Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens

A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira interrogans, Listeria monocytogenes, lymphocytic choriomeningitis virus, measles virus, mumps virus, Mycobacterium sp., Mycoplasma pneumoniae, Toxoplasma gondii and varicella zoster virus. Of the 383 specimens tested in this study, 46 (12.0%) were found to be positive. The microorganisms detected were CMV, enterovirus, Epstein Barr virus, herpes simplex virus, human herpes virus type 6, JC virus, L. monocytogenes, Mycobacterium genus, Toxoplasma gondii and varicella zoster virus. The introduction of the PCR protocol described has improved the diagnosis of a range of central nervous system infections in our laboratory. We believe however that further evaluation of these assays in immunocompromised patients is necessary to better determine the predictive value of positive PCR results in these patient groups.

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Simultaneous Measurement of Antibodies to Epstein-Barr Virus, Human Herpesvirus 6, Herpes Simplex Virus Types 1 and 2, and 14 Enteroviruses in Chronic Fatigue Syndrome: Is There Evidence of Activation of a Nonspecific Polyclonal Immune Response?

Clinical Infectious Diseases ◽

10.1093/clinids/19.3.448 ◽

1994 ◽

Vol 19 (3) ◽

pp. 448-453 ◽

Cited By ~ 16

Author(s):

F. A. Manian

Keyword(s):

Immune Response ◽

Herpes Simplex ◽

Epstein Barr Virus ◽

Human Herpesvirus ◽

Chronic Fatigue ◽

Human Herpesvirus 6 ◽

Barr Virus ◽

Fatigue Syndrome ◽

Epstein Barr ◽

Simplex Virus

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Mechanisms of Viral Infections Associated with HIV

Advances in Dental Research ◽

10.1177/0022034511400076 ◽

2011 ◽

Vol 23 (1) ◽

pp. 130-136 ◽

Cited By ~ 4

Author(s):

S.M. Tugizov ◽

J.Y. Webster-Cyriaque ◽

S. Syrianen ◽

A. Chattopadyay ◽

H. Sroussi ◽

...

Keyword(s):

Herpes Simplex ◽

Epstein Barr Virus ◽

Human Herpesvirus ◽

Human Herpesvirus 8 ◽

Viral Pathogens ◽

Herpesvirus 8 ◽

Varicella Zoster ◽

Barr Virus ◽

Epstein Barr ◽

Simplex Virus

HIV infection is commonly associated with activation and dissemination of several other viral pathogens, including herpes simplex virus 1/2, human cytomegalovirus, human herpesvirus 8, Epstein-Barr virus, Varicella Zoster virus, and human papillomavirus, which behave as opportunistic agents and cause various diseases in immunocompromised hosts. The increased frequency and severity of diseases caused by these viruses in HIV-infected individuals is due mainly to dysfunction of both the adaptive and innate immune responses to viral pathogens. In addition, molecular interactions between HIV and these opportunistic viruses are likely to play critical roles in the progression of disease, including neoplasia. This report reviews the critical aspects of HIV interaction with opportunistic viruses, including Epstein-Barr virus, human cytomegalovirus, herpes simplex virus, Varicella Zoster virus, human herpesvirus 8, and human papillomavirus.

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Regulation of Viral Infection in Diabetes

Biology ◽

10.3390/biology10060529 ◽

2021 ◽

Vol 10 (6) ◽

pp. 529

Author(s):

Seiho Nagafuchi

Keyword(s):

Influenza Virus ◽

Epstein Barr Virus ◽

Hepatitis Virus ◽

Human Herpesvirus ◽

Mumps Virus ◽

Human Herpesvirus 6 ◽

Varicella Zoster ◽

Barr Virus ◽

Epstein Barr ◽

Virus Influenza

Though there is no ‘Diabetes Virus’, multiple agents such as mumps virus, rubella virus, influenza virus, type A hepatitis virus, enterovirus, rotavirus, cytomegalovirus, varicella-zoster virus, human herpesvirus 6, Epstein-Barr virus, and also SARS-CoV-2 have been reported to be associated to diabetes [...]

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