Monoclonal antibody against mouse sperm blocks a specific event in the fertilization process

1983 ◽  
Vol 227 (3) ◽  
pp. 481-486 ◽  
Author(s):  
P. M. Saling ◽  
L. M. Raines ◽  
M. G. O'rand
Keyword(s):  
Monoclonal Antibody ◽  
Mouse Sperm ◽  
Fertilization Process ◽  
Specific Event

scholarly journals Epididymal Secretion of a Mouse Sperm Surface Component Recognized by a Monoclonal Antibody

1982 ◽  
Vol 26 (3) ◽  
pp. 523-535 ◽  
Author(s):  
Robert B. Vernon ◽  
Charles H. Muller ◽  
John C. Herr ◽  
Frederick A. Feuchter ◽  
E. M. Eddy
Keyword(s):  
Monoclonal Antibody ◽  
Mouse Sperm ◽  
Surface Component ◽  
Sperm Surface

scholarly journals Distribution of Molecular Epitope for Ts4, an Anti-Sperm Auto-Monoclonal Antibody in the Fertilization Process

2009 ◽  
Vol 55 (3) ◽  
pp. 240-246 ◽  
Author(s):  
Yohei SHIRAI ◽  
Hiroshi YOSHITAKE ◽  
Mayuko MARUYAMA ◽  
Kenji TAKAMORI ◽  
Hideoki OGAWA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fertilization Process

scholarly journals Anti-Mouse Sperm Monoclonal Antibody, A-1, Inhibits Sperm Capacitation, Acrosome Reaction and Calcium Influx into Spermatocytes.

2000 ◽  
Vol 23 (8) ◽  
pp. 922-925 ◽  
Author(s):  
Yuichi KAWAI ◽  
Akira TAKEIRI ◽  
Takashi SUZUKI ◽  
Yasuo SUZUKI ◽  
Masaharu MIYAKE
Keyword(s):  
Monoclonal Antibody ◽  
Acrosome Reaction ◽  
Calcium Influx ◽  
Sperm Capacitation ◽  
Mouse Sperm

The Immunoreactivity of Ts4, an Anti-TEX101 Monoclonal Antibody, Derived from Anti-mouse Sperm Autoantibody.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 156-157
Author(s):  
Hiroshi Yoshitake ◽  
Atsuko Kamo ◽  
Mitsuaki Yanagida ◽  
Yohei Shirai ◽  
Kenji Takamori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mouse Sperm

scholarly journals Studies on sperm capacitation using monoclonal antibody - Disappearance of an antigen from the anterior part of mouse sperm head.

1986 ◽  
Vol 9 (1) ◽  
pp. 55-60 ◽  
Author(s):  
MASARU OKABE ◽  
KATSUAKI TAKADA ◽  
TOSHIYUKI ADACHI ◽  
YASUHIRO KOHAMA ◽  
TSUTOMU MIMURA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Anterior Part ◽  
Sperm Head ◽  
Sperm Capacitation ◽  
Mouse Sperm

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

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