Insulin-Like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media

2012 ◽  
Vol 113 (8) ◽  
pp. 2714-2720 ◽  
Author(s):  
Minoru Tomizawa ◽  
Fuminobu Shinozaki ◽  
Takao Sugiyama ◽  
Shigenori Yamamoto ◽  
Makoto Sueishi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Factor I ◽  
Growth Factor I ◽  
Free Media

Lanreotide Reduces Serum Free and Total Insulin-Like Growth Factor-I After Angioplasty

Circulation ◽  
1996 ◽  
Vol 94 (10) ◽  
pp. 2465-2471 ◽  
Author(s):  
Jan Frystyk ◽  
Christian Skjærbæk ◽  
Niels Alexander ◽  
Håkan Emanuelsson ◽  
Harry Suryapranata ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Factor I ◽  
Growth Factor I

scholarly journals Induction of proliferation or hypertrophy of chondrocytes in serum-free culture: the role of insulin-like growth factor-I, insulin, or thyroxine.

1992 ◽  
Vol 116 (4) ◽  
pp. 1035-1042 ◽  
Author(s):  
K Böhme ◽  
M Conscience-Egli ◽  
T Tschan ◽  
K H Winterhalter ◽  
P Bruckner
Keyword(s):  
Growth Factor ◽  
Culture Media ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Matrix Deposition ◽  
Chondrocyte Maturation ◽  
Factor I ◽  
Growth Factor I ◽  
Serum Free Conditions

In bone forming cartilage in vivo, cells undergo terminal differentiation, whereas most of the cells in normal articular cartilage do not. Chondrocyte hypertrophy can be induced also in vitro by diffusible signals. We have identified growth factors or hormones acting individually on 17-d chick embryo sternal chondrocytes cultured in agarose gels under strictly serum-free conditions. Insulin-like growth factor I or insulin triggered the first steps of chondrocyte maturation, i.e., cell proliferation and increased matrix deposition while the chondrocytic phenotype was maintained. However, cells did not progress to the hypertrophic stage. Proliferation and stimulated collagen production was preceded by a lag period, indicating that synthesis of other components was required before cells became responsive to insulin-like growth factor I or insulin. Very small amounts of FBS exerted effects similar to those of insulin-like growth factor I or insulin. However, FBS could act directly and elicited hypertrophy when constituting greater than 1% of the culture media. Basic FGF has been claimed to be the most potent chondrocyte mitogen, but had negligible effects under serum-free conditions. The same is true for PDGF, a major serum-mitogen. Under the direction of thyroxine, cells did not proliferate but became typical hypertrophic chondrocytes, extensively synthesizing collagen X and alkaline phosphatase.

scholarly journals Serum-free insulin-like growth factor I correlates with clearance in patients with chronic renal failure

1999 ◽  
Vol 56 (6) ◽  
pp. 2076-2084 ◽  
Author(s):  
Jan Frystyk ◽  
Per Ivarsen ◽  
Christian Skjærbæk ◽  
Allan Flyvbjerg ◽  
Erling Bjerregaard Pedersen ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Factor I ◽  
Free Insulin ◽  
Growth Factor I

scholarly journals Maternal-conceptus signalling during early pregnancy in mares: oestrogen and insulin-like growth factor I

Reproduction ◽  
2001 ◽  
pp. 331-338 ◽  
Author(s):  
KW Walters ◽  
JF Roser ◽  
GB Anderson
Keyword(s):  
Growth Factor ◽  
Early Pregnancy ◽  
Culture Medium ◽  
Tissue Homogenate ◽  
Insulin Like Growth Factor ◽  
Serum Free ◽  
Paracrine Signalling ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Embryonic production of oestrogen is thought to play an important role in conceptus-maternal signalling during early pregnancy in mares, and may be regulated in an autocrine or paracrine fashion by insulin-like growth factor I (IGF-I). In this study, the hypothesis that IGF-I stimulates embryonic oestrogen synthesis, which in turn stimulates uterine IGF-I secretion was tested. Specific sources of IGF-I in the uterine lumen were characterized. Preimplantation embryos, uterine biopsies, and uterine flush fluids were collected on day 13 of pregnancy. Embryos were cultured whole for 24 h, or dispersed and incubated in serum-free culture medium supplemented with androstenedione or testosterone (0-10 microg ml(-1)) and IGF-I (0-100 microg ml(-1)). Oestrogen synthesis was increased by addition of androgen, but there was no dose-dependent effect of IGF-I. Endometrial explants were cultured for 24, 48 and 72 h in serum-free medium supplemented with oestradiol. IGF-I was measured by radioimmunoassay in embryo-conditioned medium, explant culture medium, blastocoelic fluid, concentrated (x 100) uterine flush fluid and endometrial-tissue homogenate. Both the embryo and endometrium produced significant quantities of IGF-I, indicating a role for this growth factor in autocrine-paracrine signalling during early pregnancy. However, secretion of IGF-I by endometrial explants was not modulated by oestrogen.

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