scholarly journals Effects of ammonia on propionate degradation and microbial community in digesters using propionate as a sole carbon source

2017 ◽  
Vol 92 (10) ◽  
pp. 2538-2545 ◽  
Author(s):  
Ying Li ◽  
Yue Zhang ◽  
Xiaoying Kong ◽  
Lianhua Li ◽  
Zhenhong Yuan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Propionate Degradation

scholarly journals Influence of Liquid-to-Gas Ratio on the Syngas Fermentation Efficiency: An Experimental Approach

2020 ◽  
Vol 7 (4) ◽  
pp. 138
Author(s):  
Spyridon Achinas ◽  
Sytse Jelmer Mulder ◽  
Gerrit Jan Willem Euverink
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Experimental Approach ◽  
Complete Conversion ◽  
Fed Batch ◽  
Syngas Fermentation ◽  
Fermentation Efficiency ◽  
Anaerobic Microbial Community ◽  
Gas Ratio

Syngas fermentation by methanogens is a novel process to purify biogas. Methanogens are able to ferment non-desirable CO2, H2, and CO to methane. However, to use methanogens on an industrial scale, more research has to be done. There are studies that discuss the growth of methanogens on syngas in combination with acetate. In this research, growth of methanogens on syngas as sole carbon source is discussed. Effluent of an anaerobic fed-batch was selectively cultivated with syngas in 400 mL Eppendorf© bioreactors. After a period of 7 days, fifteen 120 mL flasks were filled with three different liquid-to-gas ratios (1:1, 1:3, 1:5). Results showed that different liquid-to-gas ratios change the metabolic preference of the anaerobic microbial community. Moreover, complete conversion in a four-to-eight-day period, via the carboxidotrophic pathway, was observed in all three liquid-to-gas ratios.

scholarly journals Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91977 ◽  
Author(s):  
Yifei Zhang ◽  
Yunfei Zheng ◽  
Jianwei Hu ◽  
Ning Du ◽  
Feng Chen
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Functional Diversity ◽  
Healthy Subjects ◽  
Sole Carbon Source ◽  
Carbon Source Utilization ◽  
Sole Carbon Source Utilization

scholarly journals Identification of Two prpDBC Gene Clusters in Corynebacterium glutamicum and Their Involvement in Propionate Degradation via the 2-Methylcitrate Cycle

2002 ◽  
Vol 184 (10) ◽  
pp. 2728-2739 ◽  
Author(s):  
Wilfried A. Claes ◽  
Alfred Pühler ◽  
Jörn Kalinowski
Keyword(s):  
Carbon Source ◽  
Corynebacterium Glutamicum ◽  
Sole Carbon Source ◽  
Citrate Synthase ◽  
Gene Clusters ◽  
Exponential Growth Phase ◽  
Gene Products ◽  
Two Dimensional Gel Electrophoresis ◽  
Propionate Degradation ◽  
Methylcitrate Synthase

ABSTRACT Genome sequencing revealed that the Corynebacterium glutamicum genome contained, besides gltA, two additional citrate synthase homologous genes (prpC) located in two different prpDBC gene clusters, which were designated prpD1B1C1 and prpD2B2C2. The coding regions of the two gene clusters as well as the predicted gene products showed sequence identities of about 70 to 80%. Significant sequence similarities were found also to the prpBCDE operons of Escherichia coli and Salmonella enterica, which are known to encode enzymes of the propionate-degrading 2-methylcitrate pathway. Homologous and heterologous overexpression of the C. glutamicum prpC1 and prpC2 genes revealed that their gene products were active as citrate synthases and 2-methylcitrate synthases. Growth tests showed that C. glutamicum used propionate as a single or partial carbon source, although the beginning of the exponential growth phase was strongly delayed by propionate for up to 7 days. Compared to growth on acetate, the specific 2-methylcitrate synthase activity increased about 50-fold when propionate was provided as the sole carbon source, suggesting that in C. glutamicum the oxidation of propionate to pyruvate occurred via the 2-methylcitrate pathway. Additionally, two-dimensional gel electrophoresis experiments combined with mass spectrometry showed strong induction of the expression of the C. glutamicum prpD2B2C2 genes by propionate as an additional carbon source. Mutational analyses revealed that only the prpD2B2C2 genes were essential for the growth of C. glutamicum on propionate as a sole carbon source, while the function of the prpD1B1C1 genes remains obscure.

scholarly journals Efficient Removal of Butachlor and Change in Microbial Community Structure in Single-Chamber Microbial Fuel Cells

2019 ◽  
Vol 16 (20) ◽  
pp. 3897
Author(s):  
Xiaojing Li ◽  
Yue Li ◽  
Lixia Zhao ◽  
Yang Sun ◽  
Xiaolin Zhang ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Community Structure ◽  
Microbial Community ◽  
Carbon Source ◽  
Microbial Community Structure ◽  
Microbial Fuel Cells ◽  
Sodium Acetate ◽  
Sole Carbon Source ◽  
High Throughput Sequencing ◽  
Single Chamber

Microbial electrochemical technology provides an inexhaustible supply of electron acceptors, allowing electroactive microorganisms to generate biocurrent and accelerate the removal of organics. The treatment of wastewater contaminated by butachlor, which is a commonly used chloroacetamide herbicide in paddy fields, is a problem in agricultural production. In this study, butachlor was found to be removed efficiently (90 ± 1%) and rapidly (one day) in constructed single-chamber microbial fuel cells (MFCs). After the addition of sodium acetate to MFCs with butachlor as the sole carbon source, electricity generation was recovered instead of increasing the degradation efficiency of butachlor. Meanwhile, the microbial community structure was changed in anodic and cathodic biofilms after the addition of butachlor, following the bioelectrochemical degradation of butachlor. High-throughput sequencing showed the proliferation of Paracoccus and Geobacter in MFCs with butachlor as the sole carbon source and of Thauera butanivorans in MFCs with butachlor and sodium acetate as concomitant carbon sources. These species possess the ability to oxidize different substituents of butachlor and have important potential use for the bioremediation of wastewater, sediments, and soils.

scholarly journals Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles – a critique

2002 ◽  
Vol 42 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Juliet Preston-Mafham ◽  
Lynne Boddy ◽  
Peter F. Randerson
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Functional Diversity ◽  
Sole Carbon Source ◽  
Carbon Source Utilisation

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

Performance enhancement of H2S-based autotrophic denitrification with bio-gaseous CO2 as sole carbon source through new pH adjustment materials

2020 ◽  
Vol 261 ◽  
pp. 110157 ◽  
Author(s):  
Yongjie Liu ◽  
Nan Chen ◽  
Shuang Tong ◽  
Jing Liang ◽  
Chen Yang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Performance Enhancement ◽  
Autotrophic Denitrification ◽  
Ph Adjustment

Woodchips bioretention column for stormwater treatment: Nitrogen removal performance, carbon source and microbial community analysis

Chemosphere ◽  
2021 ◽  
pp. 131519
Author(s):  
Lingjie Liu ◽  
Fen Wang ◽  
Sihan Xu ◽  
Wei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Nitrogen Removal ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Stormwater Treatment
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