scholarly journals Current international flow cytometric practices for the detection and monitoring of paroxysmal nocturnal haemoglobinuria clones: A UK NEQAS survey

2016 ◽  
Vol 92 (4) ◽  
pp. 266-274 ◽  
Author(s):  
Matthew Fletcher ◽  
Liam Whitby ◽  
Alison Whitby ◽  
David Barnett
Keyword(s):  
Paroxysmal Nocturnal Haemoglobinuria ◽  
International Flow ◽  
Flow Cytometric

scholarly journals Paroxysmal Nocturnal Haemoglobinuria - An Atypical Presentation

2019 ◽  
Vol 20 (1) ◽  
pp. 46-48
Author(s):  
Tahmina Rahman ◽  
Mostofa Kamal Chowdhury
Keyword(s):  
Stem Cell ◽  
Venous Thrombosis ◽  
Aplastic Anaemia ◽  
Flow Cytometric Analysis ◽  
Paroxysmal Nocturnal Haemoglobinuria ◽  
Atypical Presentation ◽  
Oral Ulceration ◽  
Flow Cytometric ◽  
Laboratory Features ◽  
History Of

Paroxysmal Nocturnal Haemoglobinuria (PNH) is a rare, acquired disorder of haemopoitic stem cell, characterized by abnormal sensitivity of red cells to haemolytic action of complement leading to intravascular haemolysis. Though PNH typically presents as nocturnal haemoglobinuria, however presentation varies as for example bicytopenia, aplastic anaemia or repeated venous thrombosis. One such atypical presentation was seen in 35 years old male who presented with the history of generalized weakness, fever and oral ulceration. The PNH was diagnosed by flow cytometric analysis of GPI linked protein and associated laboratory features of chronic haemolysis. We report this case to create awareness about the fact that PNH can present without haemoglobinuria. J MEDICINE JUL 2019; 20 (1) : 46-48

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells

Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

1994 ◽  
Vol 72 (05) ◽  
pp. 762-769 ◽  
Author(s):  
Toshiro Takafuta ◽  
Kingo Fujirmura ◽  
Hironori Kawano ◽  
Masaaki Noda ◽  
Tetsuro Fujimoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Specific Protein ◽  
Platelet Membrane ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Flow Cytometric ◽  
Polymerase Chain ◽  
Megakaryocytic Cell

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

Sign in / Sign up

Export Citation Format

Share Document