scholarly journals Production of Piglets Derived from In Vitro-Produced Blastocysts Fertilized and Cultured in Chemically Defined Media: Effects of Theophylline, Adenosine, and Cysteine During In Vitro Fertilization1

2003 ◽  
Vol 69 (6) ◽  
pp. 2092-2099 ◽  
Author(s):  
Koji Yoshioka ◽  
Chie Suzuki ◽  
Seigo Itoh ◽  
Kazuhiro Kikuchi ◽  
Shokichi Iwamura ◽  
...  

Development ◽  
1975 ◽  
Vol 34 (2) ◽  
pp. 299-310
Author(s):  
P. S. Grant ◽  
I. Ljungkvist ◽  
O. Nilsson

When mature and immature uteri from ovariectomized mice were cultured in chemically defined media, blastocyst invasion occurred in the presence of progesterone but not in media containing only oestradiol. The invaded stromal tissue did not decidualize unless the uteri were taken from mice pretreated with progesterone and oestradiol. Fibrillar material was, however, concentrated in the stromal tissue adjacent to the invasive trophoblast. Neither progesterone nor oestradiol, had any ultrastructural effect on the endometrium of cultured uteri from ovariectomized mice, other than closure of uterine lumina in the presence of progesterone. Embryogenesis became abnormal, probably owing to the failure of formation of a suitable implantation chamber.



2002 ◽  
Vol 69 (3-4) ◽  
pp. 151-158 ◽  
Author(s):  
H.J. Hernandez-Fonseca ◽  
S. Sirisathien ◽  
P. Bosch ◽  
H.S. Cho ◽  
J.D. Lott ◽  
...  




1963 ◽  
Vol 41 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Gloria A. Webster ◽  
T. W. M. Cameron

A variety of media and techniques has been employed in attempts to grow various stages of Echinococcus in vitro. Whole, intact Echinococcus multilocularis cysts and undifferentiated cystic material proliferate readily in chemically defined media, but except for a single occurrence, no scoleces developed within the new vesicles. Scoleces can be induced to develop either toward the tapeworm stage or the vesicular larval stage by altering the medium. In the first case, the scoleces increase in size, the excretory system becomes prominent, and segmentation is advanced; gonads have not developed in any medium yet employed. Scoleces may vesiculate in several ways; ultimately, they become vacuolated, the suckers are absorbed, the hooks shed, and a thin, laminated membrane is laid down. Local internal thickenings probably represent secondary brood capsules or scoleces.



Nature ◽  
1972 ◽  
Vol 238 (5362) ◽  
pp. 270-271 ◽  
Author(s):  
SHYOSO OGAWA ◽  
KAHEI SATOH ◽  
MITSUMA HAMADA ◽  
HAJIME HASHIMOTO




2019 ◽  
Vol 13 (1) ◽  
pp. 221-234 ◽  
Author(s):  
Kwang-Hwan Choi ◽  
Dong-Kyung Lee ◽  
Sung Woo Kim ◽  
Sang-Ho Woo ◽  
Dae-Yong Kim ◽  
...  


1996 ◽  
Vol 70 (2) ◽  
pp. 173-175 ◽  
Author(s):  
H. Hata

AbstractThird-stage larvae of Angiostrongylus costaricensis were cultured to young adult stages in Waymouth's chemically defined medium MB 752/l, which comprises higher concentrations of the essential components histidine, lysine, methionine, tryptophan, choline chloride and glucose than various other chemically defined media. The present study has shown that choline chloride and tryptophan are required at relatively higher concentrations for worm development than those of the other essential components.



2009 ◽  
Vol 42 (3) ◽  
Author(s):  
HONG-SHENG WU ◽  
YANG WANG ◽  
CHAO-YONG ZHANG ◽  
WEI BAO ◽  
NING LING ◽  
...  


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