scholarly journals A novel method of generating RNAi library for high throughput gene function analysis in creeping bentgrass ( Agrostis stolonifera L.)

itsrj ◽  
2021 ◽  
Author(s):  
Jingjie Hao ◽  
Conglie Ma ◽  
Yanhai Yin ◽  
Shui‐zhang Fei
Keyword(s):  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Creeping Bentgrass ◽  
Agrostis Stolonifera ◽  
Novel Method ◽  
Gene Function Analysis

scholarly journals Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii

Parasitology ◽  
2014 ◽  
Vol 141 (11) ◽  
pp. 1390-1398 ◽  
Author(s):  
ELENA JIMÉNEZ-RUIZ ◽  
ELEANOR H. WONG ◽  
GURMAN S. PALL ◽  
MARKUS MEISSNER
Keyword(s):  
Toxoplasma Gondii ◽  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Advantages And Disadvantages ◽  
Gene Function Analysis ◽  
Tetracycline Inducible System

SUMMARYThe dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput.

scholarly journals High-throughput Binary Vectors for Plant Gene Function Analysis

2007 ◽  
Vol 49 (4) ◽  
pp. 556-567 ◽  
Author(s):  
Zhi-Yong Lei ◽  
Ping Zhao ◽  
Min-Jie Cao ◽  
Rong Cui ◽  
Xi Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Binary Vectors ◽  
Plant Gene ◽  
Gene Function Analysis

Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis

2008 ◽  
Vol 392 (3) ◽  
pp. 405-408 ◽  
Author(s):  
Ellyana Njatawidjaja ◽  
Hiroo Iwata
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
Gene Function ◽  
Function Analysis ◽  
Gene Function Analysis

scholarly journals A cassava common mosaic virus vector for virus-induced gene silencing in cassava

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Decai Tuo ◽  
Peng Zhou ◽  
Pu Yan ◽  
Hongguang Cui ◽  
Yang Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Gene Function ◽  
Viral Vector ◽  
Function Analysis ◽  
Systemic Infection ◽  
Cdna Clones ◽  
Virus Induced Gene Silencing ◽  
Efficient Gene ◽  
Gene Function Analysis

Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.

Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis

2006 ◽  
Vol 33 (4) ◽  
pp. 347 ◽  
Author(s):  
Changchun Wang ◽  
Xinzhong Cai ◽  
Xuemin Wang ◽  
Zhong Zheng
Keyword(s):  
Gene Silencing ◽  
High Throughput ◽  
Function Analysis ◽  
Tobacco Rattle Virus ◽  
Phytoene Desaturase ◽  
Growth Stages ◽  
Virus Induced Gene Silencing ◽  
Gene Functions ◽  
Genes Encoding ◽  
Gene Function Analysis

Arabidopsis thaliana (L.) Heynh. is a model plant species in which to study plant gene functions. Recently developed virus-induced gene silencing (VIGS) offers a rapid and high-throughput technique platform for gene function analysis. In this paper we report optimisation of tobacco rattle virus (TRV)-induced gene silencing in Arabidopsis. The parameters potentially affecting the efficiency of VIGS in Arabidopsis were investigated. These included the concentration and pre-incubation of Agrobacterium inocula (agro-inocula), the concentration of acetosyringone included in agro-inocula, the Agrobacterium inoculation (agro-inoculation) method, the ecotypes and the growth stages of Arabidopsis plants for agro-inoculation, and the growth temperature of agro-inoculated plants. The optimised VIGS procedure involves preparing the agro-inocula with OD600 of 2.0, pre-incubating for 2 h in infiltration buffer containing 200 μm acetosyringone, agro-inoculating by vacuum infiltration, and growth of agro-inoculated plants at 22 −24°C. Following this procedure consistent and highly efficient VIGS was achieved for the genes encoding phytoene desaturase (PDS) and actin in Arabidopsis. The silencing phenotype lasts for at least 6 weeks, and is applicable in at least seven ecotypes, including Col-0, Cvi-0, Sd, Nd-1, Ws-0, Bay-0 and Ler. TRV-induced VIGS was expressed not only in leaves, but also in stems, inflorescences and siliques. However, VIGS was not transmissible through seed to the subsequent generation. The optimised procedure of the TRV-induced gene silencing should facilitate high-throughput functional analysis of genes in Arabidopsis.

scholarly journals Large-scale gene function analysis with the PANTHER classification system

2013 ◽  
Vol 8 (8) ◽  
pp. 1551-1566 ◽  
Author(s):  
Huaiyu Mi ◽  
Anushya Muruganujan ◽  
John T Casagrande ◽  
Paul D Thomas
Keyword(s):  
Gene Function ◽  
Classification System ◽  
Large Scale ◽  
Function Analysis ◽  
Gene Function Analysis

Large-scale gene function analysis in Candida albicans

2004 ◽  
Vol 12 (4) ◽  
pp. 157-161 ◽  
Author(s):  
Vincent M. Bruno ◽  
Aaron P. Mitchell
Keyword(s):  
Candida Albicans ◽  
Gene Function ◽  
Large Scale ◽  
Function Analysis ◽  
Gene Function Analysis

Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens

2011 ◽  
pp. 57-79 ◽  
Author(s):  
Basel Khraiwesh ◽  
Isam Fattash ◽  
M. Asif Arif ◽  
Wolfgang Frank
Keyword(s):  
Gene Function ◽  
Physcomitrella Patens ◽  
Function Analysis ◽  
Gene Function Analysis
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