scholarly journals Lack of significant differences in the corrected activity of lysophospholipase D, producer of phospholipid mediator lysophosphatidic acid, in incubated serum from women with and without ovarian tumors

Cancer ◽  
2001 ◽  
Vol 94 (1) ◽  
pp. 141-151 ◽  
Author(s):  
Akira Tokumura ◽  
Kyoko Tominaga ◽  
Katsuhiko Yasuda ◽  
Hideharu Kanzaki ◽  
Kentaro Kogure ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Ovarian Tumors ◽  
Lysophospholipase D

Production of bioactive lysophosphatidic acid by lysophospholipase D in hen egg white

2007 ◽  
Vol 1771 (4) ◽  
pp. 491-499 ◽  
Author(s):  
J MORISHIGE ◽  
K TOUCHIKA ◽  
T TANAKA ◽  
K SATOUCHI ◽  
K FUKUZAWA ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Egg White ◽  
Lysophospholipase D ◽  
Hen Egg White ◽  
Hen Egg

scholarly journals Autotaxin, a Secreted Lysophospholipase D, Is Essential for Blood Vessel Formation during Development

2006 ◽  
Vol 26 (13) ◽  
pp. 5015-5022 ◽  
Author(s):  
Laurens A. van Meeteren ◽  
Paula Ruurs ◽  
Catelijne Stortelers ◽  
Peter Bouwman ◽  
Marga A. van Rooijen ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Critical Role ◽  
G Protein Coupled Receptors ◽  
Blood Vessel Formation ◽  
Lysophospholipase D ◽  
Motility Factor ◽  
Nucleotide Pyrophosphatase ◽  
G Protein Coupled ◽  
Deficient Mice

ABSTRACT Autotaxin (ATX), or nucleotide pyrophosphatase-phosphodiesterase 2, is a secreted lysophospholipase D that promotes cell migration, metastasis, and angiogenesis. ATX generates lysophosphatidic acid (LPA), a lipid mitogen and motility factor that acts on several G protein-coupled receptors. Here we report that ATX-deficient mice die at embryonic day 9.5 (E9.5) with profound vascular defects in yolk sac and embryo resembling the Gα13 knockout phenotype. Furthermore, at E8.5, ATX-deficient embryos showed allantois malformation, neural tube defects, and asymmetric headfolds. The onset of these abnormalities coincided with increased expression of ATX and LPA receptors in normal embryos. ATX heterozygous mice appear healthy but show half-normal ATX activity and plasma LPA levels. Our results reveal a critical role for ATX in vascular development, indicate that ATX is the major LPA-producing enzyme in vivo, and suggest that the vascular defects in ATX-deficient embryos may be explained by loss of LPA signaling through Gα13.

scholarly journals Production of Lysophosphatidic Acid in Blister Fluid: Involvement of a Lysophospholipase D Activity

2005 ◽  
Vol 125 (3) ◽  
pp. 421-427 ◽  
Author(s):  
Juliette Mazereeuw-Hautier ◽  
Sandra Gres ◽  
Madie Fanguin ◽  
Clotilde Cariven ◽  
Josette Fauvel ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Blister Fluid ◽  
Lysophospholipase D

scholarly journals Secretion of a lysophospholipase D activity by adipocytes: involvement in lysophosphatidic acid synthesis

2002 ◽  
Vol 43 (6) ◽  
pp. 904-910 ◽  
Author(s):  
Stéphane Gesta ◽  
Marie-Françoise Simon ◽  
Astrid Rey ◽  
David Sibrac ◽  
Alexia Girard ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Acid Synthesis ◽  
Lysophospholipase D

scholarly journals Increased Production of Bioactive Lysophosphatidic Acid by Serum Lysophospholipase D in Human Pregnancy1

2002 ◽  
Vol 67 (5) ◽  
pp. 1386-1392 ◽  
Author(s):  
Akira Tokumura ◽  
Yumi Kanaya ◽  
Maki Miyake ◽  
Shuji Yamano ◽  
Minoru Irahara ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Lysophospholipase D

scholarly journals Lysophospholipase D activity exists in the urine to catalyse the formation of lysophosphatidic acid

2006 ◽  
Vol 21 (12) ◽  
pp. 3612-3613 ◽  
Author(s):  
R. Ohkawa ◽  
N. Hisano ◽  
K. Nakamura ◽  
S. Okubo ◽  
H. Yokota ◽  
...  
Keyword(s):  
Lysophosphatidic Acid ◽  
Lysophospholipase D

scholarly journals Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production

2002 ◽  
Vol 158 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Makiko Umezu-Goto ◽  
Yasuhiro Kishi ◽  
Akitsu Taira ◽  
Kotaro Hama ◽  
Naoshi Dohmae ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tumor Cell ◽  
Cell Motility ◽  
Cell Lines ◽  
Lysophosphatidic Acid ◽  
Cellular Motility ◽  
Lysophospholipase D ◽  
Nucleotide Pyrophosphatase ◽  
Tumor Cell Motility ◽  
Km Value

Autotaxin (ATX) is a tumor cell motility–stimulating factor, originally isolated from melanoma cell supernatants. ATX had been proposed to mediate its effects through 5′-nucleotide pyrophosphatase and phosphodiesterase activities. However, the ATX substrate mediating the increase in cellular motility remains to be identified. Here, we demonstrated that lysophospholipase D (lysoPLD) purified from fetal bovine serum, which catalyzes the production of the bioactive phospholipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine (LPC), is identical to ATX. The Km value of ATX for LPC was 25-fold lower than that for the synthetic nucleoside substrate, p-nitrophenyl-tri-monophosphate. LPA mediates multiple biological functions including cytoskeletal reorganization, chemotaxis, and cell growth through activation of specific G protein–coupled receptors. Recombinant ATX, particularly in the presence of LPC, dramatically increased chemotaxis and proliferation of multiple different cell lines. Moreover, we demonstrate that several cancer cell lines release significant amounts of LPC, a substrate for ATX, into the culture medium. The demonstration that ATX and lysoPLD are identical suggests that autocrine or paracrine production of LPA contributes to tumor cell motility, survival, and proliferation. It also provides potential novel targets for therapy of pathophysiological states including cancer.

scholarly journals Identification of Human Plasma Lysophospholipase D, a Lysophosphatidic Acid-producing Enzyme, as Autotaxin, a Multifunctional Phosphodiesterase

2002 ◽  
Vol 277 (42) ◽  
pp. 39436-39442 ◽  
Author(s):  
Akira Tokumura ◽  
Eiji Majima ◽  
Yuko Kariya ◽  
Kyoko Tominaga ◽  
Kentaro Kogure ◽  
...  
Keyword(s):  
Human Plasma ◽  
Lysophosphatidic Acid ◽  
Lysophospholipase D
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