Application of capillary electrophoresis technique for the enantioseparation of bioactive ferrocene‐based compounds versus DFT calculated data

In the present study, two analytical methods for the residue analysis of oxytetracycline in milk sample have been generated. In HPLC method, the analysis was performed on an X Terra RP-18 column at 25 °C with the mobile phase as methanol: water (20 : 80 )(v/v) modified to pH 5. For the second method capillary electrophoresis system was used. The analysis of oxytetracycline in milk sample could be achieved without using organic modifier in a 58 cm length capillary at a working voltage of 12 kV with 20 mM NaH2PO4-H3PO4 (pH 7) by capillary electrophoresis. Tetracycline was used as internal standard in both methods. The results calculated from both methods were compared to each other. The calculated data for drugs was checked with the data predicted by the SPARC on-line pKa estimator.

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A Capillary Electrophoresis Technique for Evaluating Botulinum Neurotoxin B Light Chain Activity

Journal of Protein Chemistry ◽

10.1023/b:jopc.0000005459.00492.60 ◽

2003 ◽

Vol 22 (5) ◽

pp. 441-448 ◽

Cited By ~ 9

Author(s):

Michael Adler ◽

Harlan F. Shafer ◽

Heather A. Manley ◽

Brennie E. Hackley, Jr. ◽

James D. Nicholson ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Light Chain ◽

Botulinum Neurotoxin ◽

Botulinum Neurotoxin B ◽

Electrophoresis Technique

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Effectiveness of Capillary Electrophoresis Fluoroimniunoassay of Blood PrPSc for Evaluation of Scrapie Pathogenesis in Sheep

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870701900516 ◽

2007 ◽

Vol 19 (5) ◽

pp. 552-557 ◽

Cited By ~ 5

Author(s):

David J. Everest ◽

Susan Waterhouse ◽

Tina Kelly ◽

Elena Velo-Rego ◽

Maurice J. Sauer

Keyword(s):

Capillary Electrophoresis ◽

Prion Protein ◽

Blood Test ◽

Diagnostic Performance ◽

Prion Diseases ◽

Present Form ◽

Similar Proportion ◽

Specificity And Sensitivity ◽

Test Specificity ◽

Electrophoresis Technique

Management of prion diseases in livestock would benefit greatly from availability of a validated blood test. A promising immunocapillary electrophoresis technique (also known as capillary electrophoresis fluoroimmunoassay) to detect abnormal prion protein in blood from live sheep is evaluated here. Capillary electrophoresis fluoroimmunoassay was applied to analysis of extracted blood from scrapie-exposed sheep ( n = 87; 347 samples) at various stages of incubation, and to control sheep ( n = 194; 489 samples). Overall, test values for the control and test populations were not significantly different, and a similar proportion of control (7%) and test (10%) sheep were classified as positive. Over 2−3 month intervals from birth until clinical disease, test specificity and sensitivity ranged from 66.7% to 100% and 0% to 66.7%, respectively, indicating poor diagnostic performance at all stages of pathogenesis. In routine application, in its present form, the capillary electrophoresis fluoroimmunoassay procedure proved to be insufficiently robust for use as a blood test for scrapie diagnosis.

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