Phenotypic characterization of neural stem cells from human fetal spinal cord: Synergistic effect of LIF and BMP4 to generate astrocytes

Glia ◽  
2007 ◽  
Vol 55 (11) ◽  
pp. 1156-1168 ◽  
Author(s):  
Michael W. Weible ◽  
Tailoi Chan-Ling
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Synergistic Effect ◽  
Neural Stem Cells ◽  
Phenotypic Characterization

scholarly journals Synergistic Effect of Neural Stem Cells and Olfactory Ensheathing Cells on Repair of Adult Rat Spinal Cord Injury

2010 ◽  
Vol 19 (10) ◽  
pp. 1325-1337 ◽  
Author(s):  
Gan Wang ◽  
Qiang Ao ◽  
Kai Gong ◽  
Huancong Zuo ◽  
Yandao Gong ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Synergistic Effect ◽  
Neural Stem Cells ◽  
Olfactory Ensheathing Cells ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Cord Injury ◽  
Adult Rat Spinal Cord

Isolation and characterization of neural stem cells from fetal canine spinal cord

2021 ◽  
Vol 765 ◽  
pp. 136293
Author(s):  
Sarah Ingrid Pinto Santos ◽  
Vanessa Cristina de Oliveira ◽  
Naira Caroline Godoy Pieri ◽  
Fabiana Fernandes Bressan ◽  
Carlos Eduardo Ambrósio ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Isolation And Characterization

Characterization of neural stem cells modified with hypoxia/neuron-specific VEGF expression system for spinal cord injury

Gene Therapy ◽  
2017 ◽  
Vol 25 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Y Yun ◽  
J Oh ◽  
Y Kim ◽  
G Kim ◽  
M Lee ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Expression System ◽  
Vegf Expression ◽  
Cord Injury

scholarly journals Immunosuppressants Affect Human Neural Stem Cells In Vitro but Not in an In Vivo Model of Spinal Cord Injury

2013 ◽  
Vol 2 (10) ◽  
pp. 731-744 ◽  
Author(s):  
Christopher J. Sontag ◽  
Hal X. Nguyen ◽  
Noriko Kamei ◽  
Nobuko Uchida ◽  
Aileen J. Anderson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Model ◽  
Human Neural Stem Cells ◽  
Cord Injury

Targeted Treatment of Experimental Spinal Cord Glioma With Dual Gene-Engineered Human Neural Stem Cells

Neurosurgery ◽  
2015 ◽  
Vol 79 (3) ◽  
pp. 481-491 ◽  
Author(s):  
Alexander E. Ropper ◽  
Xiang Zeng ◽  
Hariprakash Haragopal ◽  
Jamie E. Anderson ◽  
Zaid Aljuboori ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Growth Rate ◽  
Neural Stem Cells ◽  
Tumor Growth ◽  
Rat Model ◽  
Weight Bearing ◽  
Tumor Growth Rate ◽  
Intramedullary Spinal Cord ◽  
Human Neural Stem Cells

Abstract BACKGROUND There are currently no satisfactory treatments or experimental models showing autonomic dysfunction for intramedullary spinal cord gliomas (ISCG). OBJECTIVE To develop a rat model of ISCG and investigate whether genetically engineered human neural stem cells (F3.hNSCs) could be developed into effective therapies for ISCG. METHODS Immunodeficient/Rowett Nude rats received C6 implantation of G55 human glioblastoma cells (10K/each). F3.hNSCs engineered to express either cytosine deaminase gene only (i.e., F3.CD) or dual genes of CD and thymidine kinase (i.e., F3.CD-TK) converted benign 5-fluorocytosine and ganciclovir into oncolytic 5-fluorouracil and ganciclovir-triphosphate, respectively. ISCG rats received injection of F3.CD-TK, F3.CD, or F3.CD-TK debris near the tumor epicenter 7 days after G55 seeding, followed with 5-FC (500 mg/kg/5 mL) and ganciclovir administrations (25 mg/kg/1 mL/day × 5/each repeat, intraperitoneal injection). Per humane standards for animals, loss of weight-bearing stepping in the hindlimb was used to determine post-tumor survival. Also evaluated were autonomic functions and tumor growth rate in vivo. RESULTS ISCG rats with F3.CD-TK treatment survived significantly longer (37.5 ± 4.78 days) than those receiving F3.CD (21.5 ± 1.75 days) or F3.CD-TK debris (19.3 ± 0.85 days; n = 4/group; P <.05, median rank test), with significantly improved autonomic function and reduced tumor growth rate. F3.DC-TK cells migrated diffusively into ISCG clusters to mediate oncolytic effect. CONCLUSION Dual gene-engineered human neural stem cell regimen markedly prolonged survival in a rat model that emulates somatomotor and autonomic dysfunctions of human cervical ISCG. F3.CD-TK may provide a novel approach to treating clinical ISCG.

Engraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injury

Cytotherapy ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 313-325 ◽  
Author(s):  
Siobhan S. McMahon ◽  
Silke Albermann ◽  
Gemma E. Rooney ◽  
Georgina Shaw ◽  
Yolanda Garcia ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Contusion Injury

(285) Spinal cord-derived neural stem cells restore hyperalgesic responses previously diminished by chemical ablation of NK-1 expressing neurons in the rat spinal cord

2015 ◽  
Vol 16 (4) ◽  
pp. S47
Author(s):  
G. Strichartz ◽  
J. Wang ◽  
S. Khasabov ◽  
K. Kojima ◽  
D. Simone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Chemical Ablation
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