scholarly journals The assembly of caprine Y chromosome sequence reveals a unique paternal phylogenetic pattern and improves our understanding of the origin of domestic goat

2021 ◽  
Author(s):  
Changyi Xiao ◽  
Jingjin Li ◽  
Tanghui Xie ◽  
Jianhai Chen ◽  
Sijia Zhang ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Domestic Goat ◽  
Phylogenetic Pattern ◽  
Chromosome Sequence

263 LABELING BOVINE SPERM Y-CHROMOSOME SEQUENCE USING DNA MIMICS

2009 ◽  
Vol 21 (1) ◽  
pp. 229 ◽  
Author(s):  
B. A. Didion ◽  
R. Bleher
Keyword(s):  
Y Chromosome ◽  
Somatic Cells ◽  
Cell Nuclei ◽  
Metaphase Chromosomes ◽  
Synthetic Dna ◽  
Bovine Sperm ◽  
Hybridization Probes ◽  
Y Chromosomes ◽  
Chromosome Sequence ◽  
In Situ Detection

Flow cytometric separation of X- and Y-chromosome bearing bovine sperm is an accepted technology for use at the commercial level. Nevertheless it is important to continue researching the area of gender-preselected sperm for improved efficiencies. We used a synthetic DNA mimic conjugated to a fluorescent dye for in situ detection of Y chromosomes in metaphase preparations of bovine somatic cells and spermatozoa. Peptide nucleic acids (PNA) are a type of DNA mimic having a higher affinity and stability than conventional DNA probes and are used as hybridization probes to complementary DNA. Using male bovine somatic cells and the Y-chromosome as a template, we arranged for the synthesis of a CY3-conjugated PNA to bind 13 to 15 base pairs of unique, Y-chromosome sequence. By testing different labeling conditions, we found that brief incubation (~1 h) of metaphase chromosomes with the PNA produced a localized signal on the Y-chromosome. No signals were observed when chromosomes of female bovine somatic cells were incubated with the same PNA probe. Because chromosomes occupy non-random territories in all cell nuclei, including sperm, we proposed to find centrally-located signals in 50% of fixed bovine sperm when treated with the same PNA as used for the somatic cells. As expected, we found the PNA signals present in 50% sperm (23/43) existing as a single, centrally-located, round fluorescent dot in the sperm head. Validation studies were also conducted using bovine sperm previously flow sorted into X or Y populations, and we found the signals in accordance to an expected signal present using the PNA (146/165 or 88.5% with PNA signal in presorted Y sperm heads and 13/174 or 7.5% with PNA signal in presorted X sperm heads).

scholarly journals The Red Fox Y-Chromosome in Comparative Context

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 409 ◽  
Author(s):  
Halie M. Rando ◽  
William H. Wadlington ◽  
Jennifer L. Johnson ◽  
Jeremy T. Stutchman ◽  
Lyudmila N. Trut ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Canis Lupus ◽  
Related Species ◽  
Red Fox ◽  
Draft Genome ◽  
Canis Lupus Familiaris ◽  
Short Read ◽  
Repeat Content ◽  
Y Chromosomes ◽  
Chromosome Sequence

While the number of mammalian genome assemblies has proliferated, Y-chromosome assemblies have lagged behind. This discrepancy is caused by biological features of the Y-chromosome, such as its high repeat content, that present challenges to assembly with short-read, next-generation sequencing technologies. Partial Y-chromosome assemblies have been developed for the cat (Felis catus), dog (Canis lupus familiaris), and grey wolf (Canis lupus lupus), providing the opportunity to examine the red fox (Vulpes vulpes) Y-chromosome in the context of closely related species. Here we present a data-driven approach to identifying Y-chromosome sequence among the scaffolds that comprise the short-read assembled red fox genome. First, scaffolds containing genes found on the Y-chromosomes of cats, dogs, and wolves were identified. Next, analysis of the resequenced genomes of 15 male and 15 female foxes revealed scaffolds containing male-specific k-mers and patterns of inter-sex copy number variation consistent with the heterogametic chromosome. Analyzing variation across these two metrics revealed 171 scaffolds containing 3.37 Mbp of putative Y-chromosome sequence. The gene content of these scaffolds is consistent overall with that of the Y-chromosome in other carnivore species, though the red fox Y-chromosome carries more copies of BCORY2 and UBE1Y than has been reported in related species and fewer copies of SRY than in other canids. The assignment of these scaffolds to the Y-chromosome serves to further characterize the content of the red fox draft genome while providing resources for future analyses of canid Y-chromosome evolution.

Y chromosome sequence completed

Nature ◽  
2003 ◽  
Author(s):  
John Whitfield
Keyword(s):  
Y Chromosome ◽  
Chromosome Sequence

DEVELOPMENT OF A SYSTEM FOR MONITORING AND PRESERVATION OF GENETIC RESOURCES ON THE EXAMPLE OF ABORIGENOUS GOATS OF THE ALTAI MOUNTAINS OF MONGOLIA

2021 ◽  
Vol 1 (19) ◽  
pp. 347-349
Author(s):  
V.N. Voronkova ◽  
S.V. Beketov ◽  
Yu.A. Stolpovsky
Keyword(s):  
Population Structure ◽  
Mitochondrial Genome ◽  
Genetic Resources ◽  
Y Chromosome ◽  
Altai Mountains ◽  
Domestic Goat ◽  
Mongolian Altai ◽  
Genetic Risks ◽  
Fundamental Biology

The origin of genetic resources domesticated by a species of animals, their importance in the development of fundamental biology and economics is considered on the example of a domestic goat. The data on genetic risks and population structure of local goat breeds inhabiting the territory of Mongolian Altai, obtained by analyzing SNP genotypes, microsatellite sequences, as well as the mitochondrial genome and genes of the Y chromosome, are presented.

Genomic integrity of the Y chromosome sequence-tagged-sites in infertile and Down syndrome Jordanian males

Andrologia ◽  
2013 ◽  
Vol 46 (7) ◽  
pp. 770-776 ◽  
Author(s):  
S. R. Yasin ◽  
L. H. Tahtamouni ◽  
N. S. Najeeb ◽  
N. M. Issa ◽  
Z. A. Al-Mazaydeh ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Y Chromosome ◽  
Genomic Integrity ◽  
Sequence Tagged Sites ◽  
Chromosome Sequence

scholarly journals The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fish

BMC Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Lisui Bao ◽  
Changxu Tian ◽  
Shikai Liu ◽  
Yu Zhang ◽  
Ahmed Elaswad ◽  
...  
Keyword(s):  
Sex Determination ◽  
Y Chromosome ◽  
Channel Catfish ◽  
Teleost Fish ◽  
Chromosome Sequence ◽  
Sex Determination Mechanisms

Y chromosome sequence variation and the history of human populations

10.1038/81685 ◽  
2000 ◽  
Vol 26 (3) ◽  
pp. 358-361 ◽  
Author(s):  
Peter A. Underhill ◽  
Peidong Shen ◽  
Alice A. Lin ◽  
Li Jin ◽  
Giuseppe Passarino ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Sequence Variation ◽  
Human Populations ◽  
Chromosome Sequence ◽  
History Of

Triplex-hybridizing bioconjugated gold nanoparticles for specific Y-chromosome sequence targeting of bull spermatozoa

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 2020-2028 ◽  
Author(s):  
L. Gamrad ◽  
R. Mancini ◽  
D. Werner ◽  
D. Tiedemann ◽  
U. Taylor ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Y Chromosome ◽  
Genomic Dna ◽  
Chromosome Sequence ◽  
Triplex Forming Oligonucleotide

Gold nanoparticle (AuNP)-triplex forming oligonucleotide (TFO) conjugates hybridize with genomic DNA and sperm heads.

Sign in / Sign up

Export Citation Format

Share Document