scholarly journals Extracellular matrix expression of human tenocytes in three-dimensional air-liquid and PLGA cultures compared with tendon tissue: Implications for tendon tissue engineering

2010 ◽  
Vol 28 (9) ◽  
pp. 1170-1177 ◽  
Author(s):  
Christiane Stoll ◽  
Thilo John ◽  
Michaela Endres ◽  
Christian Rosen ◽  
Christian Kaps ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Matrix Expression

Three-Dimensional-Construct Bioreactor Conditioning in Human Tendon Tissue Engineering

2011 ◽  
Vol 17 (19-20) ◽  
pp. 2561-2572 ◽  
Author(s):  
Colin Y.L. Woon ◽  
Armin Kraus ◽  
Shyam S. Raghavan ◽  
Brian C. Pridgen ◽  
Kai Megerle ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering ◽  
Human Tendon

DIFFERENCES BETWEEN PROTEIN EXPRESSION AND EXTRACELLULAR MATRIX STATE ON UNIAXIAL STRETCHING FOR TENOGENIC DIFFERENTIATION

2014 ◽  
Vol 14 (02) ◽  
pp. 1450025 ◽  
Author(s):  
YASUYUKI MORITA ◽  
SATOSHI SUZUKI ◽  
YANG JU ◽  
NAOKI KAWASE
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Protein Expression ◽  
Type I Collagen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Tendon Tissue ◽  
Uniaxial Stretching ◽  
Expression Levels ◽  
Tendon Tissue Engineering

There have been no previous reports of tendon tissue engineering using mesenchymal stem cells (MSCs) with regard to quantitative evaluation of protein expression levels and observation of derived extracellular matrix (ECM) state. Therefore, we approached tendon tissue engineering from both perspectives. Human bone marrow MSCs (hBMSCs) were subjected to 8% or 10% cyclic stretching at 1 Hz to promote differentiation into tenocytes and ECM production. The type I collagen (Col I) and Tenascin-C (Tnc) protein expression levels were evaluated quantitatively by enzyme-linked immunosorbent assay (ELISA). Confocal fluorescence microscopy was employed to observe the derived ECM state. Col I state derived from 10%-stretched cells as ECM was elongated like actual tendon ECM, although the quantitative protein expression levels were slightly higher in 8%-stretched cells. The results suggested that the optimal uniaxial stretching ratio was different between protein expression levels and derived ECM state. Therefore, it is important to pay attention to both protein expression levels and ECM state in tendon tissue engineering.

In vitrotwo-dimensional and three-dimensional tenocyte culture for tendon tissue engineering

2013 ◽  
Vol 10 (3) ◽  
pp. E216-E226 ◽  
Author(s):  
Yiwei Qiu ◽  
Xiao Wang ◽  
Yaonan Zhang ◽  
Andrew J. Carr ◽  
Liwei Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

Direct E-jet printing of three-dimensional fibrous scaffold for tendon tissue engineering

2015 ◽  
Vol 105 (3) ◽  
pp. 616-627 ◽  
Author(s):  
Yang Wu ◽  
Zuyong Wang ◽  
Jerry Ying Hsi Fuh ◽  
Yoke San Wong ◽  
Wilson Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Tendon Tissue ◽  
Fibrous Scaffold ◽  
Tendon Tissue Engineering ◽  
Jet Printing

Tendon Tissue Engineering: Mechanism and Effects of Human Tenocyte Coculture With Adipose-Derived Stem Cells

2018 ◽  
Vol 43 (2) ◽  
pp. 183.e1-183.e9 ◽  
Author(s):  
Chao Long ◽  
Zhen Wang ◽  
Anais Legrand ◽  
Arhana Chattopadhyay ◽  
James Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering
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