How transfer rates generate Gd-BOPTA concentrations in rat liver compartments: implications for clinical liver imaging with hepatobiliary contrast agents

With recent advances in liver imaging, the estimation of liver concentrations is now possible following the injection of hepatobiliary contrast agents and radiotracers. However, how these images are generated remains partially unknown. Most experiments that would be helpful to increase this understanding cannot be performed in vivo. For these reasons, we investigated the liver distribution of the magnetic resonance (MR) contrast agent gadobenate dimeglumine (Gd-BOPTA, MultiHance®, Bracco Imaging) in isolated perfused rat livers (IPRLs). In IPRL, we developed a new set up that quantifies simultaneously the Gd-BOPTA compartment concentrations and the transfer rates between these compartments. Concentrations were measured either by MR signal intensity or by count rates when the contrast agent was labelled by [153Gd]. With this experimental model, we show how the Gd-BOPTA hepatocyte concentrations are modified by temperature and liver flow rates. We define new pharmacokinetic parameters to quantify the canalicular transport of Gd-BOPTA. Finally, we present how transfer rates generate Gd-BOPTA concentrations in rat liver compartments. These findings better explain how liver imaging with hepatobiliary radiotracers and contrast agents is generated and improve the image interpretation by clinicians.

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Liver imaging

Acta Radiologica ◽

10.1080/02841859709172392 ◽

1997 ◽

Vol 38 (5) ◽

pp. 626-630 ◽

Cited By ~ 6

Author(s):

E. J. Rummeny ◽

G. Marchal

Keyword(s):

Contrast Agents ◽

Final Diagnosis ◽

Diagnostic Value ◽

Primary Hepatocellular Carcinoma ◽

Imaging Modalities ◽

Organ Specificity ◽

Liver Imaging ◽

Liver Tumours ◽

Patient Health ◽

Beneficial Impact

Primary hepatocellular carcinoma and liver metastases affect several millon people each year. The main imaging modalities to detect and assist diagnosis of primary and secondary liver tumours include MR imaging, CT, and US. The value of these techniques is further increased by the use of contrast agents which increase the sensitivity, and sometimes also the specificity, of the investigations. The relative advantages and drawbacks of the different contrast agents and imaging modalities in the detection and characterisation of liver tumours are discussed. Currently there is no consensus amongst investigators as to which is superior, due to the technical complexities and number of combinations possible within each of the different modalities. There continues to be advances in the hardware and software of imaging equipment, as well as a trend to develop new contrast agents with more organ-specificity. These include those targeting the hepatocytes, such as mangafodipir trisodium (MnDPDP, Teslascan), and those with reticuloendothelial cell specificity, such as the superpara-magnetic iron oxides. These developments have the potential for making significant contributions to the diagnostic value of imaging procedures and, by reducing the number of investigations necessary to reach a final diagnosis, having a significant and beneficial impact on the pharmaco-economics of patient health care.

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Part 1: MRI features of focal nodular hyperplasia with an emphasis on hepatobiliary contrast agents

Journal of Medical Imaging and Radiation Oncology ◽

10.1111/1754-9485.12130 ◽

2013 ◽

Vol 58 (1) ◽

pp. 50-55 ◽

Cited By ~ 5

Author(s):

Tom Sutherland ◽

Melanie Seale ◽

Kelvin Yap

Keyword(s):

Contrast Agents ◽

Focal Nodular Hyperplasia ◽

Mri Features ◽

Nodular Hyperplasia ◽

Hepatobiliary Contrast Agents

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Dynamic evaluation of the hepatic uptake and clearance of manganese-based MRI contrast agents: A31P NMR study on the isolated and perfused rat liver

Journal of Magnetic Resonance Imaging ◽

10.1002/jmri.1880080322 ◽

1998 ◽

Vol 8 (3) ◽

pp. 663-669 ◽

Cited By ~ 8

Author(s):

Jean-Marie Colet ◽

Luce Vander Elst ◽

Robert N. Muller

Keyword(s):

Contrast Agents ◽

Rat Liver ◽

Hepatic Uptake ◽

Mri Contrast Agents ◽

Perfused Rat Liver ◽

Mri Contrast ◽

Dynamic Evaluation ◽

Nmr Study

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Perbandingan Penyangatan Senyawa Pengontras Nano Partikel Emas Dendrimer Poly (Amidoamine) Konsentrasi dan Delay Time Berbeda pada Hepar Tikus dengan Pemeriksaan Computed Tomography

Jurnal Radiologi Indonesia ◽

10.33748/jradidn.v1i3.17 ◽

2016 ◽

Vol 1 (3) ◽

pp. 131-137

Author(s):

Indrarini Listyowati ◽

Rista D Soetikno ◽

Leni Santiana

Keyword(s):

Computed Tomography ◽

Statistical Analysis ◽

Contrast Agent ◽

Contrast Agents ◽

Ct Scan ◽

Rat Liver ◽

Delay Time ◽

P Value ◽

Average Value ◽

Targeted Contrast Agents

Background: Computed tomography (CT) is one of the diagnostic imaging tool that is most commonly available in hospitals today. But unlike Magnetic Resonance Imaging (MRI) and nuclear medicine imaging modalities, CT has not been considered as a molecular imaging modality because it does not have a specifc-targeted contrast agents. Diagnostic quality of CT scans can be improved by using specifc-targeted contrast agents. Gold nanoparticles (AuNPs) can be used as X-ray contrast agents that can overcome some of the limitations of the iodine-based contrast agents. Higher atomic numbers than iodine and its ability to be able to bind to the dendrimer support that AuNPS could be developed as a specifc-targeted contrast agents.Objectives: The purpose of this study was to determine the enhancement relationship of AuNPs-Poly(amido)amine in the rat liver with di?erent concentration and delay time on CT scans.Materials and Methods: This research is an experimental study with a statistical analysis to determine enhancement di?erences on rat liver given AuNPs-PAMAM with di?erent concentration and delay time. The samples were divided into 4 groups, each consisting of 6 rats. The independent variables in this study were the delay time and the concentration of AuNPs-PAMAM, the dependent variables was the enhancement on rat liver.Results: The results obtained showed that the average value enhancement of AuNPs-PAMAM is highest in 10 minutes delay time at a concentration of 1 mg/dL. Statistical analysis showed that there were signifcant di?erences between AuNPs-PAMAM contrast enhancement in the rat liver with di?erent concentration and delay time on CT scan with p-value less than alpha (0.000 <0.05).Conclusions: Concentration and delay time contribute to the average value enhancement of AuNPs-PAMAM contrast agent on CT scan. This new type of contrast agent can be further developed as a more specifc-targeted contrast agent for CT scan examination.

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