The effects of the treatment of tumor cells of MCa mammary carcinoma and TLX5 lymphoma with
the ruthenium complex Na[trans-RuCl4(DMSO)lm] for several transplant generations were studied on
tumor growth and metastases formation. On TLX5 lymphoma cells, treatment was performed in vitro
prior to in vivo inoculation of tumor cells in intact or immunesuppressed mice. Either considering tumor
take and growth or its capacity to invade the brain of the inoculated hosts, Na[trans-RuCl4(DMSO)lm] did not induce any significant modification. Conversely, in mice with MCa mammary carcinoma, the
in
vivo treatment of tumor cells in immunesuppressed hosts caused a progressive increase of DNA activity
and, starting from the 4th transplant generation, a significantly increased susceptibility of lung
metastasis formation to a further treatment in intact mice. These data seem to suggest that Na[trans-RuCl4(DMSO)Im] does not induce chemical xenogenization of tumor cells nor its repeated treatment
induces resistance in tumor cells. Conversely, it appears that Na[trans-RuCl4(DMSO)lm] may select a
tumor cell population which maintains its capacity to metastasise to the lung but with enhanced
sensitivity to the antimetastatic properties of this compound.
The Incidence of Lung Metastases in C3h Mice after Treatment of Implanted Solid Tumours with X-rays or Surgery