Establishment and characterization of a new human B-cell line (ONHL-1) from non-Hodgkin's lymphoma: Constant expression ofbcl-2 gene during mitogen-induced growth inhibition

Abstract A unique B-cell non-Hodgkin's lymphoma (NHL) cell line (Karpas 422), bearing both t(14;18) and t(4;11) chromosomal translocations as well as several other chromosomal abnormalities, has been established from the pleural effusion of a patient with chemotherapy-resistant NHL. This cell line has the same karyotypic features as malignant cells from the patient. The major cell clone is characterized chromosomally by 46,XX t(2;10)(p23;q22.1), t(4;11)(q21.3; q23.1), t(14;18)(q32.1;q21.3), t(4;16)(q21.3;p13.1). Both phenotypically and genotypically, the cell line has features of a mature B-cell neoplasm with no evidence for commitment to other lineages. Rearrangements of the C-ETS-1 oncogene and N-CAM-1 and CD3 genes that map to 11q23 were not detected by conventional Southern analysis. BCL-2 was rearranged within the major breakpoint cluster. The K422 cell line has a unique karyotype; this is the first occasion that the t(4;11) translocation has been described in a t(14;18) lymphoma. The cell line will be of value in determining the molecular nature of the t(4;11) translocation.

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A new human B-cell non-Hodgkin's lymphoma cell line (Karpas 422) exhibiting both t (14;18) and t(4;11) chromosomal translocations

Blood ◽

10.1182/blood.v75.3.709.bloodjournal753709 ◽

1990 ◽

Vol 75 (3) ◽

pp. 709-714 ◽

Cited By ~ 1

Author(s):

MJ Dyer ◽

P Fischer ◽

E Nacheva ◽

W Labastide ◽

A Karpas

Keyword(s):

B Cell ◽

Cell Line ◽

Hodgkin’S Lymphoma ◽

Chromosomal Abnormalities ◽

Cell Clone ◽

Hodgkin's Lymphoma ◽

Chromosomal Translocations ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma ◽

Human B Cell

A unique B-cell non-Hodgkin's lymphoma (NHL) cell line (Karpas 422), bearing both t(14;18) and t(4;11) chromosomal translocations as well as several other chromosomal abnormalities, has been established from the pleural effusion of a patient with chemotherapy-resistant NHL. This cell line has the same karyotypic features as malignant cells from the patient. The major cell clone is characterized chromosomally by 46,XX t(2;10)(p23;q22.1), t(4;11)(q21.3; q23.1), t(14;18)(q32.1;q21.3), t(4;16)(q21.3;p13.1). Both phenotypically and genotypically, the cell line has features of a mature B-cell neoplasm with no evidence for commitment to other lineages. Rearrangements of the C-ETS-1 oncogene and N-CAM-1 and CD3 genes that map to 11q23 were not detected by conventional Southern analysis. BCL-2 was rearranged within the major breakpoint cluster. The K422 cell line has a unique karyotype; this is the first occasion that the t(4;11) translocation has been described in a t(14;18) lymphoma. The cell line will be of value in determining the molecular nature of the t(4;11) translocation.

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Expression of the PAX5 /BSAP transcription factor in haematological tumour cells and further molecular characterization of the t(9;14)(p13;q32) translocation in B-cell non-Hodgkin's lymphoma

British Journal of Haematology ◽

10.1046/j.1365-2141.1998.00842.x ◽

1998 ◽

Vol 102 (3) ◽

pp. 691-700 ◽

Cited By ~ 24

Author(s):

Hamada ◽

Yonetani ◽

Ueda ◽

Maesako ◽

Akasaka ◽

...

Keyword(s):

Transcription Factor ◽

B Cell ◽

Molecular Characterization ◽

Hodgkin’S Lymphoma ◽

Tumour Cells ◽

Hodgkin's Lymphoma ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma

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Resistance to CD95-mediated apoptosis through constitutive c-FLIP expression in a non-Hodgkin's lymphoma B cell line

Leukemia ◽

10.1038/sj.leu.2401954 ◽

2000 ◽

Vol 14 (12) ◽

pp. 2149-2158 ◽

Cited By ~ 36

Author(s):

M Irisarri ◽

J Plumas ◽

T Bonnefoix ◽

M-C Jacob ◽

C Roucard ◽

...

Keyword(s):

B Cell ◽

Cell Line ◽

Hodgkin’S Lymphoma ◽

Hodgkin's Lymphoma ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma

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B-cell non-Hodgkin's lymphoma cell line (Karpas 1106) with complex translocation involving 18q21.3 but lacking BCL2 rearrangement and expression

Blood ◽

10.1182/blood.v84.10.3422.3422 ◽

1994 ◽

Vol 84 (10) ◽

pp. 3422-3428 ◽

Cited By ~ 29

Author(s):

E Nacheva ◽

MJ Dyer ◽

C Metivier ◽

D Jadayel ◽

G Stranks ◽

...

Keyword(s):

B Cell ◽

Cell Line ◽

Hodgkin’S Lymphoma ◽

Hodgkin's Lymphoma ◽

Derivative Chromosome ◽

Hindiii Site ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma ◽

Bcl2 Protein ◽

Conventional Cytogenetics

Abstract A B-cell non-Hodgkin's lymphoma (B-NHL) cell line (Karpas 1106) with an unusual three-way translocation involving 18q21.3 has been derived from a patient with mediastinal lymphoblastic B-NHL. Although conventional cytogenetics showed a derivative 18q-identical to that seen in cases with t(14;18)(q32.3;q21.3), no translocations of either chromosome 14 could be detected. Instead fluorescent in situ hybridization analysis using a chromosome-18 paint showed that the segment 18q21.3–18qter had become sandwiched on a derivative chromosome X between segments Xqter-c- Xq28 and 13q12-qter, with the centrometric site of 18q21.3 subband juxtaposed to the X sequences. Pulsed-field DNA blots failed to detect rearrangement of the BCL2 gene. Conventional DNA blots using a variety of restriction digests and both 52 and 32 BCL2 and FVT 1 probes also failed to detect rearrangement in Karpas 1106. A rearranged fragment seen only in HindIII digests with 52 BLC2 probes may represent a local microalteration, which is either a mutation or small deletion involving the HindIII site as seen in other cases of B-NHL. Neither BCL2 RNA nor BCL2 protein expression were detected. These and other data suggest that genes at 18q21.3, other than BCL2 and FVT1, may be targets for translocation in certain subgroups of B-NHL.

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