scholarly journals Dynamic GATA6 expression in primitive endoderm formation and maturation in early mouse embryogenesis

2008 ◽  
Vol 237 (10) ◽  
pp. 2820-2829 ◽  
Author(s):  
Kathy Q. Cai ◽  
Callinice D. Capo-Chichi ◽  
Malgorzata E. Rula ◽  
Dong-Hua Yang ◽  
Xiang-Xi Xu
Keyword(s):  
Primitive Endoderm ◽  
Mouse Embryogenesis ◽  
Early Mouse

Visualization of insulin receptors on mouse pre-embryos

1991 ◽  
Vol 3 (1) ◽  
pp. 9 ◽  
Author(s):  
MB Harvey ◽  
PL Kaye
Keyword(s):  
Protein Synthesis ◽  
Insulin Receptor ◽  
Protein Metabolism ◽  
Insulin Receptors ◽  
Mouse Embryos ◽  
Cell Populations ◽  
Mouse Embryogenesis ◽  
Functional Studies ◽  
Early Mouse ◽  
Regulatory Actions

Because insulin stimulates pre-embryonic protein metabolism and growth, the presence of insulin receptors on early mouse embryos was investigated immunohistochemically, using a specific anti-insulin receptor IgG. Staining was not present on fertilized eggs or on 2-cell, 4-cell or uncompacted 8-cell embryos, but insulin receptors were visible on compacting 8-cell embryos and on morulae and blastocysts. This ontogeny correlates with functional studies showing that insulin affects protein synthesis during these post-compaction stages. Insulin receptors were also present on isolated inner cell masses, which have also been shown to be responsive to insulin. Because the ontogeny of the appearance of insulin receptors and the presence of these receptors on both cell populations in the blastocyst coincide with the stimulatory effects of insulin observed in previously reported functional studies on pre-embryos, we believe that these insulin receptors mediate insulin's regulatory actions during early mouse embryogenesis.

scholarly journals The Orphan Steroid Receptor Nur77 Family Member Nor-1 Is Essential for Early Mouse Embryogenesis

2003 ◽  
Vol 278 (47) ◽  
pp. 47104-47109 ◽  
Author(s):  
R. Andrea DeYoung ◽  
Julie C. Baker ◽  
Dragana Cado ◽  
Astar Winoto
Keyword(s):  
Family Member ◽  
Steroid Receptor ◽  
Mouse Embryogenesis ◽  
Early Mouse

scholarly journals Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development

2012 ◽  
Vol 26 (13) ◽  
pp. 1445-1458 ◽  
Author(s):  
F. Lavial ◽  
S. Bessonnard ◽  
Y. Ohnishi ◽  
A. Tsumura ◽  
A. Chandrashekran ◽  
...  
Keyword(s):  
Primitive Endoderm ◽  
Mouse Development ◽  
Early Mouse

scholarly journals Parental imprinting on the mouse X chromosome: effects on the early development of X0, XXY and XXX embryos

1993 ◽  
Vol 62 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Takashi Tada ◽  
Nobuo Takagi ◽  
Ilse-Dore Adler
Keyword(s):  
X Chromosome ◽  
Cytogenetic Study ◽  
Abnormal Development ◽  
Mouse Strains ◽  
Mouse Embryogenesis ◽  
Linked Gene ◽  
Parental Imprinting ◽  
Early Mouse ◽  
The One ◽  
Chromosome Imprinting

SummaryTo examine the effects of X-chromosome imprinting during early mouse embryogenesis, we attempted to produce XM0, Xp0, XMXMY, XMXPY and XMXMXP (where XM and Xp stand for the maternally and the paternally derived X chromosome, respectively) making use of mouse strains bearing the translocation Rb(X.2)2Ad and the inversion In(X)1H. Unlike XMXPY embryos, XMXMY and XMXMXP conceptuses suffered from severe growth retardation or abnormal development characterized by deficient extra-embryonic structures at 6.5–7.5 days post coitum (dpc). A cytogenetic study suggested that two XM chromosomes remaining active in certain non-epiblast cells were responsible for the serious developmental abnormality found in these embryos disomic for XM. Although matings involving females heterozygous for Rb(X.2)2Ad hinted at the paucity of XP0 embryos relative to those having the complementary karyotype of XMXMXP, further study of embryos from matings between females heterozygous for In(X)1H and Rb2Ad males did not substantiate this observation. Thus, the extensive peri-implantation loss of XP0 embryos shown by Hunt (1991) may be confined to XO mothers. Taken together, this study failed to reveal a parentally imprinted X-linked gene essential for early mouse embryogenesis other than the one most probably corresponding to the X-chromosome inactivation centre.

scholarly journals ECM-integrin signalling instructs cellular position-sensing to pattern the early mouse embryo

Development ◽  
2021 ◽  
Author(s):  
Esther Jeong Yoon Kim ◽  
Lydia Sorokin ◽  
Takashi Hiiragi
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Spatial Arrangement ◽  
Cell Types ◽  
Embryonic Cells ◽  
Primitive Endoderm ◽  
Integrin Β1 ◽  
Early Mouse Embryo ◽  
Position Sensing ◽  
Early Mouse

Development entails patterned emergence of diverse cell types within the embryo. In mammals, cells positioned inside the embryo give rise to the inner cell mass (ICM) that eventually forms the embryo proper. Yet the molecular basis of how these cells recognise their ‘inside’ position to instruct their fate is unknown. Here we show that provision of extracellular matrix (ECM) to isolated embryonic cells induces ICM specification and alters subsequent spatial arrangement between epiblast (EPI) and primitive endoderm (PrE) cells that emerge within the ICM. Notably, this effect is dependent on integrin β1 activity and involves apical to basal conversion of cell polarity. We demonstrate that ECM-integrin activity is sufficient for ‘inside’ positional signalling and it is required for proper EPI/PrE patterning. Our findings thus highlight the significance of ECM-integrin adhesion in enabling position-sensing by cells to achieve tissue patterning.

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