Development and novel design of clustery Graphene Oxide formed Conductive Silk hydrogel cell vesicle to Repair Myocardial Infarction: Investigation of its biological activity for cell delivery applications

Cell delivery therapy is a talented and hopeful strategic approach to repair the damaged cardiac tissues after myocardial infarction. The biocompatible injectable hydrogel with bioactive nanomaterials with effective self-healing capability has been highly required for the cell delivery and regeneration therapy. In the present study, we have developed the biopolymeric and biocompatible self-healing silk protein hydrogel matrix with graphene oxide nanoformulations as cell delivery vehicles for the treatment of myocardial infarction regeneration. The materials combinations, structural interactions, thermal stability and morphology of the designed hydrogel were investigated and elaborated. To improve therapeutic potential of the hydrogel matrix, growth factor was loaded and investigated its biological activity such as cell survival and cell proliferations properties by using endothelial progenitor cells. Collected, these developed materials are excellent vesicle for cell therapy in myocardial infarction.

Recombinant Silk Hydrogel as a Novel Dermal Filler Component: Preclinical Safety and Efficacy Studies of a New Class of Tissue Fillers

Background Hyaluronic acid-based tissue fillers are commonly utilized in reconstructive surgery as well as for aesthetic augmentation. A new type of recombinant silk-based tissue filler might pose a beneficial alternative for surgeons and patients. Objectives The aim of this study was to compare injectability, reshaping, tolerability, and postimplantation behavior of dermal filler preparations containing recombinant silk hydrogel with a commercially available hyaluronic acid filler in 2 different animal models. Methods Recombinant silk hydrogel as standalone preparation or as a mixture with commercial stabilized hyaluronic acid was tested in rodent and porcine animal models. The preparations were analyzed in detail and administered subdermally followed by clinical, volumetric, and histological monitoring of the subdermal depots over several months. Results Applicability, dosing, and tissue distribution of the filler preparations were facilitated in the presence of silk hydrogel. No clinical complications attributable to tissue filler application were recorded. State-of-the art methods, such as high-performance magnetic resonance imaging, were applied successfully to monitor the volumetric development of the filler depots in live animals. Conclusions The preclinical data demonstrate the basic suitability of recombinant silk hydrogel as safe and convenient tissue filler ingredient. Due to its shear thinning properties, recombinant silk hydrogel has the potential for less painful application, comfortable aesthetic reshaping immediately after administration, and negligible postoperative discomfort.

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

A novel methodology for the development of cell-laden silk hydrogels using a biocompatible photo-initiator.

A Composited Povidone-Iodine Silk Fibroin Hydrogel for Wound Infection

Wound infections have historically been an important global health concern which seriously affects human life and health. Reducing the number of bacteria at the site of the wound is essential to prevent wound infection and promote wound healing. Povidone-iodine (PVP-I) is widely used as an antiseptic providing useful properties for local anti-infective treatment in skin, mucous membranes, and wounds. In this study, we synthesized a new PVP-I/silk fibroin (SF) hydrogel through a simple preparation method, and to study the physicochemical properties, antibacterial properties and biocompatibility of the composited hydrogel. Compared with the pure SF hydrogel, the composition of PVP-I with SF hydrogels endowed the hydrogel new physicochemical characteristics especially enhanced hydrogel's structural stability and a sustained-release effect of iodine. Moreover, such composited hydrogels showed better antibacterial properties when the content of the compound PVP-I reached a certain degree. In vivo, the results indicate that the composited hydrogel displayed a good histocompatibility and biodegradability. All these results demonstrated that the composited povidone-iodine silk hydrogel can be fabricated as an anti-infective biomaterials with great potential using in wound infection.