Novel Capillary Electrophoresis Technique for the Study of Plant Phenolic Enzymic Oxidation Mechanisms

Management of prion diseases in livestock would benefit greatly from availability of a validated blood test. A promising immunocapillary electrophoresis technique (also known as capillary electrophoresis fluoroimmunoassay) to detect abnormal prion protein in blood from live sheep is evaluated here. Capillary electrophoresis fluoroimmunoassay was applied to analysis of extracted blood from scrapie-exposed sheep ( n = 87; 347 samples) at various stages of incubation, and to control sheep ( n = 194; 489 samples). Overall, test values for the control and test populations were not significantly different, and a similar proportion of control (7%) and test (10%) sheep were classified as positive. Over 2−3 month intervals from birth until clinical disease, test specificity and sensitivity ranged from 66.7% to 100% and 0% to 66.7%, respectively, indicating poor diagnostic performance at all stages of pathogenesis. In routine application, in its present form, the capillary electrophoresis fluoroimmunoassay procedure proved to be insufficiently robust for use as a blood test for scrapie diagnosis.

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Ionic liquids and cyclodextrin inclusion complexes: limitation of the affinity capillary electrophoresis technique

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-016-9931-z ◽

2016 ◽

Vol 408 (28) ◽

pp. 8211-8220 ◽

Cited By ~ 6

Author(s):

Nadine Mofaddel ◽

Sophie Fourmentin ◽

Fréderic Guillen ◽

David Landy ◽

Géraldine Gouhier

Keyword(s):

Capillary Electrophoresis ◽

Ionic Liquids ◽

Inclusion Complexes ◽

Affinity Capillary Electrophoresis ◽

Cyclodextrin Inclusion Complexes ◽

Cyclodextrin Inclusion ◽

Electrophoresis Technique

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False measurement of glycated hemoglobin in patients without hemoglobin A

Bioscience Reports ◽

10.1042/bsr20180128 ◽

2019 ◽

Vol 39 (1) ◽

Cited By ~ 3

Author(s):

Minghuan Suo ◽

Dongmei Wen ◽

Weijia Wang ◽

Decai Zhang ◽

Shengnan Xu ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Glycated Hemoglobin ◽

Biochemical Marker ◽

Compound Heterozygous ◽

Test Results ◽

Ion Exchange Hplc ◽

Hemoglobin Variants ◽

Hba1c Measurement ◽

Compound Heterozygotes ◽

Electrophoresis Technique

Abstract Background: Hemoglobin (Hb) A1c, a biochemical marker widely used in monitoring diabetes mellitus, can be quantitatively measured by various examining systems. However, significant errors still exist. In the present study, we evaluated the HbA1c level in five patients with compound heterozygotes by five different examining systems and our goal is to identify the existence of erroneous HbA1c measurement. Methods: Blood samples collected from normal (no hemoglobin variants) and abnormal (compound heterozygotes) patients were analyzed by capillary electrophoresis technique and sequence analysis. The samples without HbA expression via above methods were further analyzed for HbA1c by ion exchange HPLC Variant II/ Variant II Turbo 2.0 (VII and VII-T 2.0), boronate affinity HPLC, capillary electrophoresis, and Tinaquant immunoassay. Results: HbA1c expression were unexpectedly detected in the compound heterozygous samples by using additional examining systems: The HPLC VII and VII-T 2.0 detected HbA1c expression in two of five samples and failed to detect the abnormal HbA2 expression; the CE system detected HbA1c expression in one of five samples with abnormal HbA2 expression; the Ultra2 and PPI system detected the HbA1c expression of all samples without abnormal HbA2. Conclusions: Five human samples without HbA expression were additionally detected with HbA1c expression with or without abnormal HbA2 expression by five analysis systems and the different examining assay potentially affected the test results. These results demonstrated that the limitations of current examining systems for monitoring patients with hemoglobin disorders highlighting the further improvement in the method of clinical HbA examination.

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