Neutral amino acid transport in embryonal carcinoma cells

1985 ◽  
Vol 122 (3) ◽  
pp. 379-386 ◽  
Author(s):  
John S. Zuzack ◽  
Richard J. Tasca ◽  
Stephen M. DiZio
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Neutral Amino Acid ◽  
Embryonal Carcinoma Cells ◽  
Acid Transport

scholarly journals Basic and Neutral Amino Acid Transport in Aspergillus nidulans

1976 ◽  
Vol 92 (1) ◽  
pp. 89-96 ◽  
Author(s):  
M. PIOTROWSKA ◽  
P. P. STEPIEN ◽  
E. BARTNIK ◽  
P. STEPIEN ◽  
E. BARTNIK ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aspergillus Nidulans ◽  
Amino Acid Transport ◽  
Neutral Amino Acid ◽  
Acid Transport

scholarly journals Electrogenic Na+-dependentl-alanine transport in the lizard duodenum. Involvement of systems A and ASC

2001 ◽  
Vol 280 (3) ◽  
pp. R612-R622
Author(s):  
Virtudes Medina ◽  
Antonio Lorenzo ◽  
Mario Dı́az
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Short Circuit ◽  
Duodenal Mucosa ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
Transport Activity ◽  
System A ◽  
Alanine Transport ◽  
Gallotia Galloti

l-Alanine transport across the isolated duodenal mucosa of the lizard Gallotia galloti has been studied in Ussing chambers under short-circuit conditions. Net l-alanine fluxes, transepithelial potential difference (PD), and short-circuit current ( Isc) showed concentration-dependent relationships. Na+-dependent l-alanine transport was substantially inhibited by the analog α-methyl aminoisobutyric acid (MeAIB). Likewise, MeAIB fluxes were completely inhibited byl-alanine, indicating the presence of system A for neutral amino acid transport. System A transport activity was electrogenic and exhibited hyperbolic relationships for net MeAIB fluxes, PD, and Isc, which displayed similar apparent K m values. Na+-dependentl-alanine transport, but not MeAIB transport, was partially inhibited by l-serine and l-cysteine, indicating the participation of system ASC. This transport activity represents the major pathway for l-alanine absorption and seemed to operate in an electroneutral mode with a negligible contribution to the l-alanine-induced electrogenicity. It is concluded from the present study that the active Na+-dependent l-alanine transport across the isolated duodenal mucosa of Gallotia galloti results from the independent activity of systems A and ASC for neutral amino acid transport.

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