Characterization of three synuclein genes in Xenopus laevis

2011 ◽  
Vol 240 (8) ◽  
pp. 2028-2033 ◽  
Author(s):  
Chengdong Wang ◽  
Yao Liu ◽  
Wood Yee Chan ◽  
Sun On Chan ◽  
Horst Grunz ◽  
...  
Keyword(s):  
Xenopus Laevis

scholarly journals Cloning and characterization of an endothelin-3 specific receptor (ETC receptor) from Xenopus laevis dermal melanophores.

1993 ◽  
Vol 268 (25) ◽  
pp. 19126-19133 ◽  
Author(s):  
S. Karne ◽  
C.K. Jayawickreme ◽  
M.R. Lerner
Keyword(s):  
Xenopus Laevis ◽  
Specific Receptor ◽  
Endothelin 3

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal stem cells in Xenopus laevis

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Xenopus Laevis ◽  
Isolation And Characterization

Isolation and characterization of xnov, a Xenopus laevis ortholog of the chicken nov gene

Gene ◽  
1996 ◽  
Vol 171 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Zhentu Ying ◽  
Mary Lou King
Keyword(s):  
Xenopus Laevis ◽  
Isolation And Characterization

scholarly journals O-glycan variability of egg-jelly mucins from Xenopus laevis: characterization of four phenotypes that differ by the terminal glycosylation of their mucins

2000 ◽  
Vol 352 (2) ◽  
pp. 449 ◽  
Author(s):  
Yann GUERARDEL ◽  
Ossarath KOL ◽  
Emmanuel MAES ◽  
Tony LEFEBVRE ◽  
Bénoni BOILLY ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Egg Jelly

scholarly journals Functional Characterization of the Oxantel-Sensitive Acetylcholine Receptor from Trichuris muris

2021 ◽  
Vol 14 (7) ◽  
pp. 698
Author(s):  
Tina V. A. Hansen ◽  
Richard K. Grencis ◽  
Mohamed Issouf ◽  
Cédric Neveu ◽  
Claude L. Charvet
Keyword(s):  
Single Dose ◽  
Mouse Model ◽  
Xenopus Laevis ◽  
Acetylcholine Receptor ◽  
Drug Target ◽  
Functional Characterization ◽  
Xenopus Laevis Oocytes ◽  
Trichuris Muris ◽  
Electrode Voltage

The human whipworm, Trichuris trichiura, is estimated to infect 289.6 million people globally. Control of human trichuriasis is a particular challenge, as most anthelmintics have a limited single-dose efficacy, with the striking exception of the narrow-spectrum anthelmintic, oxantel. We recently identified a novel ACR-16-like subunit from the pig whipworm, T. suis which gave rise to a functional acetylcholine receptor (nAChR) preferentially activated by oxantel. However, there is no ion channel described in the mouse model parasite T. muris so far. Here, we have identified the ACR-16-like and ACR-19 subunits from T. muris, and performed the functional characterization of the receptors in Xenopus laevis oocytes using two-electrode voltage-clamp electrophysiology. We found that the ACR-16-like subunit from T. muris formed a homomeric receptor gated by acetylcholine whereas the ACR-19 failed to create a functional channel. The subsequent pharmacological analysis of the Tmu-ACR-16-like receptor revealed that acetylcholine and oxantel were equally potent. The Tmu-ACR-16-like was more responsive to the toxic agonist epibatidine, but insensitive to pyrantel, in contrast to the Tsu-ACR-16-like receptor. These findings confirm that the ACR-16-like nAChR from Trichuris spp. is a preferential drug target for oxantel, and highlights the pharmacological difference between Trichuris species.

Tx1: a transposable element from Xenopus laevis with some unusual properties

1986 ◽  
Vol 6 (3) ◽  
pp. 933-941
Author(s):  
J E Garrett ◽  
D Carroll
Keyword(s):  
Xenopus Laevis ◽  
Germ Line ◽  
Variable Number ◽  
Target Site ◽  
Nucleotide Sequence Analysis ◽  
Chromosomal Locus ◽  
Stem Loop ◽  
Left Handed ◽  
Transposable Genetic Elements

A family of transposable genetic elements in the genome of the frog, Xenopus laevis, is described. They are designated Tx1. Transposability of the elements was deduced by characterization of a chromosomal locus which is polymorphic for the presence or absence of a Tx1 element. Nucleotide sequence analysis suggested that Tx1 elements show target site specificity, as they are inserted at the pentanucleotide TTTAA in all four cases that were examined. The elements appear to have 19-base-pair (bp) inverted terminal repeats, and they are flanked by 4-bp target duplications (TTAA), although the possibility that they do not create target site duplications is discussed. Tx1 elements have several unusual characteristics: the central portion of each element is comprised of a variable number of two types of 393-bp repeating units; the rightmost 1,000 bp of the element contains separate regions potentially capable of forming bends, left-handed Z-form DNA, and alternative stem-loop structures. Comparisons among single frogs suggest that germ line transposition is relatively infrequent and that variations in numbers of internal repeats accumulate quite slowly at any locus.

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