A High-Yield, High-Purity Elutriation Method for Preparing Human Granulocytes Demonstrating Enhanced Experimental Lifetimes

1987 ◽  
Vol 42 (1) ◽  
pp. 43-50 ◽  
Author(s):  
James O. Tolley ◽  
Geneva M. Omann ◽  
Algirdas J. Jesaitis
Keyword(s):  
High Purity ◽  
High Yield ◽  
Human Granulocytes

scholarly journals A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2

2020 ◽  
Author(s):  
Cecy Xi ◽  
Arianna Arianna Di Fazio ◽  
Naveed Nadvi ◽  
Karishma Patel ◽  
Michelle Xiang ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
High Purity ◽  
Specific Activity ◽  
Specific Binding ◽  
Exchange Chromatography ◽  
Cell Surface Receptor ◽  
Spike Protein ◽  
High Yield ◽  
Purification Procedure ◽  
Dipeptidyl Peptidase 4

Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulfate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor binding domain (RBD) were measured using surface plasmon resonance. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.

scholarly journals Water-based synthesis and cleaning methods for high purity ZnO nanoparticles – comparing acetate, chloride, sulphate and nitrate zinc salt precursors

RSC Advances ◽  
2014 ◽  
Vol 4 (67) ◽  
pp. 35568-35577 ◽  
Author(s):  
A. M. Pourrahimi ◽  
D. Liu ◽  
L. K. H. Pallon ◽  
R. L. Andersson ◽  
A. Martínez Abad ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
High Purity ◽  
Zinc Salt ◽  
High Yield ◽  
Water Based ◽  
Zinc Salts ◽  
Precipitation Synthesis ◽  
Cleaning Methods

The effect of using different zinc salts on size, morphology and photoluminescence of ZnO nanoparticles in high-yield aqueous precipitation synthesis.

scholarly journals 3-(2-Aminophenyl)-4-methyl-1,3-thiazole-2(3H)-thione as an Ecofriendly Sulphur Transfer Agent to Prepare Alkanethiols in High Yield and High Purity

Molecules ◽  
2009 ◽  
Vol 14 (11) ◽  
pp. 4634-4643 ◽  
Author(s):  
Mohammed Amine Mehdid ◽  
Ayada Djafri ◽  
Christian Roussel ◽  
Federico Andreoli
Keyword(s):  
High Purity ◽  
High Yield

ChemInform Abstract: A Facile New Synthesis of Aldehyde Enamines in High Yield and High Purity.

ChemInform ◽  
2010 ◽  
Vol 25 (49) ◽  
pp. no-no
Author(s):  
G. B. FISHER ◽  
L. LEE ◽  
F. W. KLETTKE
Keyword(s):  
High Purity ◽  
High Yield ◽  
New Synthesis

scholarly journals High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Andrea Maria Summerauer ◽  
Lorenzo Colombo ◽  
Rodney Ogwang ◽  
Christoph Berger ◽  
Jan Fehr ◽  
...  
Keyword(s):  
High Purity ◽  
Cell Isolation ◽  
T Helper Cell ◽  
Helper Cell ◽  
High Yield ◽  
Pcr Analysis ◽  
T Helper ◽  
Qrt Pcr

scholarly journals A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5392
Author(s):  
Cecy R Xi ◽  
Arianna Di Fazio ◽  
Naveed Ahmed Nadvi ◽  
Karishma Patel ◽  
Michelle Sui Wen Xiang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Affinity Chromatography ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Purity ◽  
Specific Activity ◽  
Cell Surface Receptor ◽  
Spike Protein ◽  
High Yield ◽  
Purification Procedure

Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of the MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29–766) produced in insect cells. Purification used differential ammonium sulphate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion-exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected by surface plasmon resonance or ELISA. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.

The Preparation of Silver Nanowires and the Study of SERS Activity of Single Nanowire

2012 ◽  
Vol 535-537 ◽  
pp. 384-387 ◽  
Author(s):  
Hai Yan Li ◽  
Mei Jie Dang ◽  
Pei Jie Wang ◽  
Yan Fang
Keyword(s):  
High Purity ◽  
Silver Nanowires ◽  
High Yield ◽  
Excitation Wavelength ◽  
Enhancement Effect ◽  
Improved Method ◽  
Absorption Bands ◽  
Single Nanowire ◽  
Surface Enhanced Raman ◽  
Longitudinal Plasma

In this report, the polyol process was used for preparing silver nanowires. In this improved method, the silver nanowires with high purity, high yield and high sensitive surface enhanced Raman scattering (SERS) activity were synthesized mainly by sealed heating. Then the samples were characterized by scanning electron microscopy (SEM). Its optical absorption properties were measured by UV-Vis spectrophotometer; furthermore, SERS spectra of R6G molecule on the single nanowire was obtained and systematically studied by excitation wavelength 532nm. Our experimental results showed that the length of nanowires by our improved method is uniform and has high yield, high purity and higher SERS enhancement effect. The UV-Vis absorption spectrum of the samples displayed the transverse and longitudinal plasma resonance (SPR) absorption bands of silver nanowires which are located at 358nm and 416nm, respectively. Also single nanowire SERS activities which are dependent on the different substrates were researched.

scholarly journals High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers

2015 ◽  
Vol 10 (2) ◽  
pp. 305-315 ◽  
Author(s):  
Ingmar Mederacke ◽  
Dianne H Dapito ◽  
Silvia Affò ◽  
Hiroshi Uchinami ◽  
Robert F Schwabe
Keyword(s):  
High Purity ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
High Yield
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