Chiral separation plays a very important role in the modern pharmaceutical analysis and will
continue in upcoming years. Separation and identification of chiral impurities are indispensable. According
to ICH guidelines, only the active enantiomer of the drug has to be marketed, so there is a focus
on separation of the inactive enantiomer which acts as a chiral impurity. The impurities present in the
enantiomers also pose various toxic adverse effects on bioavailability and efficacy, hence the need to
separate these impurities will forever be trending. This review primarily focuses on the separation techniques
like Capillary Electrophoresis (CE), High-Performance Liquid Chromatography (HPLC), Gas
Chromatography (GC), and Supercritical Fluid Chromatography (SFC) followed by the year-wise trend
in the separation of selected chiral impurities. In the coming years, researchers should work on using
ultra-fast, selective, and sensitive methods for the effective separation of chiral impurities.
