The Effects of 16,16-Dimethyl PGE2 and Phosphodiesterase Inhibitors on Con A Blastogenic Responses and NK Cytotoxic Activity of Mouse Spleen Cells

1990 ◽  
Vol 47 (4) ◽  
pp. 371-377 ◽  
Author(s):  
N.K. Ellis ◽  
G.P. Duffie ◽  
M.R. Young ◽  
H.T. Wepsic
Keyword(s):  
Cytotoxic Activity ◽  
Phosphodiesterase Inhibitors ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Con A

scholarly journals POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS

1972 ◽  
Vol 136 (1) ◽  
pp. 143-155 ◽  
Author(s):  
Igal Gery ◽  
Byron H. Waksman
Keyword(s):  
T Cells ◽  
Bone Marrow Cells ◽  
Mouse Spleen ◽  
Adherent Cells ◽  
Spleen Cells ◽  
Con A ◽  
Cell Responses ◽  
Donor Cells ◽  
Peripheral Leukocytes ◽  
Nonadherent Cells

Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced after stimulation. Lipopolysaccharide (LPS) produced very active SUP in all cultures tested. PHA was similarly active on human leukocytes only, whereas concanavalin A (Con A) gave highly efficient SUP only with mouse spleen cells. SUP production is not correlated with a mitotic response of the donor cells and is observed in cultures unable to respond mitotically to the stimulant. Adherent mouse spleen cell populations, consisting largely or entirely of macrophages, produce active SUP, while nonadherent cells do not. Similarly, purification of human peripheral leukocytes on nylon columns, with removal of macrophages and other adherent cells, destroys their ability to produce SUP. The importance of indirect effects in stimulating mitotic responses of T cells is emphasized by the fact that the mitotic response of mouse thymocytes to LPS and its ability to potentiate the response of these cells to PHA disappears with removal of adherent cells from the thymocyte population. Conversely the production of SUP from spleen cells stimulated by Con A requires the presence of T cells.

scholarly journals GENETIC CONTROL OF THYMUS-DERIVED CELL FUNCTION

1972 ◽  
Vol 135 (6) ◽  
pp. 1279-1292 ◽  
Author(s):  
R. Michael Williams ◽  
Baruj Benacerraf
Keyword(s):  
Autosomal Dominant ◽  
Cell Function ◽  
Inbred Strains ◽  
Mouse Spleen ◽  
C Cells ◽  
Spleen Cells ◽  
Con A ◽  
Polygenic Control ◽  
Better Than

Concanavalin A- or phytohemagglutinin-stimulated DNA synthetic responses of 1 million normal mouse spleen cells in vitro were significantly different among various inbred strains. BALB/cJ (H-2d) responded better than C57BL/6J (H-2b) spleen cells, and the responses of C3H/HeJ or AKR/J (both H-2k) cells were intermediate. These responses, measured as the increment in thymidine-3H incorporation of mitogen-stimulated compared with unstimulated cultures, varied according to the number of cells cultured or the mitogen concentration. BALB/c spleens had the highest proportion of θ-positive cells, but no direct relationship between the proportion of θ-positive cells and the DNA synthetic response was observed. (BALB/cJ x C57BL/6)F1 spleen cells responsed as well as BALB/c cells. Responses of spleen cells from (F1 x C57BL/6) backcross littermates varied over a range equal to, or greater than, that of BALB/c and C57BL/6 cells. There was no correlation between H-2 specificity (H-2bd or H-2bb) or sex and the mitogen-stimulated DNA synthetic response of backcross animals. Con A- and PHA-stimulated responses of individual backcross animals were positively correlated with the level of thymidine-8H incorporation by unstimulated spleen cells. These results are consistent with autosomal dominant, non-H-2-linked, polygenic control of the mitogen-stimulated in vitro DNA synthetic response of mouse spleen cells.

Analysis of changes in natural cytostatic and cytotoxic activity of mouse spleen cells after administration of cyclophosphamide and ?-interferon

1986 ◽  
Vol 102 (2) ◽  
pp. 1099-1102
Author(s):  
L. V. Van'ko ◽  
I. V. Bogdashin ◽  
G. T. Sukhikh ◽  
N. V. Totibadze ◽  
B. B. Fuks
Keyword(s):  
Cytotoxic Activity ◽  
Mouse Spleen ◽  
Spleen Cells

Differential effect of low molecular weight alcohols on the Con A stimulation of mouse spleen cells

1982 ◽  
Vol 4 (6) ◽  
pp. 305-309 ◽  
Author(s):  
Astrid Raile ◽  
Klaus P. Hammann ◽  
Otto Scheiner ◽  
Thomas Schulz ◽  
Anna Erdei ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Differential Effect ◽  
Mouse Spleen ◽  
Low Molecular Weight ◽  
Spleen Cells ◽  
Con A ◽  
Stimulation Of

Regulation of β-endorphin receptor expression in mouse spleen cells with con A and rIL-2

1992 ◽  
Vol 14 (5) ◽  
pp. 809-819 ◽  
Author(s):  
Ling Jia ◽  
Hideki Hara ◽  
Toshikazu Okochi ◽  
Shigeru Negoro
Keyword(s):  
Receptor Expression ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Con A

scholarly journals Specific elimination of cytotoxic effector cells. I. Adsorptive behavior of effectors and their precursors and spleen cell monolayers.

1976 ◽  
Vol 144 (4) ◽  
pp. 996-1008 ◽  
Author(s):  
J R Neefe ◽  
D H Sachs
Keyword(s):  
Mouse Spleen ◽  
Spleen Cells ◽  
Effector Cells ◽  
Cell Monolayers ◽  
Specific Suppression ◽  
Normal Spleen ◽  
Adsorptive Behavior ◽  
Cytotoxic Effector ◽  
Primed Mouse

Monolayers formed of normal mouse spleen cells attached to polystyrene coated with poly-L-lysine were tested for their ability to bind specifically antigen-reactive cells in normal or primed mouse spleen. 88 to greater than 98% of the activity of cytotoxic populations was removed by a single adsorption. However, normal spleen cells or spleen cells previously primed in vitro could not be depleted of their capacity to be sensitized, even when adsorption effectively removed all residual cytotoxic activity from the same previously primed population. In fact, exposure to an immunoadsorbent augmented the ultimate cytotoxicity generated in a nonspecific fashion. This augmentation was especially dramatic in the case of a previously primed population and may have reflected the removal of a nonspecific suppressor. If antigen-reactive precursors cannot be removed efficiently by adsorption, other approaches to the generation of tolerant lymphoid populations, such as specific suppression of precursor differentiation must be sought.

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