Practical approach to protein recovery by countercurrent distribution in aqueous two-phase systems

PEG/ ammonium sulfate aqueous two-phase systems were supplied to separate GMP. It was found that PEG molecular weight, PEG concentration and ammonium sulfate concentration could influence the partitioning of GMP. The data of experiment showed that 18% (w/w) PEG6000 and 15% (w/w) ammonium sulfate were the optimum system, in which the protein recovery was 69.2%

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Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems

Cellular Immunology ◽

10.1016/0008-8749(78)90210-1 ◽

1978 ◽

Vol 37 (2) ◽

pp. 422-431 ◽

Cited By ~ 9

Author(s):

K. Nelson ◽

P. Malmström ◽

Å. Jönsson ◽

H.O. Sjögren

Keyword(s):

Countercurrent Distribution ◽

Two Phase ◽

Aqueous Two Phase Systems ◽

Phase Systems

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Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems

Cellular Immunology ◽

10.1016/0008-8749(78)90209-5 ◽

1978 ◽

Vol 37 (2) ◽

pp. 409-421 ◽

Cited By ~ 21

Author(s):

P. Malmström ◽

K. Nelson ◽

Å. Jönsson ◽

H.O. Sjögren ◽

H. Walter ◽

...

Keyword(s):

Countercurrent Distribution ◽

Two Phase ◽

Aqueous Two Phase Systems ◽

Phase Systems

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Studies of protein—Protein interaction using countercurrent distribution in aqueous two-phase systems: Partition behavior of five glycolytic enzymes from crude baker's yeast extract

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(90)90030-3 ◽

1990 ◽

Vol 276 (1) ◽

pp. 227-231 ◽

Cited By ~ 2

Author(s):

Lars-Olof Persson ◽

Göte Johansson

Keyword(s):

Yeast Extract ◽

Protein Interaction ◽

Glycolytic Enzymes ◽

Countercurrent Distribution ◽

Baker's Yeast ◽

Two Phase ◽

Protein Protein Interaction ◽

Aqueous Two Phase Systems ◽

Phase Systems ◽

Partition Behavior

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Studies of protein-protein interaction using countercurrent distribution in aqueous two-phase systems. Partition behaviour of six Calvin-cycle enzymes from a crude spinach (Spinacia oleracea) chloroplast extract

Biochemical Journal ◽

10.1042/bj2590863 ◽

1989 ◽

Vol 259 (3) ◽

pp. 863-870 ◽

Cited By ~ 19

Author(s):

L O Persson ◽

G Johansson

Keyword(s):

Protein Interaction ◽

Spinacia Oleracea ◽

Calvin Cycle ◽

Phosphoglycerate Kinase ◽

Countercurrent Distribution ◽

Two Phase ◽

Protein Protein Interaction ◽

Aqueous Two Phase Systems ◽

Phase Systems ◽

Partition Behaviour

The partition behaviour of six enzymes of the Calvin cycle in extracts of chloroplasts from spinach (Spinacia oleracea) between two aqueous phases has been studied by countercurrent distribution. The enzymes showed distribution patterns which indicate heterogeneity and the presence of two or three fractions of most of the enzymes. When two of the enzymes, phosphoglycerate kinase and fructose-bisphosphate aldolase, were partitioned in both purified and partially purified form, they behaved like homogeneous substances. These results indicate that countercurrent distribution of crude extracts in aqueous two-phase systems is a useful method to study protein-protein interaction.

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