Preparation of a poly ( PEGDA‐ co ‐GMA ) thin hydrogel matrix for oligonucleotide microarray applications

2021 ◽  
Author(s):  
Yuan Qi ◽  
Kaiyao Li ◽  
Changwen Zhao ◽  
Yuhong Ma ◽  
Wantai Yang
Keyword(s):  
Oligonucleotide Microarray ◽  
Hydrogel Matrix

Amplification-free detection of grapevine viruses using an oligonucleotide microarray

2011 ◽  
Vol 178 (1-2) ◽  
pp. 1-15 ◽  
Author(s):  
Ismail Abdullahi ◽  
Yana Gryshan ◽  
Michael Rott
Keyword(s):  
Oligonucleotide Microarray

scholarly journals DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 266
Author(s):  
Nataša Žuržul ◽  
Bjørn Torger Stokke
Keyword(s):  
Conformational Transition ◽  
Polymer Network ◽  
Potassium Ion ◽  
Ion Concentration ◽  
Dna Aptamer ◽  
Blood Levels ◽  
Label Free ◽  
Practical Applications ◽  
Hydrogel Matrix ◽  
K Concentration

In the present paper, we describe a potassium sensor based on DNA-aptamer functionalized hydrogel, that is capable of continuous label-free potassium ion (K+) monitoring with potential for in situ application. A hydrogel attached to the end of an optical fiber is designed with di-oligonucleotides grafted to the polymer network that may serve as network junctions in addition to the covalent crosslinks. Specific affinity toward K+ is based on exploiting a particular aptamer that exhibits conformational transition from single-stranded DNA to G-quadruplex formed by the di-oligonucleotide in the presence of K+. Integration of this aptamer into the hydrogel transforms the K+ specific conformational transition to a K+ concentration dependent deswelling of the hydrogel. High-resolution interferometry monitors changes in extent of swelling at 1 Hz and 2 nm resolution for the hydrogel matrix of 50 µm. The developed hydrogel-based biosensor displayed high selectivity for K+ ions in the concentration range up to 10 mM, in the presence of physiological concentrations of Na+. Additionally, the concentration dependent and selective K+ detection demonstrated in the artificial blood buffer environment, both at room and physiological temperatures, suggests substantial potential for practical applications such as monitoring of potassium ion concentration in blood levels in intensive care medicine.

scholarly journals Injection of a polymerized hyaluronic acid/collagen hydrogel matrix in an in vivo porcine disc degeneration model

2012 ◽  
Vol 21 (9) ◽  
pp. 1700-1708 ◽  
Author(s):  
G. W. Omlor ◽  
A. G. Nerlich ◽  
H. Lorenz ◽  
T. Bruckner ◽  
W. Richter ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Disc Degeneration ◽  
Collagen Hydrogel ◽  
Hydrogel Matrix

scholarly journals Enhanced oligonucleotide microarray labeling and hybridization

BioTechniques ◽  
2006 ◽  
Vol 41 (6) ◽  
pp. 685-686 ◽  
Author(s):  
Haider Ali Syed ◽  
David W. Threadgill
Keyword(s):  
Oligonucleotide Microarray

scholarly journals A New Oligonucleotide Microarray for Detection of Pathogenic and Non-Pathogenic Legionella spp.

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e113863 ◽  
Author(s):  
Boyang Cao ◽  
Xiangqian Liu ◽  
Xiang Yu ◽  
Min Chen ◽  
Lu Feng ◽  
...  
Keyword(s):  
Oligonucleotide Microarray

Mössbauer spectroscopy study of iron complexes in a poly(methacrylic acid) hydrogel matrix

2011 ◽  
Vol 47 (11) ◽  
pp. 1271-1274 ◽  
Author(s):  
L. V. Khenkin ◽  
A. I. Shishakov ◽  
A. A. Novakova ◽  
E. Yu. Kozhunova ◽  
E. E. Makhaeva
Keyword(s):  
Mössbauer Spectroscopy ◽  
Methacrylic Acid ◽  
Mossbauer Spectroscopy ◽  
Iron Complexes ◽  
Spectroscopy Study ◽  
Mößbauer Spectroscopy ◽  
Hydrogel Matrix

scholarly journals Discovery of 830 candidate therapeutic targets and diagnostic markers for breast cancer using oligonucleotide microarray technology

10.1038/87236 ◽  
2001 ◽  
Vol 27 (S4) ◽  
pp. 77-77 ◽  
Author(s):  
Michael Orr ◽  
Amanda Williams ◽  
Lisa Vogt ◽  
Joseph Boland ◽  
Harry Yang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Therapeutic Targets ◽  
Diagnostic Markers ◽  
Oligonucleotide Microarray ◽  
Microarray Technology

Transcriptomic analysis of floral organs from Phalaenopsis orchid by using oligonucleotide microarray

Gene ◽  
2013 ◽  
Vol 518 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Yu-Yun Hsiao ◽  
Tian-Hsiang Huang ◽  
Chih-Hsiung Fu ◽  
Shi-Ching Huang ◽  
Yi-Jun Chen ◽  
...  
Keyword(s):  
Oligonucleotide Microarray ◽  
Transcriptomic Analysis ◽  
Floral Organs
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