Electroactivated Disposable Pencil Graphite Electrode – New, Cost‐effective, and Sensitive Electrochemical Detection of Bioflavonoid Hesperidin

2020 ◽  
Author(s):  
Silvija Šafranko ◽  
Anamarija Stanković ◽  
Abdelilah Asserghine ◽  
Martina Jakovljević ◽  
Sugato Hajra ◽  
...  
2016 ◽  
Vol 8 (38) ◽  
pp. 6974-6981 ◽  
Author(s):  
Kathiresan Vijayaraj ◽  
Suck Won Hong ◽  
Sung-Ho Jin ◽  
Seung-Cheol Chang ◽  
Deog-Su Park

A disposable glucose biosensor has been fabricated on the surface of a cost-effective pencil graphite electrode (PGE) by an electrochemical method, using glucose oxidase (GOx) and reduced graphene oxide (rGO).


2020 ◽  
Vol 11 (2) ◽  
pp. 8702-8715

Salmonella enterica is considered one of the most common bacterial agent causes of acute gastroenteritis and foodborne illness in humans worldwide. Antibiotic-resistant is considered as a major problem in Salmonella enterica Serovar. This study introduces a new simple and sensitive aptasensor based on chitosan (Chi)-electrospun carbon nanofibers (CNF) /gold nanoparticles (GNPs) decorated pencil graphite electrode (GE) as a novel platform for electrochemical detection of Salmonella enterica Serovar. A Salmonella-specific recognition aptamer ssDNA sequence was used in the development of this voltammetric biosensor. Electrochemical behaviors of electrodes; unmodified GE, CNF-Chi/GE, GNPs/CNF-Chi/GE, GNPs/CNF-Chi/GEs linked with the aptamer were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). After the optimization of experimental conditions (e.g., CNF concentration, pH, and incubation time), electrochemical detection of Salmonella was performed via differential pulse voltammetry (DPV) in methylene blue solution. The designed aptasensor exhibited a linear range of 10 to 105 (CFU/mL) with the limit of detection (LOD) 1.223 (Cfu/mL) for Salmonella. This aptasensor displayed excellent selectivity and remarkable sensitivity in terms of the detection of Salmonella enterica even in the real samples as compared to the polymerase chain reaction (PCR) technique. The constructed aptasensor is a highly sensitive sensor for the detection of Salmonella enterica and also can be tailored for various other targets.


RSC Advances ◽  
2020 ◽  
Vol 10 (39) ◽  
pp. 22871-22880
Author(s):  
C. Kavitha ◽  
K. Bramhaiah ◽  
Neena S. John

A low cost reduced graphene oxide–copper hybrid nano thin-film modified pencil graphite electrode has been employed to detect the l-tyrosine enantiomer.


Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 351
Author(s):  
Iulia Gabriela David ◽  
Alexandra-Gabriela Oancea ◽  
Mihaela Buleandră ◽  
Dana Elena Popa ◽  
Emilia Elena Iorgulescu ◽  
...  

Diosmin (DIO) is a naturally occurring flavonoid with multiple beneficial effects on human health. The presence of different hydroxyl groups in diosmin structure enables its electrochemical investigation and quantification. This work presents, for the first time, diosmin voltammetric behavior and quantification on the cost-effective, disposable pencil graphite electrode (PGE). Diosmin oxidation on PGE involves two irreversible steps, generating products with reversible redox behaviors. All electrode processes are pH-dependent and predominantly adsorption-controlled. Differential pulse (DPV) and adsorptive stripping differential pulse (AdSDPV) voltammetric methods have been optimized for diosmin quantification o an H-type PGE, in 0.100 mol/L H2SO4. The linear ranges and limits of detection were for DPV 1.00 × 10−6–1.00 × 10−5 mol/L and 2.76 × 10−7 mol/L DIO for DPV and 1.00 × 10−7–2.50 × 10−6 mol/L and 7.42 × 10−8 mol/L DIO for AdSDPV, respectively. The DPV method was successfully applied for diosmin quantification in dietary supplement tablets. The percentage recovery was 99.87 ± 4.88%.


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