MicroRNA-122 Overexpression Promotes Hepatic Differentiation of Human Adipose Tissue-Derived Stem Cells

2014 ◽  
Vol 115 (9) ◽  
pp. 1582-1593 ◽  
Author(s):  
Nahid Davoodian ◽  
Abbas S. Lotfi ◽  
Masoud Soleimani ◽  
Seyed Javad Mowla
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation

Let-7f microRNA negatively regulates hepatic differentiation of human adipose tissue-derived stem cells

2014 ◽  
Vol 70 (3) ◽  
pp. 781-789 ◽  
Author(s):  
Nahid Davoodian ◽  
Abbas S. Lotfi ◽  
Masoud Soleimani ◽  
Seyed Javad Mola ◽  
Sare Arjmand
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation

Development of Simple Protocol for Generation of Functionally Active Hepatocyte-like Cells from Human Adipose Tissue-derived Stem Cells

2019 ◽  
Author(s):  
Sahere Rouzbehan ◽  
Nahid Davoodian ◽  
Ali Jamshidi ◽  
Ali Atashabparvar ◽  
Najmeh Davoodian
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Culture Conditions ◽  
Alpha Fetoprotein ◽  
Oncostatin M ◽  
Hepatic Differentiation ◽  
Differentiated Cells ◽  
Simple Protocol

Background and Aims: Human adipose tissue-derived stem cells (hASCs) are considered as an attractive source of regenerative stem cells, mainly because of their higher proliferation rate, more accessibility and hepatocyte like properties as compared to mesenchymal stem cells isolated from other tissues. Numerous studies have described the beneficial use of adipose tissue-derived stem cells for generating hepatocyte-like cells. However, due to the lack of appropriate culture conditions, most of the produced cells exhibit poor functionality. The aim of the present study was to establish a new protocol for the efficient hepatic differentiation of hASCs. Materials and Methods: hASCs were cultured in hepatic differentiation medium containing fibroblast growth factor 4, hepatocyte growth factor, dexamethasone and oncostatin M using a three-step protocol up to 21 days. Then, the functionality of the treated cells was evaluated by analyzing specific hepatocyte genes and biochemical markers at various time points. Results: A significant upregulation in albumin, alpha-fetoprotein, cytokeratin 18 and hepatocyte nuclear factor-4α expressions was observed in differentiated cells relative to day 1 of differentiation protocol. Moreover, the finding of glycogen deposits increased urea production and positive immunofluorescence staining for albumin and alpha-fetoprotein in hepatocyte-like cells suggesting that most of the cells differentiate into hepatocyte-like cells. Conclusions: Our report has provided a simple protocol for differentiation of hASCs into more functional hepatocyte-like cells.

Hepatic differentiation of human adipose tissue-derived mesenchymal stem cells and adverse effects of arsanilic acid and acetaminophen during in vitro hepatic developmental stage

2015 ◽  
Vol 31 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Mi-Jeong Kwon ◽  
Seok-Jin Kang ◽  
Young-Il Park ◽  
Yool-Hee Yang ◽  
Sa-Ik Bang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Adverse Effects ◽  
Developmental Stage ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation ◽  
Arsanilic Acid

Insulin‐like growth factor‐I promotes hepatic differentiation of human adipose tissue‐derived stem cells

2019 ◽  
Vol 43 (5) ◽  
pp. 476-485 ◽  
Author(s):  
Zahra Shabani Azandaryani ◽  
Nahid Davoodian ◽  
Afshin Samiei ◽  
Sahere Rouzbehan
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Insulin Like Growth Factor ◽  
Hepatic Differentiation ◽  
Factor I ◽  
Growth Factor I

scholarly journals Comparative study of BMP-2 gene delivery to Human adipose tissue-derived mesenchymal stem cells with Turbofect and Polyethylenimine

2021 ◽  
Vol 677 (4) ◽  
pp. 042024
Author(s):  
I A Nedorubova ◽  
T B Bukharova ◽  
A V Vasilyev ◽  
M A Syachina ◽  
D V Goldshtein ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Gene Delivery ◽  
Comparative Study ◽  
Human Adipose Tissue

Modification of Collagen Formation by Mesenchymal Stem Cells Isolated from Human Adipose Tissue in Culture and after Autotransplantation for Abdominal Hernia Plasty

2013 ◽  
Vol 156 (1) ◽  
pp. 152-155 ◽  
Author(s):  
V. G. Bogdan ◽  
M. M. Zafranskaya ◽  
Yu. M. Gain ◽  
Yu. E. Demidchik ◽  
S. S. Bagatka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Abdominal Hernia ◽  
Collagen Formation

Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo

Gut ◽  
2008 ◽  
Vol 58 (4) ◽  
pp. 570-581 ◽  
Author(s):  
H Aurich ◽  
M Sgodda ◽  
P Kaltwasser ◽  
M Vetter ◽  
A Weise ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatocyte Differentiation ◽  
Adipose Tissue In Vitro

Prostaglandins Differentially Affect Osteogenic Differentiation of Human Adipose Tissue–Derived Mesenchymal Stem Cells

2007 ◽  
Vol 13 (10) ◽  
pp. 2495-2503 ◽  
Author(s):  
M. Knippenberg ◽  
M.N. Helder ◽  
J.M.A. de Blieck-Hogervorst ◽  
P.I.J.M. Wuisman ◽  
J. Klein-Nulend
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Human Adipose Tissue

Transplantation of insulin-secreting cells differentiated from human adipose tissue-derived stem cells into type 2 diabetes mice

2014 ◽  
Vol 443 (2) ◽  
pp. 775-781 ◽  
Author(s):  
Ji Sun Nam ◽  
Hyun Mi Kang ◽  
Jiyoung Kim ◽  
Seah Park ◽  
Haekwon Kim ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Diabetes Mice ◽  
Insulin Secreting Cells

scholarly journals MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase

2019 ◽  
Vol 60 (12) ◽  
pp. 1187
Author(s):  
Yuting Wang ◽  
Lixin Lin ◽  
Yong Huang ◽  
Junjun Sun ◽  
Xueming Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Adipogenic Differentiation ◽  
Human Adipose Tissue
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