Amino acid polymorphism in the reverse transcriptase region of hepatitis B virus and the relationship with nucleos(t)ide analogues treatment for preventing mother-to-infant transmission

2014 ◽  
Vol 86 (8) ◽  
pp. 1288-1295 ◽  
Author(s):  
Jie Chen ◽  
Ling Yan ◽  
Feng-Cai Zhu ◽  
Jian-Xun Liu ◽  
Rong-Cheng Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Amino Acid Polymorphism ◽  
Reverse Transcriptase Region ◽  
B Virus ◽  
The Relationship

scholarly journals A Single Amino Acid in the Reverse Transcriptase Domain of Hepatitis B Virus Affects Virus Replication Efficiency

2001 ◽  
Vol 75 (23) ◽  
pp. 11827-11833 ◽  
Author(s):  
Xu Lin ◽  
Zheng-Hong Yuan ◽  
Li Wu ◽  
Jian-Ping Ding ◽  
Yu-Mei Wen
Keyword(s):  
Amino Acid ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Single Amino Acid ◽  
Structural Basis ◽  
Replication Efficiency ◽  
P Gene ◽  
B Virus ◽  
The Difference

ABSTRACT To explore functional domains in the hepatitis B virus (HBV) polymerase, two naturally occurring HBV isolates (56 and 2-18) with 98.7% nucleic acid sequence homology but different replication efficiencies were studied. After transfection into HepG2 cells, HBV DNA isolated from intracellular virus core particles was much higher in 56-transfected cells than in cells transfected with 2-18. The structural basis for the difference in replication efficiency between these two isolates was studied by functional domain gene substitution. The complete polymerase (P) gene and its gene segments coding for the terminal protein (TP), spacer (SP), reverse transcriptase (RT), and RNase H in 2-18 were separately replaced with their counterparts from 56 to construct full-length chimeric genomes. Cell transfection analysis revealed that substitution of the complete P gene of 2-18 with the P gene from 56 slightly enhanced viral replication. The only chimeric genome that regained the high replication efficiency of the original 56 isolate was the one with substitution of the RT gene of 2-18 with that from 56. Within the RT region, amino acid differences between isolates 2-18 and 56 were located at positions 617 (methionine versus leucine), 652 (serine versus proline), and 682 (valine versus leucine). Point mutation identified amino acid 652 as being responsible for the difference in replication efficiency. Homologous modeling studies of the HBV RT domain suggest that the mutation of residue 652 from proline to serine might affect the conformation of HBV RT which interacts with the template-primer, leading to impaired polymerase activity.

scholarly journals Naturally occurring mutations in the reverse transcriptase region of hepatitis B virus polymerase from treatment-naïve Korean patients infected with genotype C2

2017 ◽  
Vol 23 (23) ◽  
pp. 4222 ◽  
Author(s):  
Ji-Eun Kim ◽  
So-Young Lee ◽  
Hong Kim ◽  
Ki-Jeong Kim ◽  
Won-Hyeok Choe ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Korean Patients ◽  
Naturally Occurring ◽  
Reverse Transcriptase Region ◽  
B Virus ◽  
Treatment Naïve

scholarly journals Mutation analysis of hepatitis B virus reverse transcriptase region among untreated chronically infected patients in Ahvaz city (South-West of Iran)

2013 ◽  
Vol 31 (4) ◽  
pp. 360 ◽  
Author(s):  
M Makvandi ◽  
A Samarbaf-Zadeh ◽  
E Hajiani ◽  
A Shayesteh ◽  
A Masjedizadeh ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Mutation Analysis ◽  
South West ◽  
Reverse Transcriptase Region ◽  
B Virus

The amino acid substitutions rtP177G and rtF249A in the reverse transcriptase domain of hepatitis B virus polymerase reduce the susceptibility to tenofovir

2013 ◽  
Vol 97 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Bo Qin ◽  
Bettina Budeus ◽  
Liang Cao ◽  
Chunchen Wu ◽  
Yun Wang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Amino Acid Substitutions ◽  
Reverse Transcriptase Domain ◽  
B Virus

Association between lamivudine sensitivity and the number of substitutions in the reverse transcriptase region of the hepatitis B virus polymerase

2007 ◽  
Vol 14 (9) ◽  
pp. 661-666 ◽  
Author(s):  
K. Fukai ◽  
K.Y. Zhang ◽  
F. Imazeki ◽  
T. Kurihara ◽  
R. Mikata ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Reverse Transcriptase Region ◽  
B Virus

Early dynamic changes of quasispecies in the reverse transcriptase region of hepatitis B virus in telbivudine treatment

2021 ◽  
Vol 195 ◽  
pp. 105178
Author(s):  
Xia Tang ◽  
Wenxun Huang ◽  
Juan Kang ◽  
Keyue Ding
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Dynamic Changes ◽  
Reverse Transcriptase Region ◽  
B Virus

scholarly journals Amino Acid Polymorphism in Hepatitis B Virus Associated with Functional Cure

2021 ◽  
Author(s):  
Takashi Honda ◽  
Norie Yamada ◽  
Asako Murayama ◽  
Masaaki Shiina ◽  
Hussein Hassan Aly ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Functional Cure ◽  
Amino Acid Polymorphism ◽  
B Virus
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