A user-friendly alternative to formaldehyde-based DNA silver-staining method on polyacrylamide gels

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.

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Combined beta-galactosidase and immunogold/silver staining for immunohistochemistry and DNA in situ hybridization.

Journal of Histochemistry & Cytochemistry ◽

10.1177/38.3.1689335 ◽

1990 ◽

Vol 38 (3) ◽

pp. 325-329 ◽

Cited By ~ 19

Author(s):

W van den Brink ◽

C van der Loos ◽

H Volkers ◽

R Lauwen ◽

F van den Berg ◽

...

Keyword(s):

In Situ Hybridization ◽

Silver Staining ◽

Staining Method ◽

Double Staining ◽

Reaction Products ◽

Silver Enhancement ◽

Silver Staining Method ◽

Double Staining Method ◽

Beta Galactosidase

A combination of beta-galactosidase enzyme and the immunogold/silver staining method was studied for evaluation of double-staining experiments. Applications are shown for immunohistochemical double staining using two monoclonal antibodies and for combined immunohistochemistry and DNA in situ hybridization in one tissue section. The following advantages for the present double-staining method were evaluated: superior sensitivity of the immunogold/silver staining method for at least one epitope, which also allows detection of biotinylated DNA probes. The structure of the indolyl precipitate after revelation of beta-galactosidase activity did not show a concealing effect during a sequential double-staining method, as compared with the visualization of peroxidase with diaminobenzidine. These factors, and the sharply contrasting colored reaction products of beta-galactosidase (blue-green) and the immunogold/silver staining method including silver enhancement (brown-black), allow clear distinction of mixed-stained cell constituents.

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A silver staining method for protein bands or immunoprecipitates in electrophoresis or immunoelectrophoretic treatment of unconcentrated cerebrospinal fluid proteins using the commercial agarose plates (Corning Universal System)

Journal of Neuroscience Methods ◽

10.1016/0165-0270(82)90072-3 ◽

1982 ◽

Vol 5 (3) ◽

pp. 221-226 ◽

Cited By ~ 3

Author(s):

Atsushi Hiraoka ◽

Isao Miura ◽

Osamu Murao ◽

Nobuo Kawahara ◽

Itaru Tominaga ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Silver Staining ◽

Staining Method ◽

Universal System ◽

Cerebrospinal Fluid Proteins ◽

Silver Staining Method

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Changes in the number and size of nucleoli of Chara vulgaris L. antheridial filament cells during the period preceding light-induced re-initiation of cell divisions following a mitodepressive effect of darkness

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1999.033 ◽

2014 ◽

Vol 68 (4) ◽

pp. 251-254

Author(s):

Maria Kwiatkowska ◽

Ewa Papiernik

Keyword(s):

Cell Cycle ◽

Developmental Stage ◽

Mitotic Activity ◽

Silver Staining ◽

Staining Method ◽

Chara Vulgaris ◽

Cell Divisions ◽

A Cell ◽

Silver Staining Method

The changes in number and size of nucleoli of <em>Chara vulgaris</em> antheridial filament cells were monitored with the use of Howell and Black's silver staining method. After a 3-day mitodepressive treatment with darkness the cells were exposed to light which reactivated mitotic activity after 18-20 hours. Eight-celled antheridial filaments were observed. In the period preceding light-induced re-initiation of mitoses a gradual reconstruction of the number and size of nucleoli characteristic of control, as well as their total area per nucleus appeared. The obtained results indicate that one of the important conditions for a cell to be able to divide is accumulation of nucleolus components characteristic of a given developmental stage and this controls nucleologenesis of the subsequent cell cycle.

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