scholarly journals Organ‐on‐a‐chip: Determine feasibility of a human liver microphysiological model to assess long‐term steroid metabolites in sports drug testing

2021 ◽  
Author(s):  
Christian Görgens ◽  
Anja Patricia Ramme ◽  
Sven Guddat ◽  
Yvonne Schrader ◽  
Annika Winter ◽  
...  
Keyword(s):  
Human Liver ◽  
Drug Testing ◽  
Sports Drug Testing ◽  
Organ On A Chip

Synthesis, characterization, and detection of new oxandrolone metabolites as long-term markers in sports drug testing

2013 ◽  
Vol 405 (25) ◽  
pp. 8285-8294 ◽  
Author(s):  
S. Guddat ◽  
G. Fußhöller ◽  
S. Beuck ◽  
A. Thomas ◽  
H. Geyer ◽  
...  
Keyword(s):  
Drug Testing ◽  
Sports Drug Testing

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System

scholarly journals Thermoplastic Elastomer (TPE)–Poly(Methyl Methacrylate) (PMMA) Hybrid Devices for Active Pumping PDMS-Free Organ-on-a-Chip Systems

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 162
Author(s):  
Mathias Busek ◽  
Steffen Nøvik ◽  
Aleksandra Aizenshtadt ◽  
Mikel Amirola-Martinez ◽  
Thomas Combriat ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Drug Testing ◽  
Low Cost ◽  
Thermoplastic Elastomer ◽  
Bonding Process ◽  
Cell Alignment ◽  
Poly Methyl Methacrylate ◽  
Bonding Parameters ◽  
Organ On A Chip ◽  
Hybrid Devices

Polydimethylsiloxane (PDMS) has been used in microfluidic systems for years, as it can be easily structured and its flexibility makes it easy to integrate actuators including pneumatic pumps. In addition, the good optical properties of the material are well suited for analytical systems. In addition to its positive aspects, PDMS is well known to adsorb small molecules, which limits its usability when it comes to drug testing, e.g., in organ-on-a-chip (OoC) systems. Therefore, alternatives to PDMS are in high demand. In this study, we use thermoplastic elastomer (TPE) films thermally bonded to laser-cut poly(methyl methacrylate) (PMMA) sheets to build up multilayered microfluidic devices with integrated pneumatic micro-pumps. We present a low-cost manufacturing technology based on a conventional CO2 laser cutter for structuring, a spin-coating process for TPE film fabrication, and a thermal bonding process using a pneumatic hot-press. UV treatment with an Excimer lamp prior to bonding drastically improves the bonding process. Optimized bonding parameters were characterized by measuring the burst load upon applying pressure and via profilometer-based measurement of channel deformation. Next, flow and long-term stability of the chip layout were measured using microparticle Image Velocimetry (uPIV). Finally, human endothelial cells were seeded in the microchannels to check biocompatibility and flow-directed cell alignment. The presented device is compatible with a real-time live-cell analysis system.

scholarly journals Paper spray mass spectrometry – A potential complementary technique for the detection of polar compounds in sports drug testing

2020 ◽  
Vol 12 (11-12) ◽  
pp. 1658-1665 ◽  
Author(s):  
Christian Görgens ◽  
Katherine Walker ◽  
Cornelia Boeser ◽  
Neloni Wijeratne ◽  
Claudia Martins ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Testing ◽  
Polar Compounds ◽  
Paper Spray ◽  
Complementary Technique ◽  
Sports Drug Testing

Annual banned-substance review: analytical approaches in human sports drug testing

2014 ◽  
Vol 7 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Mario Thevis ◽  
Tiia Kuuranne ◽  
Hans Geyer ◽  
Wilhelm Schänzer
Keyword(s):  
Drug Testing ◽  
Sports Drug Testing ◽  
Analytical Approaches

Synthesis, characterisation, and mass spectrometric detection of a pegylated EPO-mimetic peptide for sports drug testing purposes

2011 ◽  
Vol 25 (15) ◽  
pp. 2115-2123 ◽  
Author(s):  
Ines Möller ◽  
Andreas Thomas ◽  
Hans Geyer ◽  
Wilhelm Schänzer ◽  
Mario Thevis
Keyword(s):  
Drug Testing ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Mimetic Peptide ◽  
Sports Drug Testing

Analytical Approaches in Human Sports Drug Testing: Recent Advances, Challenges, and Solutions

2019 ◽  
Vol 92 (1) ◽  
pp. 506-523 ◽  
Author(s):  
Mario Thevis ◽  
Katja Walpurgis ◽  
Andreas Thomas
Keyword(s):  
Drug Testing ◽  
Recent Advances ◽  
Sports Drug Testing ◽  
Analytical Approaches

Mass Spectrometry in Sports Drug Testing

2010 ◽  
Author(s):  
Mario Thevis
Keyword(s):  
Mass Spectrometry ◽  
Drug Testing ◽  
Sports Drug Testing

Sports drug testing

1997 ◽  
Vol 16 (7) ◽  
pp. VI-VII ◽  
Author(s):  
Jordi Segura ◽  
Peter Hemmersbach ◽  
Peter Sönksen
Keyword(s):  
Drug Testing ◽  
Sports Drug Testing
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