Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan

2019 ◽  
Vol 59 (10) ◽  
pp. 1004-1015 ◽  
Author(s):  
Bassem M. Salama ◽  
Wafaa A. Helmy ◽  
Tamer I. M. Ragab ◽  
Mamdouh M. Ali ◽  
Hanan A. A. Taie ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Low Molecular Weight

scholarly journals Characterization of dacC, Which Encodes a New Low-Molecular-Weight Penicillin-Binding Protein in Bacillus subtilis

1998 ◽  
Vol 180 (18) ◽  
pp. 4967-4973 ◽  
Author(s):  
Lotte B. Pedersen ◽  
Thomas Murray ◽  
David L. Popham ◽  
Peter Setlow
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Binding Protein ◽  
Low Molecular Weight ◽  
Penicillin Binding Protein ◽  
Wild Type ◽  
Genome Sequencing Project ◽  
Penicillin Binding Proteins ◽  
Sequencing Project

ABSTRACT The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed thatdacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor ςH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacCinsertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression ofdacC in Escherichia coli showed that this gene encodes an ∼59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed.

Isolation and Characterization of Low Molecular Weight Ribonucleic Acid Species from Bacillus subtilis*

Biochemistry ◽  
1967 ◽  
Vol 6 (1) ◽  
pp. 258-265 ◽  
Author(s):  
Pierre Morell ◽  
Issar Smith ◽  
David Dubnau ◽  
Julius Marmur
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Ribonucleic Acid ◽  
Low Molecular Weight ◽  
Isolation And Characterization
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