Effect of COD/N and COD/P ratios on the PHA transformation and dynamics of microbial community structure in a denitrifying phosphorus removal process

2012 ◽  
Vol 88 (7) ◽  
pp. 1228-1236 ◽  
Author(s):  
Yayi Wang ◽  
Junjun Geng ◽  
Zhongjia Ren ◽  
Gang Guo ◽  
Chong Wang ◽  
...  

2007 ◽  
Vol 41 (19) ◽  
pp. 4383-4396 ◽  
Author(s):  
Gilda Carvalho ◽  
Paulo C. Lemos ◽  
Adrian Oehmen ◽  
Maria A.M. Reis


2011 ◽  
Vol 378-379 ◽  
pp. 428-432
Author(s):  
Yu Qin ◽  
Jing Song Guo ◽  
Fang Fang

PCR-DGGE was applied to analyze the relationship between pH and the microbial community structure of Sequence Batch Biofilm Reactor (SBBR) autotrophic nitrogen removal process. The reactor was possessed of a high nitrogen removal efficiency at pH=8.0 where the similarity of microbial community structure between active sludge and biofilm samples was the lowest about 84.6% and the richness of bacterial community was the most abundant in biofilm compared with other pH conditions. pH=7.0 was good for the microbes in active sludge but unfavorable for anaerobic bacteria. At pH=9.0, the effects were presented with both bacterial activities and microbial community structure and when pH=6.0 the amount of microbial types dramatically dropped





2011 ◽  
Vol 77 (22) ◽  
pp. 7942-7953 ◽  
Author(s):  
J. P. Bassin ◽  
M. Pronk ◽  
G. Muyzer ◽  
R. Kleerebezem ◽  
M. Dezotti ◽  
...  

ABSTRACTThe long- and short-term effects of salt on biological nitrogen and phosphorus removal processes were studied in an aerobic granular sludge reactor. The microbial community structure was investigated by PCR-denaturing gradient gel electrophoresis (DGGE) on 16S rRNA andamoAgenes. PCR products obtained from genomic DNA and from rRNA after reverse transcription were compared to determine the presence of bacteria as well as the metabolically active fraction of bacteria. Fluorescencein situhybridization (FISH) was used to validate the PCR-based results and to quantify the dominant bacterial populations. The results demonstrated that ammonium removal efficiency was not affected by salt concentrations up to 33 g/liter NaCl. Conversely, a high accumulation of nitrite was observed above 22 g/liter NaCl, which coincided with the disappearance ofNitrospirasp. Phosphorus removal was severely affected by gradual salt increase. No P release or uptake was observed at steady-state operation at 33 g/liter NaCl, exactly when the polyphosphate-accumulating organisms (PAOs), “CandidatusAccumulibacter phosphatis” bacteria, were no longer detected by PCR-DGGE or FISH. Batch experiments confirmed that P removal still could occur at 30 g/liter NaCl, but the long exposure of the biomass to this salinity level was detrimental for PAOs, which were outcompeted by glycogen-accumulating organisms (GAOs) in the bioreactor. GAOs became the dominant microorganisms at increasing salt concentrations, especially at 33 g/liter NaCl. In the comparative analysis of the diversity (DNA-derived pattern) and the activity (cDNA-derived pattern) of the microbial population, the highly metabolically active microorganisms were observed to be those related to ammonia (Nitrosomonassp.) and phosphate removal (“CandidatusAccumulibacter”).





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