafuente ◽  
Paulo Tardioli

The preparation of highly porous magnetic crosslinked aggregates (pm-CLEA) of porcine pancreas lipase (PPL) is reported. Some strategies to improve the volumetric activity of the immobilized biocatalyst were evaluated, such as treatment of PPL with enzyme surface-modifying agents (polyethyleneimine or dodecyl aldehyde), co-aggregation with protein co-feeders (bovine serum albumin and/or soy protein), use of silica magnetic nanoparticles functionalized with amino groups (SMNPs) as separation aid, and starch as pore-making agent. The combination of enzyme surface modification with dodecyl aldehyde, co-aggregation with SMNPs and soy protein, in the presence of 0.8% starch (followed by hydrolysis of the starch with α-amylase), yielded CLEAs expressing high activity (immobilization yield around 100% and recovered activity around 80%), high effectiveness factor (approximately 65% of the equivalent free enzyme activity) and high stability at 40 °C and pH 8.0, i.e., PPL CLEAs co-aggregated with SMNPs/bovine serum albumin or SMNPs/soy protein retained 80% and 50% activity after 10 h incubation, respectively, while free PPL was fully inactivated after 2 h. Besides, highly porous magnetic CLEAs co-aggregated with soy protein and magnetic nanoparticles (pm-SP-CLEAs) showed good performance and reusability in the hydrolysis of tributyrin for five 4h-batches.


2014 ◽  
Vol 27 (5) ◽  
pp. 239-249 ◽  
Author(s):  
Yan Liu ◽  
Mingmao Chen ◽  
Shuaihua Wang ◽  
Jingjing Lin ◽  
Lizhen Cai ◽  
...  

2017 ◽  
Vol 176 ◽  
pp. 75-82 ◽  
Author(s):  
Guo Li ◽  
Jianying Huang ◽  
Tao Chen ◽  
Xiangyang Wang ◽  
Haijiang Zhang ◽  
...  

2020 ◽  
Vol 154 ◽  
pp. 1448-1459 ◽  
Author(s):  
Tajalli Ilm Chandel ◽  
Mariyam Afghani ◽  
Aiman Masroor ◽  
Ibrar Ahmad Siddique ◽  
Syed Mohammad Zakariya ◽  
...  

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