scholarly journals Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging

2020 ◽  
Vol 97 (5) ◽  
pp. 515-527 ◽  
Author(s):  
Asylkhan Rakhymzhan ◽  
Lucie Reuter ◽  
Raphael Raspe ◽  
Daniel Bremer ◽  
Robert Günther ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
Spectral Optical Coherence Tomography

scholarly journals Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn
Keyword(s):  
Wavelength Region ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Deep Tissue Imaging

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

scholarly journals Advantages of Non-degenerate Two-photon Microscopy for Deep Tissue Imaging

2020 ◽  
Vol 118 (3) ◽  
pp. 311a
Author(s):  
Sanaz Sadegh ◽  
Mu-Han Yang ◽  
Christopher Ferri ◽  
Martin Thunemann ◽  
Anna Devor ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals Dual-axis optical coherence tomography for deep tissue imaging

2017 ◽  
Vol 42 (12) ◽  
pp. 2302 ◽  
Author(s):  
Yang Zhao ◽  
Will J. Eldridge ◽  
Jason R. Maher ◽  
Sanghoon Kim ◽  
Michael Crose ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Deep Tissue ◽  
Deep Tissue Imaging

scholarly journals A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

2015 ◽  
Vol 6 (3) ◽  
pp. 1944-1948 ◽  
Author(s):  
Pablo Rivera-Fuentes ◽  
Alexandra T. Wrobel ◽  
Melissa L. Zastrow ◽  
Mustafa Khan ◽  
John Georgiou ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Live Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
Acidic Vesicles ◽  
Unambiguous Detection

A fluorescent sensor that is pH-insensitive and compatible with two-photon microscopy was developed and applied to live cell and deep tissue imaging.

Does digital deconvolution improve two-photon microscopy in deep tissue imaging?

2001 ◽  
Author(s):  
Colten R. Noakes ◽  
Toshiyasu Goto ◽  
Raymond Keller ◽  
Ammasi Periasamy
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

Two-Photon Microscopy for Deep Tissue Imaging of Living Specimens

2012 ◽  
Vol 20 (4) ◽  
pp. 12-16
Author(s):  
Tilman Franke ◽  
Sebastian Rhode
Keyword(s):  
Three Dimensional ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Time Resolved ◽  
3D Images ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
And Function ◽  
Structural Contrast

Two-photon microscopy (2PM) provides three-dimensional (3D) and four-dimensional (4D) (x, y, z, t) imaging in living specimens or under experimental physiological conditions very close to live. In conjunction with fluorescent labels, 2PM provides a powerful means of investigating the relationships between structure and function at the microscopic level that are key to understanding biological systems. This technique is able to provide time-resolved, 3D images of dynamic systems with near-diffraction-limited resolution and highly specific structural contrast.

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