Behavioural Impact of a Calming Herbal Supplement Fed to Hounds

The growing pet food market for feed supplements offering behavioural support has led to an increase in popularity of use, often without sufficient evidence to support claims. In particular, it is questionable whether various “calming” herbs and herbal blends are truly providing the desired outcomes. This study investigated whether a calming herbal nutraceutical for dogs had an impact on canine behaviour and to what effect. Thirty male English Foxhounds, Canis lupus familiaris, were fed the nutraceutical daily for four weeks following a control period. Overnight behavioural observations from video footage were carried out using scan sampling at sixty second intervals for six-hour periods daily, both in the control week and week four of the feeding trial. Stranger approach tests were also performed during these weeks on three separate occasions. Observation data were analysed using the paired t-test or Wilcoxon signed rank test. The paired t-test indicated a significant reduction in agonistic interactions, play, autogrooming, locomotion and stretching within the group. The stranger approach tests showed a significant increase in the number of hounds approaching the stranger, moving away, lying, standing, and sitting during the trial week, and a significant decrease in instances of hounds resting. Reduction in group agonistic and play interactions overnight suggests signs of a “calmer” pack. Increase in rest overnight may have impacted daytime behaviours, specifically in response to the stranger approach test. The results warrant further investigation of calming herbal nutraceuticals and their potential value as a tool in a holistic approach to managing canine behaviour.

Comparative anatomy of selected bones of forelimb of local Mongrelian Dog (Canis lupus familiaris) in Sokoto, Nigeria

This research was conducted over period of 3 months with the aim of studying Age related changes of selected bones of forelimb (Scapula, Humerus, Radius and Ulna) in Local Mongrelian Dog (Canis lupus familiaris). The study entails biometrical and gross observations on the bones. The sample bones were acquired from the experimental animals of comparative anatomy in the department. The bones were categorized into various age groups for the research. The length, width, diameter and circumference of the samples (scapula, humerus, radius and ulna bones) were determined for all the groups. The shape, size, color, location, position and relation of each segment of the samples at various stages of development were determined. The differences across the age groups of different samples were observed and recorded. Based on the research result, it was concluded that, the biometric and morphometry data was found to be increasing with advancement of age. A baseline data was established with the view to enhance learning.

Characterization of Triatomine Bloodmeal Sources Using Direct Sanger Sequencing and Amplicon Deep Sequencing Methods

Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely. We used two methods—Sanger sequencing and amplicon deep sequencing—to target a 228 bp region of the vertebrate CYTB gene and determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct sanger sequencing of PCR amplicons was successful for 36 samples (31%). Sanger sequencing revealed 15 distinct host species, which included humans, domestic animals (Canis lupus familiaris, Ovis aries, Gallus gallus, Bos taurus, Felis catus, and Capra hircus), wildlife (Rattus rattus, Bufo nebulifer, Sciurus carolinensis, Sciurus niger, Odocoileus virginianus), and captive animals (Panthera tigris, Colobus spp., Chelonoidis carbonarius). Samples sequenced by the Sanger method were also subjected to Illumina MiSeq amplicon deep sequencing. The amplicon deep sequencing results (average of 302,080 usable reads per sample) replicated the host community revealed using Sanger sequencing, and detected additional hosts in five triatomines (13.9%), including two additional blood sources (Procyon lotor, Bassariscus astutus). Up to four bloodmeal sources were detected in a single triatomine (Bufo nebulifer, Homo sapiens, Canis lupus familiaris, and Sciurus carolinensis). Enhanced understanding of vector-host-parasite networks may allow for integrated vector management programs focusing on highly-utilized and highly-infected host species.

QimmeqHealth—thyroid status of Greenland sled dogs (Canis lupus familiaris borealis)

Background Greenland sled dogs (GSD) are a unique, genetically isolated population of dogs living under exceptional environmental conditions. Metabolism, and thereby thyroid hormones are affected by multiple factors. Among other activity, energy balance and environmental conditions are important. A breed-specific reference interval (RI) can be useful for diagnostics of potential thyroid-related pathologies. The aim of this study was to establish RIs of the thyroid hormones thyroxin (T4), free thyroxin (fT4), and thyroid stimulating hormone (TSH) in GSD. In addition to evaluate the effect of sex, age, season, management, and body condition score (BCS) in GSD. Physical exams and cephalic venous blood sampling were performed in the period of 2018–2019 from 265 GSD managed either privately or by the Danish navy. Serum biochemical analyses, including C-reactive protein, were performed and RIs were determined for TSH, T4 and fT4 in only healthy dogs. The RIs were determined using American Society for Veterinary Clinical Pathology guidelines and the effect of varying factors were evaluated by linear regression and further tested by Mann–Whitney test. Results 144 GSD were included in the reference group resulting in RIs: T4: 6.44–48.65 nmol/L; fT4: 3.91–18.51 pmol/L; and TSH: 0.04–0.55 ng/mL. Female GSD had significantly higher concentrations of T4 (P = 0.039) and fT4 (P = 0.015) compared to males; a positive correlation between TSH and aging was found; T4 concentrations were significantly higher (P = 0.003) during summer; and TSH concentrations were lower in GSD managed by the navy (P < 0.0001). BCS was higher (P < 0.0001) in Sirius GSD compared to civilian GSD, and BCS was positively correlated with T4 and negatively correlated with TSH. Conclusions Reference intervals for T4, fT4 and TSH in GSD were established. The RI for T4 and fT4 was lower compared to other breeds. In addition, sex, age, season, management and BCS demonstrated variable effects on thyroid hormones. Our results can be used as a foundation for improving management and further research of GSD.

Aerobic Isolates from Gestational and Non-Gestational Lactating Bitches (Canis lupus familiaris)

Mastitis is a complex and well-defined mammary gland pathology, and an emergency in bitches. In dogs, its prevalence is about 1% of all reported diseases and about 5.3% of all reproductive pathologies. Lactating bitches are naturally prone to developing mastitis since puppies can easily overstimulate the epidermal layer of nipples during feeding, facilitating bacterial colonization of the glands. This study aimed to describe the aerobic bacterial flora isolated from milk samples derived from a cohort of patients (n = 87) diagnosed with clinical mastitis (n = 29), subclinical mastitis (n = 17) and healthy mammary glands (n = 46). All of the patients underwent a gynecology consultation to diagnose mammary gland afflictions; physical examination results were coupled with traditional hematological findings. The milk samples were plated on specific microbiological media for bacterial isolation. Among the 162 milk samples analyzed, 93.2% (151/162) had a positive microbiological result, while 6.8% (11/162) were sterile. The bacteriological profile of the milk samples showed 47 different species. The most common bacterial families detected in healthy bitches and bitches with subclinical and clinical mastitis were the Staphylococcaceae, Enterobacteriaceae and Enterococcaceae families. The results indicated that half of the isolated bacteria are novel findings in dogs and that some of them are normal components of human milk.

Optimization of rabies (Rhabdoviridae: Lyssavirus) dog vaccination schedule using a mathematical model

Introduction. Most cases of human rabies are caused by dog (Canis lupus familiaris) bites. Therefore, the implementation of vaccination programs of these animals is one of the urgent tasks.The work aims to identify the factors influencing the production of antirabies virus-neutralizing antibodies (VNAs) in vaccinated dogs, and to formulate recommendations for adjusting the vaccination schedule using mathematical modeling (MM).Material and methods. We used a fixed-effects modeling procedure to estimate the two-compartment model parameters using log-transformed data (obtained by RFFIT, rapid fluorescent focus inhibition test; and FAVN, fluorescent antibody virus-neutralization test) on the VNAs levels in the serum of vaccinated dogs.Results. More vigorous immune response after a two-dose primary vaccination is formed in juvenile dogs at the age of 3 months to 1 year compared to the adult dogs. Following the primary vaccination and revaccination 1 year after, VNAs were produced more intensively in adult stray dogs than in domestic dogs.Discussion. The short-term immune response observed in dogs aged up to 3 months is due to the presence of colostral antibodies and the active growth of the organism at this age. The results of our study confirm that most of the dogs have a level of antirabies VNAs of ≥0.5 IU/ml up to two or more years following immunization. However, only regular annual revaccination ensures the protective VNAs level in animals that responded poorly to vaccination due to various factors.Conclusion. The following antirabies vaccination schedule is recommended: primary vaccination of the dog at the age of 3 months up to 1 year with 1–2 month intervals, then revaccination annually. This work also demonstrates the possibility of a wider application of MM methods for solving problems of vaccine prevention.

The case for case studies: A new approach to evaluating the effectiveness of livestock protection tools

Livestock operations in California face livestock losses due to a range of carnivore species. Simultaneously, there is an increased call to reduce the use of lethal predator control methods and replace them with nonlethal methods. Livestock guardian dogs (Canis lupus familiaris) are one such nonlethal livestock protection tool (LPT), yet research is still lacking on the factors and situations that impact their effectiveness. Using three case studies, we demonstrate the value of objective analyses that explicitly address the inherent differences in ranch management, environment, and surrounding land uses in examining livestock guardian dogs as an LPT. We used semi-structured questionnaire surveys of livestock operators to collect information on effectiveness, behavior, and producer satisfaction of LGDs protecting poultry (Gallus gallus domesticus), calves (Bovus taurus), and sheep (Ovis aries) on private and public land and in conjunction with a variety of other livestock protection tools. We aimed to address all aspects related to the use of LGDs as a means of informing livestock operators’ decisions on whether LGDs are an appropriate tool for a particular operation. The case studies demonstrated the complexities involved in applying LGDs as a LPT within the context of a livestock operation. In two of the three case studies, LGDs did not entirely eliminate livestock losses yet operator satisfaction remained high.

Domestic dogs (Canis lupus familiaris) are sensitive to the correlation between pitch and timbre in human speech

The perceived pitch of human voices is highly correlated with the fundamental frequency (f0) of the laryngeal source, which is determined largely by the length and mass of the vocal folds. The vocal folds are larger in adult males than in adult females, and men’s voices consequently have a lower pitch than women’s. The length of the supralaryngeal vocal tract (vocal-tract length; VTL) affects the resonant frequencies (formants) of speech which characterize the timbre of the voice. Men’s longer vocal tracts produce lower frequency, and less dispersed, formants than women’s shorter vocal tracts. Pitch and timbre combine to influence the perception of speaker characteristics such as size and age. Together, they can be used to categorize speaker sex with almost perfect accuracy. While it is known that domestic dogs can match a voice to a person of the same sex, there has been no investigation into whether dogs are sensitive to the correlation between pitch and timbre. We recorded a female voice giving three commands (‘Sit’, ‘Lay down’, ‘Come here’), and manipulated the recordings to lower the fundamental frequency (thus lowering pitch), increase simulated VTL (hence affecting timbre), or both (synthesized adult male voice). Dogs responded to the original adult female and synthesized adult male voices equivalently. Their tendency to obey the commands was, however, reduced when either pitch or timbre was manipulated alone. These results suggest that dogs are sensitive to both the pitch and timbre of human voices, and that they learn about the natural covariation of these perceptual attributes.