Pheno- and genotypic characterization and identification of novel subtypes of Peste des Petits Ruminants virus in domestic and captive wild goats in Northern Iraq

Background Peste des Petits Ruminants (PPR) is an acute or peracute contagious transboundary viral disease that mainly affects caprine and ovine and causes significant economic impact in developing countries. After two PPR virus outbreaks in 2011 and 2014, an investigation, from August 2015 to September 2016, was carried out in Northern Iraq when an increased morbidity and mortality rates were reported in the domestic and captive wild goats. In the present study, ten domestic goat farms and seven captive wild goat herds located in seven geographical areas of Northern Iraq were clinically, pathologically, serologically and genotypically characterized to determine the prevalence and potential cause of PPR virus outbreak. Results The outbreak occurred with rate of morbidity (26.1%) and mortality (11.1%) in domestic goat farms as compared to captive wild goat herds where relatively high mortality (42.9%) and low morbidity (10.9%) rates were recorded. Based on the clinical symptoms (mucopurulent nasal discharges, ulceration and erosion of oral mucosa, profuse watery diarrhea) and necropsy (hemorrhage and congestion on mucous membranes of the colon and rectum with zebra stripes lesions) results, overall, the serological test findings revealed a high frequency (47.9%) of positive samples for anti-PPRV nucleoprotein antibodies. Furthermore, the nucleoprotein (N) gene was detected in 63.2 and 89.1% of samples using conventional and reverse transcription real-time quantitative PCR assays. A phylogenetic analysis of N gene amino acid sequences clustered with the reference strain revealed lineage IV similar to the strains isolated in 2011 and 2014, respectively. However, two sub-types of lineage IV (I and II), significantly distinct from the previous strains, were also observed. Conclusion The phylogenetic analysis suggests that movements of goats are possible cause and one of the important factors responsible for the spread of virus across the region. The study results would help in improving farm management practices by establishing a PPR virus eradication program using regular monitoring and vaccination program to control and mitigate the risk of re-emergence of PPR virus infection in domestic and captive wild goats in Iraq.

The Welfare of Domestic Goats (Capra hircus) in a Zoo-Based Animal-Visitor Interaction Program

We investigated whether the welfare of domestic goats in an animal-visitor interaction experience at a zoo was affected by the presence and behavior of visitors. We considered how the number of visitors in the goats’ habitat and visitor proximity to the goats impacted goat behavior. We also considered the goats’ behavior toward visitors, use of retreat space, and whether the visitors’ interaction style, specifically, how the visitors groomed the goats, was predictive of changes in the goats’ behavior. We conducted 29 hr of focal follows on 7 domestic goats (Capra hircus) at Lincoln Park Zoo, and analyzed data using mixed effects models. We found that goats preferred to be in the visitor yard as visitor numbers increased, and goat behaviors did not change as visitor numbers increased to the maximum allowed (16 people). There were no differences in rates of conspecific aggression or affiliation based on visitor presence or visitor interactions. However, there was a decrease in feeding and increase in self-maintenance behaviors specifically while visitors were in close proximity and interacting with goats. Furthermore, visitor-directed behavior, specifically head tossing, was more common when visitors groomed goats in an improper way. Overall, this study demonstrates that domestic goat welfare was relatively unaffected by the simple presence of visitors when maximum visitor limits were in place and retreat options were available, but that goats were sensitive to visitor interaction styles. These findings support the idea that domestic goats may be appropriate candidates for human-visitor interaction programs under specific conditions.

THE COCCIDIAN SPECIES PARASITIZING THE DOMESTIC GOAT IN NIGERIA

Nine fully recognized and described species of coccidia in goats and also one species not previously described are identified. Size shape, colour, sporulation time, morphology of unsporulated oocysts were the criteria for species identification 30 out of 36 faecal sample were positive form single or mixed infections with 3 samples being pure infections of single species. The unidentified species has a low occurrence and probably is not of importance in clinical coccidiosis in goats. The sporulation time for different species is found to be directly related to the size of the oocysts. As most species sporulate within 48 hours, daily removal of faecal materials form goat pens is recommended

DEVELOPMENT OF A SYSTEM FOR MONITORING AND PRESERVATION OF GENETIC RESOURCES ON THE EXAMPLE OF ABORIGENOUS GOATS OF THE ALTAI MOUNTAINS OF MONGOLIA

The origin of genetic resources domesticated by a species of animals, their importance in the development of fundamental biology and economics is considered on the example of a domestic goat. The data on genetic risks and population structure of local goat breeds inhabiting the territory of Mongolian Altai, obtained by analyzing SNP genotypes, microsatellite sequences, as well as the mitochondrial genome and genes of the Y chromosome, are presented.