Septin1 is required for spindle assembly and chromosome congression in mouse oocytes

2011 ◽  
Vol 240 (10) ◽  
pp. 2281-2289 ◽  
Author(s):  
Jinliang Zhu ◽  
Shu-Tao Qi ◽  
Ya-Peng Wang ◽  
Zhen-Bo Wang ◽  
Ying-Chun Ouyang ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocytes ◽  
Chromosome Congression

scholarly journals Septin2 is modified by SUMOylation and required for chromosome congression in mouse oocytes

Cell Cycle ◽  
2010 ◽  
Vol 9 (8) ◽  
pp. 1607-1616 ◽  
Author(s):  
Jin-Liang Zhu ◽  
Sheng-Li Lin ◽  
Mo Li ◽  
Ying-Chun Ouyang ◽  
Yi Hou ◽  
...  
Keyword(s):  
Mouse Oocytes ◽  
Chromosome Congression

scholarly journals Melatonin protects mouse oocyte from heat stress damage

2020 ◽  
Vol 6 (3) ◽  
pp. 13
Author(s):  
Haiyan Du ◽  
Shouhong Wang ◽  
Weiwei Huang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Spindle Assembly ◽  
Shock Group ◽  
Mouse Oocyte ◽  
Control Group ◽  
Mouse Oocytes ◽  
Early Apoptosis ◽  
Maturation Rate

Objective: To explore the potential effect of melatonin on the in-vitro maturation of mouse oocytes under heat shock condition.Methods: This study used a heat shock model of mouse oocyte maturation. The oocytes were randomly divided into three groups: control group, heat shock group and heat shock + melatonin group, in order to evaluate the effect of 1×10−9 mol/L melatonin on the quality of oocytes after heat shock.Results: In comparison with the control group, the maturation rate of mouse oocytes in heat shock group was significantly decreased [(33.00 ± 0.07)% vs. (85.00 ± 0.03)%, p < .01], with abnormal spindle assembly, and the early apoptosis rate was significantly increased [(59.7 ± 4.5)% vs. (22.0 ± 3.5)%, p < .01]. Compared with heat shock group, the maturation rate ofoocytes was significantly increased in heat shock + melatonin group [(70.00 ± 0.05)% vs. (33.00 ± 0.07)%, p < .01], meanwhile, the spindle abnormality rate and the early apoptosis rate were significantly decreased accordingly [(37.3 ± 6.1)% vs. (59.7 ± 4.5)%, p < .01]. The expression level of heat shock protein 70 was significantly up-regulated in heat shock + melatonin group in comparison with other two groups (p < .01).Conclusions: By regulating the over-expression of heat shock protein 70, melatonin can improve the declined maturation rate of oocytes and the increased rates of spindle assembly abnormality and early apoptosis caused by heat shock.

scholarly journals Ran GTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
David Drutovic ◽  
Xing Duan ◽  
Rong Li ◽  
Petr Kalab ◽  
Petr Solc
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocytes ◽  
Ran Gtp ◽  
And Function ◽  
Meiosis I

scholarly journals DNA damage induces a meiotic arrest in mouse oocytes mediated by the spindle assembly checkpoint

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Josie K. Collins ◽  
Simon I. R. Lane ◽  
Julie A. Merriman ◽  
Keith T. Jones
Keyword(s):  
Dna Damage ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Meiotic Arrest ◽  
Mouse Oocytes

scholarly journals Aurora B Phosphorylates Multiple Sites on Mitotic Centromere-associated Kinesin to Spatially and Temporally Regulate Its Function

2007 ◽  
Vol 18 (9) ◽  
pp. 3264-3276 ◽  
Author(s):  
Xin Zhang ◽  
Weijie Lan ◽  
Stephanie C. Ems-McClung ◽  
P. Todd Stukenberg ◽  
Claire E. Walczak
Keyword(s):  
Spindle Assembly ◽  
Aurora B ◽  
Phosphorylation Sites ◽  
Aurora B Kinase ◽  
Chromosome Congression ◽  
Chromosome Arm

Chromosome congression and segregation require the proper attachment of microtubules to the two sister kinetochores. Disruption of either Aurora B kinase or the Kinesin-13 mitotic centromere-associated kinesin (MCAK) increases chromosome misalignment and missegregation due to improper kinetochore–microtubule attachments. MCAK localization and activity are regulated by Aurora B, but how Aurora B phosphorylation of MCAK affects spindle assembly is unclear. Here, we show that the binding of MCAK to chromosome arms is also regulated by Aurora B and that Aurora B-dependent chromosome arm and centromere localization is regulated by distinct two-site phosphoregulatory mechanisms. MCAK association with chromosome arms is promoted by phosphorylation of T95 on MCAK, whereas phosphorylation of S196 on MCAK promotes dissociation from the arms. Although targeting of MCAK to centromeres requires phosphorylation of S110 on MCAK, dephosphorylation of T95 on MCAK increases the binding of MCAK to centromeres. Our study reveals a new role for Aurora B, which is to prevent excess MCAK binding to chromatin to facilitate chromatin-nucleated spindle assembly. Our study also shows that the interplay between multiple phosphorylation sites of MCAK may be critical to temporally and spatially control MCAK function.

scholarly journals Xorbit/CLASP links dynamic microtubules to chromosomes in the Xenopus meiotic spindle

2006 ◽  
Vol 172 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Eva Hannak ◽  
Rebecca Heald
Keyword(s):  
Spindle Assembly ◽  
Dominant Negative ◽  
Meiotic Spindle ◽  
Chromosome Congression ◽  
Biochemical Interaction ◽  
Egg Extracts ◽  
Important Player ◽  
Centromeric Protein ◽  
And Function ◽  
Functional Mechanisms

A family of microtubule (MT)-binding proteins, Orbit/multiple asters/cytoplasmic linker protein–associated protein, has emerged as an important player during mitosis, but their functional mechanisms are poorly understood. In this study, we used meiotic egg extracts to gain insight into the role of the Xenopus laevis homologue Xorbit in spindle assembly and function. Xorbit immunodepletion or its inhibition by a dominant-negative fragment resulted in chromosome alignment defects and aberrant MT structures, including monopolar and small spindles. Xorbit-depleted extracts failed to nucleate MTs around chromatin-coated beads, indicating its essential requirement for spindle assembly in the absence of centrosomes and kinetochores. Xorbit's MT stabilizing effect was most apparent during anaphase, when spindle MTs depolymerized rapidly upon Xorbit inhibition. Biochemical interaction between a COOH-terminal Xorbit fragment and the kinetochore-associated kinesin centromeric protein E may contribute to Xorbit's role in chromosome congression. We propose that Xorbit tethers dynamic MT plus ends to kinetochores and chromatin, providing a stabilizing activity that is crucial for spindle assembly and chromosome segregation.

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