Optimization of indigo production by a newly isolatedPseudomonassp. QM

2012 ◽  
Vol 52 (6) ◽  
pp. 687-694 ◽  
Author(s):  
Yuanyuan Qu ◽  
Qiao Ma ◽  
Xuwang Zhang ◽  
Hao Zhou ◽  
Xinliang Li ◽  
...  
Keyword(s):  
2000 ◽  
Vol 31 (1) ◽  
pp. 5-9 ◽  
Author(s):  
B. Bhushan ◽  
S.K. Samanta ◽  
R.K. Jain

2019 ◽  
Vol 20 (24) ◽  
pp. 6148 ◽  
Author(s):  
Nikola Lončar ◽  
Hugo L. van Beek ◽  
Marco W. Fraaije

Indigo is currently produced by a century-old petrochemical-based process, therefore it is highly attractive to develop a more environmentally benign and efficient biotechnological process to produce this timeless dye. Flavin-containing monooxygenases (FMOs) are able to oxidize a wide variety of substrates. In this paper we show that the bacterial mFMO can be adapted to improve its ability to convert indole into indigo. The improvement was achieved by a combination of computational and structure-inspired enzyme redesign. We showed that the thermostability and the kcat for indole could be improved 1.5-fold by screening a relatively small number of enzyme mutants. This project not only resulted in an improved biocatalyst but also provided an improved understanding of the structural elements that determine the activity of mFMO and provides hints for further improvement of the monooxygenase as biocatalyst.


2017 ◽  
Author(s):  
Prima Astuti Handayani ◽  
Megawati ◽  
Kusdianto ◽  
Deny Aditia Nugraha ◽  
Lilis Novitasari

2010 ◽  
Vol 50 (3) ◽  
pp. 290-293 ◽  
Author(s):  
Juan Pablo Riva Mercadal ◽  
Paula Isaac ◽  
Faustino Siñeriz ◽  
Marcela Alejandra Ferrero

2016 ◽  
Vol 73 (2) ◽  
pp. 248-254 ◽  
Author(s):  
Lei Cheng ◽  
Sheng Yin ◽  
Min Chen ◽  
Baoguo Sun ◽  
Shuai Hao ◽  
...  

2003 ◽  
Vol 19 (6) ◽  
pp. 1792-1797 ◽  
Author(s):  
L.G. Angelini ◽  
E. Campeol ◽  
S. Tozzi ◽  
K.G. Gilbert ◽  
D.T. Cooke ◽  
...  

2014 ◽  
Vol 19 (2) ◽  
pp. 154-163
Author(s):  
Norbert Herber

Awa-ai is the indigo plant and dye made famous hundreds of years ago in Japan by the people living and working in the region now known as Tokushima Prefecture. This article explores the core concepts that link the aged traditions of indigo production, processing and dyeing with contemporary sound practice, and outlines the facets of a collaboration in which disparate fields not only coexisted, but used their technological and cultural differences to strengthen one another. Live field recordings, audio interviews and the sounds of Awa indigo production and practice were used in a large-scale, transcontinental installation which featured over 200 pieces of indigo-dyed cloth and multichannel, interactive sound. The collaborative nature of this project allowed the artists involved to understand and conceptualise their work in new ways, and can serve as an example for the ways in which syncretic exploration energises creative thinking and output.


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