Adaptation of Mammalian Cells to Chemically Defined Media

2019 ◽  
Vol 82 (1) ◽  
Author(s):  
Bianca Marigliani ◽  
Luciene Bottentuit López Balottin ◽  
Elisabeth de Fatima Pires Augusto



1972 ◽  
Vol 18 (7) ◽  
pp. 1087-1090 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
R. Wallace ◽  
L. Greenberg

Neisseria Chemically Defined Medium (NCDM) has been used routinely in our laboratory for a variety of purposes. The present report describes the development of NCDM agar, wherein the NCDM base is sterilized by filtration and defined supplements and agar are added. The medium is transparent and both meningococci and gonococci grow within 72 h. When grown on NCDM agar, Types 2 and 3 gonococcal colonies tend to revert to Type 1. The serological grouping of meningococci with specific antisera is not affected by growth on this medium.Parallel investigations on the growth of these species in liquid NCDM demonstrated that the yield of Neisseria gonorrhoeae is enhanced when the medium is sterilized by filtration.







2018 ◽  
Vol 94 (4) ◽  
pp. 1144-1155 ◽  
Author(s):  
Florian Krattenmacher ◽  
Tamara Heermann ◽  
Amandine Calvet ◽  
Bartlomiej Krawczyk ◽  
Thomas Noll


2003 ◽  
Vol 69 (6) ◽  
pp. 2092-2099 ◽  
Author(s):  
Koji Yoshioka ◽  
Chie Suzuki ◽  
Seigo Itoh ◽  
Kazuhiro Kikuchi ◽  
Shokichi Iwamura ◽  
...  


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